Eagle s minimum essential medium

Manufactured by Corning

Sourced in United States

Eagle's minimum essential medium is a cell culture medium formulated to support the growth and maintenance of a variety of cell lines. It provides the essential nutrients and components necessary for cell proliferation and viability in vitro.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions) Complete growth medium, by Celprogen (3 mentions) Sh sy5y cell, by American Type Culture Collection (3 mentions) Ham s f 12k nutrient mixture, by Corning (3 mentions) Human endothelial progenitor cells, by Celprogen (3 mentions) Fetal bovine serum (fbs), by Corning (3 mentions) Penicillin streptomycin, by Corning (3 mentions) Mcf 7, by American Type Culture Collection (3 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Hep3b cell line, by American Type Culture Collection (2 mentions) Iscove s modified dulbecco s medium, by Thermo Fisher Scientific (2 mentions) Mycoplasma detection kit, by Lonza (2 mentions) F 12k medium, by American Type Culture Collection (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Mccoy s 5a medium, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Omega Scientific (2 mentions) Non essential amino acids (neaa), by Corning (2 mentions) 1640 medium, by Corning (2 mentions) Gm12878, by Coriell Institute for Medical Research (2 mentions) Hct116, by Corning (2 mentions)

31 protocols using eagle s minimum essential medium

Hepatic Stellate Cell Co-culture with Aptamers

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A 0.4 micron transwell system was used for co-culture experiments, with hepatic stellate LX2 cells seeded on the bottom well and HepG2 cells on the top transwell at a 1:1 ratio (30% confluency) in 6-well culture dishes. Cells were grown in standard Eagles Minimum Essential Medium (Corning Cellgro, Mediatech, Inc, Manassas, VA) with 10% fetal bovine serum at 37°C and 5% CO₂. Experimental groups received 100 nM of OPN-R3 aptamer (APT) or mutant aptamer (MuAPT) daily. Cells were cocultured for a total of 72 hours, after which HepG2 cells were removed and RNA from LX2 cells was acquired using Trizol.
The pharmacologic properties and sequences of OPN-R3 aptamer (APT) and mutant aptamer (MuAPT) have been previously published [32 (link)].

Arffa M.L., Zapf M.A., Kothari A.N., Chang V., Gupta G.N., Ding X., Al-Gayyar M.M., Syn W., Elsherbiny N.M., Kuo P.C, & Mi Z. (2016). Epigallocatechin-3-Gallate Upregulates miR-221 to Inhibit Osteopontin-Dependent Hepatic Fibrosis. PLoS ONE, 11(12), e0167435.

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Cell Culture and Maintenance Protocol

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Vero cells (ATCC #CCL-81) were maintained in Eagle’s minimum essential medium (Corning Cellgro; Manassas) with 5% fetal bovine serum (Sigma Aldrich; St. Louis, Missouri) and 1% penicillin–streptomycin solution (HyClone; Logan, Utah). HUH-7 and A549 cells (ATCC #CCL-185) cells were maintained in DMEM with 5% FBS (Sigma Aldrich; St. Louis, Missouri) and 1% penicillin–streptomycin solution (HyClone; Logan, Utah). Cells were incubated at 37°C, 5% CO₂ and split twice weekly to maintain subconfluency.

Franco E.J., Rodriquez J.L., Pomeroy J.J., Hanrahan K.C, & Brown A.N. (2018). The effectiveness of antiviral agents with broad-spectrum activity against chikungunya virus varies between host cell lines. Antiviral Chemistry & Chemotherapy, 26, 2040206618807580.

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Culturing and Xenografting Human Glioblastoma

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Human glioblastoma cells (U-87 MG) were obtained from ATCC^® (VA, US) and cultured in Eagle's Minimum Essential Medium (Corning Cellgro, VA, US) containing 10% FBS, 1% Penicillin/Streptavidin solution, 2 mM L-glutamine, 1 mM sodium pyruvate and 0.075% (w/v) sodium bicarbonate. They were incubated in a humidified 5% CO₂ atmosphere and used between passages 8 and 15.
For in vivo experiments, 6-8 week old female Hsd:Athymic Nude-Foxn1^nu mice were purchased from Envigo (IN, US). All animal experiments were performed in accordance with institutional guidelines and approved by the IACUC of MSK, and followed NIH guidelines for animal welfare.

Haedicke K., Brand C., Omar M., Ntziachristos V., Reiner T, & Grimm J. (2017). Sonophore labeled RGD: a targeted contrast agent for optoacoustic imaging. Photoacoustics, 6, 1-8.

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EGCG Dose-Dependent Effects on HepG2 Cells

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HepG2 cell line (ATCC# HB-8065) was obtained from American Type Tissue Collection (ATCC, Manassas, VA), seeded at approximately 1.0 × 10⁶ cells/plate, and grown in standard Eagles Minimum Essential Medium (Corning Cellgro, Mediatech, Inc, Manassas, VA) with 10% fetal bovine serum at 37°C and 5% CO₂. In the following experiments, cells were treated with EGCG (Sigma Aldrich Inc, Saint Louis, MO) at concentrations of 0.02 μg/mL, 0.2 μg/mL, 2 μg/mL, and 20 μg/mL every 12h for 24h unless otherwise specified.

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Culturing Human Neuroblastoma and Endothelial Progenitor Cells

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Human neuroblastoma cell line SH-SY5Y cells (ATCC, MD, USA) were cultured in complete medium containing Ham’s F-12 K Nutrient Mixture and Eagle’s Minimum Essential Medium (Corning Cellgro, VA, USA) with 10% fetal bovine serum (HyClone, PA, USA), 100 IU/ml penicillin, and 100 mg/ml streptomycin in an atmosphere of 5% CO₂ at 37 °C. The medium was changed every other day, the cells were passaged when the density reached around 75%. The 12–16 passages of the cells were used in the present study.
Human endothelial progenitor cells (Celprogen, Torrance, CA) were cultured in complete growth medium (Celprogen, Torrance, CA) under standard cell culture conditions (5% CO₂, 37 °C) according to the manufacturer’s protocol. The medium was changed every other day, and the cells were passaged when the density reached around 80%. The cells underwent 8–11 passages in the present study.

Li Y., Wang J., Chen S., Wu P., Xu S., Wang C., Shi H, & Bihl J. (2020). miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway. Stem Cell Research & Therapy, 11, 330.

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Epigallocatechin Gallate Inhibits Liver Cancer

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HepG2, Hep3B, and MHCC 97-H cell lines were seeded at approximately 1.0 × 10⁶ cells/plate and grown in standard Eagles Minimum Essential Medium (Corning Cellgro, Mediatech, Inc, Manassas, VA) with 10% fetal bovine serum, glutamine, and penicillin streptomycin at 37°C and 5% CO₂. In the following experiments, cells were treated with EGCG at concentrations 20 ng/mL, 200 ng/mL, 2 μg/mL, and 20 μg/mL for 48 hours unless otherwise specified. The only experiment performed on Hep3B cells was enzyme-linked immunosorbent assay (ELISA) for OPN.

Zapf M.A., Kothari A.N., Weber C.E., Arffa M.L., Wai P.Y., Driver J., Gupta G.N., Kuo P.C, & Mi Z. (2015). Green tea component epigallocatechin-3-gallate decreases expression of osteopontin via a decrease in mRNA half-life in cell lines of metastatic hepatocellular carcinoma. Surgery, 158(4), 1039-1048.

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Culturing Human Cell Lines for Research

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Human neuroblastoma cell line SH-SY5Y cells (ATCC, MD, USA) were cultured in complete medium containing Ham's F-12K Nutrient Mixture and Eagle's Minimum Essential Medium (Corning Cellgro, VA, USA) with 10% fetal bovine serum (HyClone, PA, USA), 100 IU/ml penicillin, and 100 mg/ml streptomycin in an atmosphere of 5% CO 2 at 37°C. The medium was changed every other day, the cells were passaged when the density reached around 75%. The 12 -16 passages of the cells were used in the present study.
Human endothelial progenitor cells (Celprogen, Torrance, CA) were cultured in complete growth medium (Celprogen; Torrance, CA) under standard cell culture conditions (5% CO 2 , 37°C) according to the manufacturer's protocol. The medium was changed every other day, and the cells were passaged when the density reached around 80%. The cells underwent 8-11 passages in the present study.

Li Y., Wang J., Chen S., Wu P., Xu S., Wang C., Shi H., & Chen J. (2020). MiR-137 Boosts the Neuroprotective Effect of Endothelial Progenitor Cell Derived-Exosomes in Oxyhemoglobin Treated SH-SY5Y Cells Partially via COX2/PGE2 Pathway.

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In Vitro Cultivation of Neuroblastoma and Endothelial Progenitor Cells

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Cell culture Human neuroblastoma cell line SH-SY5Y cells (ATCC, MD, USA) were cultured in complete medium containing Ham's F-12K Nutrient Mixture and Eagle's Minimum Essential Medium (Corning Cellgro, VA, USA) with 10% fetal bovine serum (HyClone, PA, USA), 100 IU/ml penicillin, and 100 mg/ml streptomycin in an atmosphere of 5% CO 2 at 37°C. The medium was changed every other day, the cells were passaged when the density reached around 75%. The 12 -16 passages of the cells were used in the present study.
Human endothelial progenitor cells (Celprogen, Torrance, CA) were cultured in complete growth medium (Celprogen; Torrance, CA) under standard cell culture conditions (5% CO 2 , 37°C) according to the manufacturer's protocol. The medium was changed every other day, and the cells were passaged when the density reached around 80%. The cells underwent 8-11 passages in the present study.

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Culturing Primary, Metastatic, and Normal Colon Cells

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Primary (SW480, ATCC^® CCL-228 ™) and metastatic (SW620, ATCC^® CCL-227 ™) colon cancer cell lines and normal colon cell line (CCD-18co, ATCC^® CRL-1459 ™) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). SW480 and SW620 cells were maintained in Leibovitz L-15 medium (Corning Life Sciences, Corning, NY, USA) supplemented with 10% fetal bovine serum and 1% antibiotics; penicillin and streptomycin, in a 37 °C incubator without CO₂. CCD-18co cells were maintained in Eagles Minimum Essential Medium (EMEM) (Corning Life Sciences, USA) supplemented with 10% fetal bovine serum and 1% antibiotics, in a 37 °C incubator with CO₂. Cells were frequently monitored to ensure a normal and consistent morphology. While maintaining proper aseptic techniques, the cells were subcultured every 2–3 days or upon 90% confluency and the culture medium replenished within a biosafety cabinet (Labculture © Class II, Type A2 Biological Safety, Esco Technologies Inc., Horsham, PA, USA).

Zainal Abidin S.A., Rajadurai P., Hoque Chowdhury M.E., Othman I, & Naidu R. (2018). Cytotoxic, Anti-Proliferative and Apoptosis Activity of l-Amino Acid Oxidase from Malaysian Cryptelytrops purpureomaculatus (CP-LAAO) Venom on Human Colon Cancer Cells. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(6), 1388.

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Synthesis and Purification of Modified mRNA

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DOPE, DMG-PEG₂₀₀₀, and C18-CONH-PEG₂₀₀₀ and other lipid materials were acquired from Avanti Polar Lipids Inc. (Alabaster, AL). Eagle’s minimum essential medium (EMEM) and other cell culture supplies were purchased from Corning Incorporated (Corning, NY). Quant-iT RiboGreen RNA reagent and Gibco heat-inactivated fetal bovine serum (FBS) were acquired from Thermo Fisher Scientific (Waltham, MA). All the other chemical reagents were obtained from Sigma-Aldrich or Alfa Aesar and used without further purifications.
mRNAs were synthesized through IVT using the AmpliScribe T7-Flash Transcription Kit (Lucigen Corporation, Middleton, WI) and followed by the addition of a Cap1 structure using the Vaccinia Capping System and mRNA Cap 2′-O-methyltransferase (New England Biolabs, Ipswich, MA). Pseudouridine-5′-triphosphate (TriLink BioTechnologies, San Diego, CA) was used for synthesizing ψ modified mRNA. mRNAs were purified through RNA Clean & Concentrator Kits (Zymo Research, Irvine, CA) before diluted in Tris-EDTA (TE) buffer for applications.

Zhang X., Zhao W., Nguyen G.N., Zhang C., Zeng C., Yan J., Du S., Hou X., Li W., Jiang J., Deng B., McComb D.W., Dorkin R., Shah A., Barrera L., Gregoire F., Singh M., Chen D., Sabatino D.E, & Dong Y. (2020). Functionalized lipid-like nanoparticles for in vivo mRNA delivery and base editing. Science Advances, 6(34), eabc2315.

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