Determination of lung cytokine levels was performed on homogenized whole lungs from infected animals harvested four- and 12- hours post-infection and homogenized in PBS. Samples were normalized to total protein prior to analysis. Determination of systemic cytokine levels was performed on serum isolated from infected mice at the indicated time points. Bone marrow-derived macrophage cytokines were quantified from cell culture supernatant at four hours post-infection. All cytokines were quantified using the MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore) on a Luminex Flexmap 3D platform (Luminex) according to the manufacturer’s recommended protocol.
Map mouse cytokine chemokine magnetic bead panel
Manufactured by Merck Group
Sourced in United States
The MAP Mouse Cytokine/Chemokine Magnetic Bead Panel is a multiplex assay designed for the simultaneous quantitative measurement of multiple mouse cytokines and chemokines in a single sample. The panel utilizes color-coded magnetic beads coated with specific antibodies to capture and detect the target analytes.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using map mouse cytokine chemokine magnetic bead panel
1
Quantifying Cytokine Levels in Lung Infections
2
Evaluating Stress Biomarkers in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All mice were returned to their original cages after completing the MWM test (without any further stress) and were sacrificed 15 days later for blood and tissue sampling. Brain tissue was quickly removed and stored in a refrigerator for RNA-scope and western blotting.
Whole blood was collected via the eyeball. Serum was prepared by centrifuging blood samples for 5 min at 4000 rpm (4°C). Approximately 100 μl of serum was collected from each mouse and analyzed for corticosterone (CORT) levels with a quantitative ELISA (Demeditec Corticosterone rat/mouse ELISA, Demeditec Diagnostics, Germany). The MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore, USA) was used to determine the levels of TNF-α and IL-1β.
Whole blood was collected via the eyeball. Serum was prepared by centrifuging blood samples for 5 min at 4000 rpm (4°C). Approximately 100 μl of serum was collected from each mouse and analyzed for corticosterone (CORT) levels with a quantitative ELISA (Demeditec Corticosterone rat/mouse ELISA, Demeditec Diagnostics, Germany). The MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore, USA) was used to determine the levels of TNF-α and IL-1β.
3
Multiplex Cytokine Analysis of Murine Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein levels of IL-1β, IL-6, MIP-2, TNF-α, and IL-10 in whole protein lysates or plasma were measured using a customized Milliplex Mouse Cytokine Immunoassay Kit (2620525)/MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel, MCYTOMAG-70K with Analyzer 3.1 Luminex 200 machine (Millipore, USA). Data were analyzed on corresponding software according to the manufacturer’s instructions.
4
Cytokine Profiling from Murine Splenocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For ex vivo stimulation, splenocytes were cultured at a concentration of 1 × 106cells/ml either in the absence or presence of LPS (1 μg/ml) for 18 h, at 37°C, 5% CO2. The supernatant was collected to determine cytokine profile. The MAP Mouse Cytokine/Chemokine Magnetic Bead panel (Millipore, USA) was used to assess the levels of IL-1β, IL-6, IL-10, TNF-α, and IFN-γ. The samples were prepared in duplicate, as per manufacturer's instructions, and run on the Bioplex 200 system (Biorad, USA) equipped with Bio-Plex Manager™ software. Cytokine concentrations were automatically calculated using a six-point standard curve fitted with a five-parameter logistic regression algorithm. The kit's lowest detection thresholds were as follows: IL-1β, 5.4 pg/ml; IL-6, 1.1 pg/ml; TNF-α, 2.3 pg/ml, and IL-10, 2.0 pg/ml.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!