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Adipogenic differentiation medium

Manufactured by STEMCELL
Sourced in Canada

Adipogenic differentiation medium is a cell culture medium designed to promote the differentiation of cells into adipocytes (fat cells). It contains specific growth factors and supplements that stimulate the differentiation process.

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4 protocols using adipogenic differentiation medium

1

Osteogenic and Adipogenic Differentiation

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The cells were cultured up to 80%–90% confluence and changed to the osteogenic differentiation medium or the adipogenic differentiation medium (STEMCELL Technologies, Vancouver, BC, Canada). Osteoblastic differentiation was analyzed by alizarin red staining. Adipocytic differentiation was identified by Oil Red-O staining.
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2

Adipocyte Differentiation from iPSCs

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D-iPSCs and H-iPSCs were differentiated into adipocytes. Mesoderm differentiation was induced on day 0 in the mesoderm induction medium (STEMCELL Technologies). On day 4, adipocyte differentiation was induced in an adipogenic differentiation medium (STEMCELL Technologies) with 1% L-glutamine. After 2 to 3 weeks, adipocytes had formed, and the presence of lipid droplets (LDs) was identified by Oil Red O (ORO) staining.
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3

Isolation and Characterization of hUMSCs

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hUMSCs were extracted using the tissue block culture attachment method, as previously reported [19 (link),20 (link)]. The preparation of P5 hUMSCs was conducted in a GMP facility following good clinical practice (GCP) guidelines. The identification of hUMSCs was detected by flow cytometry (BD Biosciences, FACSAria2, USA). The antibodies used in this experiment included CD73-BV421 (cat.562430, BD Biosciences, USA), CD105-APC (cat. 562408, BD Biosciences, USA), CD90-FITC (cat.555595, BD Biosciences, USA), HLA-DR-PE (cat. 555812, BD Biosciences, USA), CD34-PerCP-Cy™5.5 (cat. 347203; BD Biosciences, USA), and CD45-FITC (cat.555482; BD Biosciences, USA). Osteogenic differentiation medium (catalog#05465, STEMCELL Technologies, China), adipogenic differentiation medium (catalog#05412, STEMCELL Technologies, China), and chondrogenic differentiation medium (catalog#05455, STEMCELL Technologies, China) were used to assess the osteogenic, adipogenic, and chondrogenic differentiation potential of hUMSCs, following the manufacturer’s instructions. Oil Red O solution (O1391, Sigma-Aldrich, USA), Alizarin Red S (A5533, Sigma-Aldrich, USA), and Alcian Blue staining solution (TMS-010-C, Sigma-Aldrich, USA) were used for adipogenic, osteogenic, and chondrogenic staining, respectively, according to the manufacturer’s instructions.
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4

Adipogenic and Osteogenic Differentiation

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Adipogenic differentiation assay was performed by culturing in the adipogenic differentiation medium (STEMCELL Technologies, Canada) for 21 days and then identified by Oil Red-O staining. Osteoblastic differentiation of CBMSCs and ASCs was induced by the osteogenic differentiation medium (STEMCELL Technologies, Canada) for 14 days and then analyzed by Alizarin Red staining.
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