Rabbit anti human p npm thr199 polyclonal antibody

Manufactured by Cell Signaling Technology

Rabbit anti-human p-NPM (Thr199) polyclonal antibody is a laboratory reagent used to detect and quantify the phosphorylation of Nucleophosmin (NPM) at the threonine 199 residue in human samples. It is a polyclonal antibody raised in rabbits.

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Lab products found in correlation

Rabbit anti human npm polyclonal antibody, by Cell Signaling Technology (2 mentions) Rabbit anti human cleaved caspase 3 asp175 polyclonal antibody, by Cell Signaling Technology (1 mentions) Rabbit anti human ki 67 clone sp6 monoclonal antibody, by Thermo Fisher Scientific (1 mentions) Brightvision horseradish peroxidase conjugated goat anti rabbit polyclonal secondary antibody, by Immunologic (1 mentions) Rabbit anti human parp polyclonal antibody, by Cell Signaling Technology (1 mentions) Mouse anti human α tubulin clone dm1a monoclonal antibody, by Cell Signaling Technology (1 mentions)

2 protocols using rabbit anti human p npm thr199 polyclonal antibody

Immunohistochemical Analysis of NPM, p-NPM, Ki-67, and Cleaved Caspase-3

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The following antibodies were used: rabbit anti-human NPM polyclonal antibody (1:1000, Cell Signaling Technology); rabbit anti-human p-NPM (Thr199) polyclonal antibody (1:400, Cell Signaling Technology); rabbit anti-human Ki-67 (clone SP6) monoclonal antibody (1:1000, Thermo Scientific), rabbit anti-human cleaved caspase 3 (Asp175) polyclonal antibody (1:100, Cell Signaling Technology) and BrightVision horseradish peroxidase-conjugated goat anti-rabbit polyclonal secondary antibody (undiluted; 30 min; Immunologic). See Supplementary Materials and Methods for a detailed protocol.

Dolman M.E., Poon E., Ebus M.E., den Hartog I.J., van Noesel C.J., Jamin Y., Hallsworth A., Robinson S.P., Petrie K., Sparidans R.W., Kok R.J., Versteeg R., Caron H.N., Chesler L, & Molenaar J.J. (2015). Cyclin-dependent kinase inhibitor AT7519 as a potential drug for MYCN-dependent neuroblastoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(22), 5100-5109.

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Western blot analysis of cell signaling

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The following antibodies were used: mouse anti-human Rb (clone G3-245) monoclonal antibody (1:1000, BD Biosciences); rabbit anti-human p-Rb (Thr⁸²¹) polyclonal antibody (1:1000, BioSource International); mouse anti-human MYCN (clone B8.4.B) monoclonal antibody (1:5000, BD Biosciences); rabbit anti-human PARP polyclonal antibody (1:2000, Cell Signaling Technology); rabbit anti-human NPM polyclonal antibody (1:1000, Cell Signaling Technology); rabbit anti-human p-NPM (Thr199) polyclonal antibody (1:500, Cell Signaling Technology); rabbit anti-human β-actin (clone 13E5) monoclonal antibody (1:1000, Cell Signaling Technology); mouse anti-human β-actin (clone AC-15) monoclonal antibody (1:20000, Abcam); mouse anti-human α-tubulin (clone DM1A) monoclonal antibody (1:1000, Cell Signaling Technology) and IRDye 800CW goat anti-rabbit and goat anti-mouse secondary antibodies (1:5000, Li-COR). See Supplementary Materials and Methods for a detailed protocol.

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