Hypersense dnp

Manufactured by Oxford Instruments

Sourced in United Kingdom

HyperSense DNP is a dynamic nuclear polarization (DNP) system designed for enhancing the sensitivity of nuclear magnetic resonance (NMR) spectroscopy. The core function of the HyperSense DNP is to transfer polarization from unpaired electrons to nuclear spins, resulting in a significant increase in the signal-to-noise ratio of NMR experiments.

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Lab products found in correlation

Ox063 radical, by GE Healthcare (2 mentions) Dotarem, by Guerbet (2 mentions) Prohance, by Bracco (1 mentions) Trityl radical ox063, by Oxford Instruments (1 mentions) 1 13c pyruvate, by Cambridge Isotopes (1 mentions) Avance 3 hd, by Bruker (1 mentions) Inveon pet ct system, by Siemens (1 mentions)

8 protocols using hypersense dnp

Hyperpolarized 13C MRI of Tumor Metabolism

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Hyperpolarized ¹³C MRI experiments were performed on a 3T MRI
scanner(MR solutions Inc.) using a 17 mm-diameter home-built
¹H/¹³C coil. A 96 mM hyperpolarized [1-¹³C]
pyruvate solution from a Hypersense DNP polarizer(Oxford Instruments) was
administrated via a tail veil cannula(12 μL/g body weight).
¹³C two dimensional spectroscopic images were acquired with a
32×32 mm² field of view in a 8 mm axial slice through the
tumor.

Yamamoto K., Brender J.R., Seki T., Kishimoto S., Oshima N., Choudhuri R., Adler S.S., Jagoda E., Saito K., Devasahayam N., Choyke P.L., Mitchell J.B, & Krishna M.C. (2020). Molecular Imaging of the Tumor Microenvironment reveals the Relationship between Tumor Oxygenation, Glucose Uptake and Glycolysis in Pancreatic Ductal Adenocarcinoma. Cancer research, 80(11), 2087-2093.

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Hyperpolarized 13C Pyruvate MRI for In Vivo Metabolic Imaging

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1-¹³C pyruvic acid (30 μL), containing 15 mM OXO63 and 2.5 mM gadolinium chelate ProHance (Bracco Diagnostics, Milano, Italy), was hyperpolarized at 3.35T and 1.4K using the Hypersense DNP polarizer (Oxford Instruments, Abingdon, UK) according to the manufacturer's instructions. After 60-90 min, the hyperpolarized sample was rapidly dissolved in 4.5 mL of a superheated alkaline buffer that consisted of 50 mM Tris(hydroxymethyl)aminomethane, 75 mM NaOH, and 100 mg/L ethylenediaminetetraacetic acid. The hyperpolarized 1-¹³C pyruvate solution (96 mM) was intravenously injected through a catheter placed in the tail vein of the mouse (12 μL/g body weight). Hyperpolarized ¹³C MRI studies were performed on a 3T scanner (MR Solutions, Guildford, UK) using a home-built ¹³C solenoid leg coil. After the rapid injection of hyperpolarized 1-¹³C pyruvate, spectra were acquired every second for 240 seconds using a single pulse sequence. Data were analyzed in a model free approach using the lactate/pyruvate ratio, calculated from the areas under the curves of the 1-¹³C lactate peak and the 1-¹³C pyruvate peak [15 (link)].

Neveu M.A., Preter G.D., Joudiou N., Bol A., Brender J.R., Saito K., Kishimoto S., Grégoire V., Jordan B.F., Krishna M.C., Feron O, & Gallez B. (2016). Multi-modality imaging to assess metabolic response to dichloroacetate treatment in tumor models. Oncotarget, 7(49), 81741-81749.

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Hyperpolarized Pyruvate Metabolism Study

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Pyruvate samples were prepared by mixing 14 M [1-¹³C] pyruvate (Cambridge Isotopes), 15 mM OX063 radical (GE Healthcare) and 1.5 mM of Dotarem (Guerbet LLC). 22 μL aliquots were polarized using a commercial HyperSense DNP polarizer (Oxford Instruments) for approximately one hour. Samples were melted with 4 mL dissolution buffer (80 mM NaOH, 40 mM Trizma buffer, 50 mM NaCl and 0.1 mg/L EDTA) at 10 bar pressure and 180 ºC to yield 80 mM isotonic neutral polarized [1-¹³C] pyruvate at 37 ºC. Polarization was estimated to be 20±6% for the studies based on the solid-state build up curve and separate measurements. Samples were transferred to the animal within approximately 10 seconds of dissolution and 6.7±1.9 ml/kg were administered through the tail vein over 10±2 seconds. This was followed by a 300-μL saline dose over 2 seconds to flush the pyruvate from the catheter’s dead volume. To minimize respiratory motion during data acquisition, a 10-second breath-hold was applied using the ventilator and data acquisition was initiated 12±1 seconds after the end of injection.

Pourfathi M., Xin Y., Kadlecek S.J., Cereda M.F., Profka H., Hamedani H., Siddiqui S.M., Ruppert K., Drachman N.A., Rajaei J.N, & Rizi R.R. (2017). In-vivo imaging of the progression of acute lung injury using hyperpolarized [1-13C] pyruvate. Magnetic resonance in medicine, 78(6), 2106-2115.

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Hyperpolarized [2-13C]Pyruvate Production

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A mixture of 14-M [2-¹³C]-labeled pyruvic acid (Sigma-Aldrich Co., St. Louis, MO, USA) and 15-mM trityl radical OX063 (Oxford Instruments, UK) was polarized using a HyperSense DNP polarizer (Oxford Instruments, UK) by irradiating the sample with microwaves (94.076 GHz) at ∼1.4 K. When the solid-state polarization reached more than 98% of the estimated maximum polarization level, the sample was dissolved with a buffer solution (40 mM tris[hydoroxymethyl]aminomethane, 125 mM NaOH, 100 mg/L ethylenediaminetetraacetic acid (EDTA) and 50 mM NaCl), resulting in a 125-mM solution of [2-¹³C]pyruvate with pH ∼7.5.

Park J.M., Josan S., Jang T., Merchant M., Watkins R., Hurd R.E., Recht L.D., Mayer D, & Spielman D.M. (2015). Volumetric Spiral Chemical Shift Imaging of Hyperpolarized [2-13C]Pyruvate in a Rat C6 Glioma Model. Magnetic resonance in medicine, 75(3), 973-984.

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Hyperpolarized 19F NMR Acquisition

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Hyperpolarization was generated on ¹⁹F spins in a HyperSense DNP polarizer (Oxford Instruments, Abingdon, UK) by irradiating with microwaves of 94.055 GHz and 100 mW power, at a temperature of 1.4 K. After 20 min of the polarization, the samples were dissolved in a stream of 4 mL of buffer that had been pre-heated until a pressure of 5 bar was reached at an approximate temperature of 385 K. Dissolved samples were automatically loaded into the injection loop.

Kim Y., Liu M, & Hilty C. (2016). Parallelized Ligand Screening Using Dissolution Dynamic Nuclear Polarization. Analytical chemistry, 88(22), 11178-11183.

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Dynamic Nuclear Polarization of 1-13C-Pyruvate

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Dynamic Nuclear Polarization of 1-¹³C-pyruvate was performed using a HyperSense DNP polarizer (Oxford Instruments, Abingdon, UK) at an excitation frequency of 94.080 GHz and temperature of ~1.4 K. DNP was performed on a stock mixture of 14 M [1-¹³C]-pyruvate (Cambridge Isotopes, Tewksbury, MA), 15 mM OX063 radical (GE Healthcare) and 1.5 mM of Dotarem (Guerbet LLC, Princeton, NJ). 22 μL of stock solution were melted in 4 mL or 8 mL of dissolution buffer (80 mM NaOH, 40 mM Trizma (pH = 7.6), 50 mM NaCl and 0.42 mM EDTA), for in vivo or in vitro experiments for in vivo or in vitro experiments respectively, at 10-bar pressure and 180 °C to yield 80 mM isotonic, neutral hyperpolarized 1-¹³C-pyruvate. For in vitro experiments, 6.3 mL of hyperpolarized 1-¹³C-pyruvate was pumped into the cell mass at 7 mL/min. For in vivo studies, 6.7 mL/kg of the sample was administered through a tail vein catheter over 13 seconds by hand injection, followed by administration of 300-μL saline over 2 seconds to flush the pyruvate from the catheter’s dead volume.

Perkons N.R., Kiefer R.M., Noji M.C., Pourfathi M., Ackerman D., Siddiqui S., Tischfield D., Profka E., Johnson O., Pickup S., Mancuso A., Pantel A., Denburg M.R., Nadolski G.J., Hunt S.J., Furth E.E., Kadlecek S, & Gade T.P. (2020). Hyperpolarized Metabolic Imaging Detects Latent Hepatocellular Carcinoma Domains Surviving Locoregional Therapy. Hepatology (Baltimore, Md.), 72(1), 140-154.

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Metabolic Imaging of Immune Checkpoint Blockade

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C57BL/6, ITK-SYK^CD4-CreERT2 or ITK-SYK^CD4-CreERT2;Pdcd1^−/− mice received a single dose of 2 mg tamoxifen. For PET–CT imaging, mice were injected with [¹⁸F]FDG (~12 MBq), and tracer uptake was measured after 90 min using a preclinical Siemens Inveon PET–CT system. Splenic standardized uptake values were calculated using Inveon Research Workplace software. Hyperpolarized ¹³C MRI of the animals was performed using a preclinical 7-T magnetic resonance scanner (Agilent–GE Discovery MR901 magnet and gradient system, Bruker AVANCE III HD electronics). The animals were injected with hyperpolarized [1-¹³C]pyruvate (250 µl, 80 mM, HyperSense DNP, Oxford Instruments), and both [1-¹³C]pyruvate and [1-¹³C]lactate were recorded in vivo using a static 2D FID–CSI (matrix size, 18 × 12; FOV, 30 mm × 20 mm; slice thickness, 3 mm; flip angle, 12°; number of points, 128; bandwidth, 2,000 Hz). T₂-weighted images (¹H-RARE; matrix size, 150 × 100; FOV, 30 mm × 20 mm; slice thickness, 1 mm) were recorded for co-registration and determination of spleen size. Peak heights for [1-¹³C]pyruvate versus [1-¹³C]lactate were determined and summed over the spleen region of interest using MATLAB code developed in house.

Wartewig T., Daniels J., Schulz M., Hameister E., Joshi A., Park J., Morrish E., Venkatasubramani A.V., Cernilogar F.M., van Heijster F.H., Hundshammer C., Schneider H., Konstantinidis F., Gabler J.V., Klement C., Kurniawan H., Law C., Lee Y., Choi S., Guitart J., Forne I., Giustinani J., Müschen M., Jain S., Weinstock D.M., Rad R., Ortonne N., Schilling F., Schotta G., Imhof A., Brenner D., Choi J, & Ruland J. (2023). PD-1 instructs a tumor-suppressive metabolic program that restricts glycolysis and restrains AP-1 activity in T cell lymphoma. Nature Cancer, 4(10), 1508-1525.

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Hyperpolarized 13C NMR via DNP

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Hyperpolarization was achieved using a HyperSense DNP polarizer (Oxford Instruments, UK) with microwave irradiation at 93.938 GHz and 100 mW at 1.4 K. Subsequent ¹³C NMR was acquired with a 1.4 T Spinsolve 60 Carbon High Performance benchtop NMR apparatus (Magritek, New Zealand).

Kondo Y., Saito Y., Elhelaly A.E., Hyodo F., Nishihara T., Itoda M., Nonaka H., Matsuo M, & Sando S. (2021). Evaluation of enzymatic and magnetic properties of γ-glutamyl-[1-13C]glycine and its deuteration toward longer retention of the hyperpolarized state. RSC Advances, 11(59), 37011-37018.

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