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Anti rabbit antibodies 926 32211

Manufactured by LI COR

Anti-rabbit antibodies (926-32211) are secondary antibodies designed for use in Western blotting, ELISA, and other immunoassay applications. These antibodies are specific to rabbit primary antibodies and are conjugated with an infrared dye for detection on LI-COR imaging systems.

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2 protocols using anti rabbit antibodies 926 32211

1

Kinase Inhibition Signaling Assay

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All materials were obtained from ThermoFisher Scientific unless otherwise noted. Apyrase and 2-MeSADP, were purchased from Sigma (St. Louis, MO). PAR 4 peptide AYPGKF-NH2 and PAR 1 peptide SLFLLRN-NH2 were synthesized by GenScript (Piscataway, NJ). Anti-Akt (Ser473) cat#4060 was obtained from Cell Signaling (Danvers, MA). Antibody to total Akt cat#TA504230 was purchased from Origene (Rockville, MD). Blocking buffer, infrared dye-labeled goat anti-mouse (926-68020) and anti-rabbit antibodies (926-32211) were purchased from LI-COR (Lincoln, NE). PI103 was purchased from Tocris Bioscience (Minneapoli, MN)
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2

Quantifying Active Rap1 and Akt

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All materials were obtained from ThermoFisher Scientific unless otherwise noted. Apyrase and 2-MeSADP, were purchased from Sigma (St. Louis, MO). Active Rap1 detection kit cat#8818 and anti-Akt(Ser473) cat# 4060 were obtained from Cell Signaling (Danvers, MA). Antibody to total Akt cat#TA504230 was purchased from Origene (city state). Blocking buffer, infrared dye-labeled goat anti-mouse (926-68020) and anti-rabbit antibodies (926-32211) were purchased from LI-COR (Lincoln, NE).
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