S nitrosoglutathione

Manufactured by Cayman Chemical

S-nitrosoglutathione is a laboratory reagent used in research applications. It is a nitrosothiol compound that can be used to study the biological functions and properties of nitric oxide.

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Lab products found in correlation

Sodium ascorbate, by Merck Group (1 mentions) Neocuproine, by Merck Group (1 mentions) Sequencing grade trypsin, by Promega (1 mentions) N 6 biotinamido hexyl 3 2 pyridyldithio propionamide hpdp biotin, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Merck Group (1 mentions) Lactate dehydrogenase, by Merck Group (1 mentions) Nω nitro l arginine methyl ester hydrochloride l name, by Merck Group (1 mentions) Irdye 680rd goat anti rabbit igg, by LI COR (1 mentions) Criterion tgxtm precast gel, by Bio-Rad (1 mentions) Dulbecco s modification of eagle s medium dmem, by Corning (1 mentions) Irdye 800cw goat anti mouse igg, by LI COR (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Bortezomib, by LC Laboratories (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)

2 protocols using s nitrosoglutathione

S-Nitrosoglutathione Proteomic Analysis

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Sodium ascorbate, neocuproine, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), ethylenediaminetetraacetic acid (EDTA), diethylene triamine pentaacetic acid (DTPA), copper chloride, bovine serum albumin (BSA), methanol, N-ethylmeleimide (NEM), trifluoroacetic acid, acetonitrile, and all other chemicals, unless specified, were from Sigma (St. Louis, MO). N-(6-[biotinamido] hexyl)-3′-(2′-pyridyldithio) propionamide (biotin-HPDP) was from Thermo Scientific (Rockford, IL). S-nitrosoglutathione (GSNO) was from Cayman (Ann Arbor, MI). Sequencing-grade trypsin was purchased from Promega Corp. (Madison, WI). SILAC RPMI-1640 (deficient in lysine and arginine) was from Invitrogen (Carlsbad, CA). L-¹²C₆¹⁴N₄-Arg, L-¹²C₆¹⁴N₂-Lys, L-¹³C₆¹⁵N₄-Arg, and L-¹³C₆¹⁵N₂-Lys were purchased from Cambridge Isotope Laboratories (Andover, MA).

Zhang H.H., Lechuga T.J., Chen Y., Yang Y., Huang L, & Chen D.B. (2016). Quantitative Proteomics Analysis of VEGF-Responsive Endothelial Protein S-Nitrosylation Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and LC-MS/MS. Biology of Reproduction, 94(5), 114.

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Cellular Signaling Pathway Protocol

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Fetal Bovine Serum was purchased from Atlanta Biologicals (Flowery Branch, GA). Dulbecco's Modification of Eagle's Medium (DMEM) was from Corning (Corning, NY). Bortezomib and calpeptin were obtained from LC laboratories (Woburn, MA) and Calbiochem (San Diego, CA), respectively. Dipropylenetriamine NONOate (DPTA) and S-nitrosoglutathione (GSNO) were from Cayman Chemicals (Ann Arbor, MI). CriterionTM TGXTM Precast Gels (8–16%) were obtained from Bio-Rad Laboratories (Hercules, CA). Lactate dehydrogenase, N^ω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), cycloheximide, danazole, and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO). Mouse monoclonal antibodies to the V5-peptide (catalog # V8012), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, catalog # MAB374) and actin (catalog # GTX109639) were purchased from Sigma-Aldrich (St. Louis, MO), Millipore (Billerica, MA), and GeneTex (Irvine, CA), respectively. The monoclonal CYP2J2 antibody was obtained from Abnova (Taipei, Taiwan, catalog # H00001573-M01). IRDye® 680RD Goat anti-Rabbit IgG and IRDye® 800CW Goat anti-Mouse IgG were from LI-COR Biosciences (Lincoln, NE).

Park J.W., Lee C.M., Cheng J.S, & Morgan E.T. (2018). Posttranslational regulation of CYP2J2 by nitric oxide. Free radical biology & medicine, 121, 149-156.

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