pEGFP-C1 plasmid vector for eukaryotic expression was purchased from Clontech (Palo Alto, CA, USA). pGFP-Klf9 (pCMV-AC-GFP-Klf9) plasmid was purchased from OriGene (RG210147, OriGene Technologies, Inc., Rockville, MD, USA). pcDNA3-EGFP-C4-Nrf2 (pEGFP-Nrf2) plasmid were purchased from Addgene (Watertown, MA, USA). Control shRNA (sc-108060) and Nrf2 ShRNA (sc-37030-SH) plasmids were purchased from Santa Cruz Biotechnology. For experimentation, LECs were transfected with Control ShRNA or Nrf2 ShRNA using the Neon Transfection System (Invitrogen, Waltham, MA, USA).
Control shrna sc 108060
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Control shRNA (sc-108060) is a non-targeting short hairpin RNA (shRNA) sequence designed as a negative control for RNA interference experiments. The sequence does not target any known gene in human, mouse or rat cells.
Automatically generated - may contain errors
Lab products found in correlation
Neon transfection system, by Thermo Fisher Scientific (2 mentions)
Nrf2 shrna sc 37030 sh, by Santa Cruz Biotechnology (2 mentions)
Pegfp c1 plasmid vector, by Takara Bio (1 mentions)
Pcdna3.1 vector, by GenePharma (1 mentions)
Ags cell line, by American Type Culture Collection (1 mentions)
Shrna plasmid transfection reagent sc 108061, by Santa Cruz Biotechnology (1 mentions)
Ges 1 cell line, by Shanghai Cell Bank (1 mentions)
Control sirna sc 37007, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
5 protocols using control shrna sc 108060
1
Eukaryotic Expression Plasmids and Transfection
2
Modulation of SPRR2B Expression in Gastric Cancer Cells
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Human gastric epithelium GES-1 cell line was purchased from the Shanghai Cell Bank of the Chinese Academy of Science (Shanghai, China) and kept in DMEM medium. AGS cell line was obtained from ATCC and kept in F-12K medium. MKN28 and MKN45 cells were obtained from ATCC and maintained in RPMI 1640 medium. All medium was supplemented with 10% fetal bovine serum and penicillin-streptomycin antibiotics.
Human SPRR2B-shRNA (sc-88,442-SH) and control-shRNA (sc-108,060) plasmids were obtained from Santa Cruz Biotechnology. MKN45 cells were transfected with shRNA plasmids using shRNA Plasmid Transfection Reagent (sc-108,061, Santa Cruz).19 (link) Transfected cells were selected by using puromycin. Specific siRNAs targeting MDM2 (sc-29,394) and SPRR2B (sc-88,442) were used for transient transfection, using non-specific scramble siRNA (sc-37,007) as negative control. For overexpression assays, pcDNA3.1-vector, pcDNA3.1-SPRR2B, and pcDNA3.1-SPRR2B-optimized plasmids were synthesized by Gene Pharma (Shanghai, China). The pcDNA3.1-SPRR2B-optimized (pcDNA-SPRR2B°pt) construct was generated by mutating the SPRR2B-siRNA targeted region of wild type SPRR2B and was used for rescue experiments.
Human SPRR2B-shRNA (sc-88,442-SH) and control-shRNA (sc-108,060) plasmids were obtained from Santa Cruz Biotechnology. MKN45 cells were transfected with shRNA plasmids using shRNA Plasmid Transfection Reagent (sc-108,061, Santa Cruz).19 (link) Transfected cells were selected by using puromycin. Specific siRNAs targeting MDM2 (sc-29,394) and SPRR2B (sc-88,442) were used for transient transfection, using non-specific scramble siRNA (sc-37,007) as negative control. For overexpression assays, pcDNA3.1-vector, pcDNA3.1-SPRR2B, and pcDNA3.1-SPRR2B-optimized plasmids were synthesized by Gene Pharma (Shanghai, China). The pcDNA3.1-SPRR2B-optimized (pcDNA-SPRR2B°pt) construct was generated by mutating the SPRR2B-siRNA targeted region of wild type SPRR2B and was used for rescue experiments.
3
Stable Nrf2 knockdown in SRA-hLECs
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Control shRNA (sc-108060) and Nrf2 shRNA (sc-37030-SH) plasmids were purchased from Santa Cruz Biotechnology, Dallas, TX, USA. SRA-hLECs were transfected with Control shRNA and Nrf2 shRNA. To make stable cell lines transfectants were selected with the selection marker puromycin. SRA-hLECs were transfected by using the Neon Transfection System (Invitrogen, Waltham, MA, USA) as described in our published protocol [21 (link),74 (link),81 (link)].
4
ZKSCAN3 Gene Silencing in Prostate Cancer Cell Lines
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Human prostatic carcinoma cell lines (LNCaP/VCaP/PC3/DU145/C4-2) originally obtained from the American Tissue Type Collection (Manassas, VA, USA) and recently authenticated by the institutional core facility were maintained in RPMI 1640 (Mediatech, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS). Gene silencing was achieved by transfection of a ZKSCAN3-siRNA (sc-95093), a control-siRNA (sc-37007), a ZKSCAN3-shRNA (sc-95093-SH), or a control-shRNA (sc-108060) (all from Santa Cruz Biotechnology, Dallas, TX, USA), as we described previously [43 (link),47 (link),48 (link)].
5
Stable Gastric Cancer Cell Lines
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ERp19 shRNA (sc-60597-SH) or control shRNA (sc-108060) plasmids (Santa Cruz Biotechnology) were transfected into gastric cancer cells using Lipofectamine 2000 (Invitrogen). ERp19 cDNA ORF was cloned into the pHBLV-IRES-ZsGreen-PGK-Puro plasmid (HanbioTM) for lentivirus production. Stable cell lines were screened by purimycine and identified by western blotting.
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