Mouse igg1k isotype control

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Mouse IgG1k isotype control is a laboratory reagent used as a negative control in immunoassays and flow cytometry experiments. It is an immunoglobulin G1 (IgG1) antibody from mouse with a kappa light chain. The isotype control serves as a reference to distinguish specific antibody binding from non-specific background signals.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Isotype controls (54 citations) Mouse IgG1k (6 citations) Isotype control mouse IgG1k-PE (2 citations)

6 protocols using mouse igg1k isotype control

Flow Cytometric Analysis of DR4 and DR5

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were collected with cell dissociation buffer and spun at 1000 rpm for 3 minutes. Cells were resuspended in staining buffer (2% FBS, 0.02% sodium azide, and PBS) and incubated with anti-DR4-PE or anti-DR5-PE (eBioscience, San Diego, CA, USA) for 1 hour in the dark at 4°C; a mouse IgG1 K isotype control (eBioscience) was used to compensate for any nonspecific binding. Cells were washed twice with staining buffer and resuspended in staining buffer for analysis. DR4 and DR5 membrane expressions were analyzed on a BD FACSCanto II flow cytometer using FACSDiva software. Histograms were prepared employing Flowing Software 2. Each experiment was performed in triplicate, and 3 independent experiments were conducted for each cell line to obtain the fold increase in DR4 or DR5 cell surface expression relative to the vehicle-treated control ± SEM.

Manouchehri J.M., Turner K.A, & Kalafatis M. (2018). TRAIL-Induced Apoptosis in TRAIL-Resistant Breast Carcinoma Through Quercetin Cotreatment. Breast Cancer : Basic and Clinical Research, 12, 1178223417749855.

+ Open protocol

+ Expand

Multicolor Flow Cytometry for Human Cell Engraftment in Humanized Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

To detect human cells engrafted in HUMAMICE, multi-colors cytometric analysis was performed using BD LSR Fortessa, according to the manufacturer’s protocol but with a minor modification. HUMAMICE were sacrificed and the spleens were removed at three different times after hPBMCs were transplanted. Splenocytes were collected and lysed by ACK lysis buffer, counted and incubated with an appropriate volume of antibodies for 1 hour at 4°C and then subjected to flow cytometry analysis.
Commonly used antibodies included: Anti-Human CD45 FITC, Clone: 2D1, (ebioscience9011‐9459); isotype control, Mouse IgG1 K Isotype Control (ebioscience, FITC 11–4714) ; Anti-Mouse CD45 eFluor^® 450 (ebioscience, 48-0451-82) ; isotype control, Mouse IgG2b K Isotype Control eFluor^® 450 (ebioscience, 48-4732-82) ; Anti-Human CD3 APC-eFluor^® 780, Clone: UCHT1, (ebioscience, 47–0038) ; Mouse IgG1 K Isotype Control APC- eFluor^® 780 (ebioscience, 47–4714) ; Anti-Human CD4 PerCP-Cyanine5.5, Clone: OKT4 (OKT-4), (ebioscience, 45–0048); isotype control, Mouse IgG2b K Isotype Control PerCP-Cyanine5.5 (cat. 45–4732) ; Anti-Human CD8a PE-Cyanine7, Clone: SK1, (ebioscience, 25–0087); isotype control, Mouse IgG1 K Isotype Control PE-Cyanine7 (ebioscience, 25–4714) ; Anti-Human CD19 APC, Clone: 2H7, (ebioscience, 17–0209); isotype control, Mouse IgG1 K Isotype Control APC (ebioscience, 17–4714).

Zeng Y., Liu B., Rubio M.T., Wang X., Ojcius D.M., Tang R., Durrbach A., Ru Z., Zhou Y, & Lone Y.C. (2017). Creation of an immunodeficient HLA-transgenic mouse (HUMAMICE) and functional validation of human immunity after transfer of HLA-matched human cells. PLoS ONE, 12(4), e0173754.

+ Open protocol

+ Expand

Sulf-2 Interactome Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Amicon Ultra-15 (Millipore), Dynabeads^™ M-280 sheep anti-Mouse IgG (Invitrogen), Anti-Sulf-2 monoclonal antibodies (QED Bioscience), Anti-LG3BP monoclonal antibody (Proteintech), Mouse IgG1 k Isotype control (eBioscience), BSA (NEB), Recombinant Sulf-2 protein (produced in house), Recombinant human galectin 3 binding protein (R&D Systems, and produced in-house), QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies), 25 kDa polyethylenimine (Polysciences), Custom heavy isotope labeled Sulf-2 peptide (Biosynth), Trypsin/Lys-C protease (Thermo Scientific), Empore C18 cartridge (CDS Analytical), Nano-flow C18 columns, and other reagents and chemicals were obtained from Thermo Scientific.

Panigrahi A., Benicky J., Aljuhani R., Mukherjee P., Nováková Z., Bařinka C, & Goldman R. (2023). Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatse Sulf-2. bioRxiv.

+ Open protocol

+ Expand

Eosinophil Siglec Engagement Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Freshly isolated eosinophils (1.5 × 10⁵/150 μL in culture medium) were blocked in 5% goat serum in PBS prior to incubation for 30 minutes at 4°C in 96-well U shape plates (Nunc, Roskilde, Denmark) in the presence of anti-Siglec-7 (QA79, eBiosciences), anti-Siglec-8 (7C9, Biolegend), or matched control antibodies (mouse IgG1k isotype control, eBiosciences) (0.62–5 μg/mL). After washing, crosslinker (F(ab′)₂fragment goat anti-mouse IgG (H+L) (10 μg/mL), Jackson Laboratories, West Grove, PA, US) and rhGM-CSF (50 ng/mL) were added simultaneously and the cells were incubated for 40 minutes (eosinophil peroxidase, EPX release) or overnight (CD69 expression and cytokine release) at 37°C, 5% CO₂ in phenol-free RPMI 5% FCS. Supernatants were collected and stored at −20°C. Anti-CD300a (1 μg/mL, from hybridoma #12, provided by Prof. O. Mandelboim) was used as a positive control for inhibition in all experiments.

Legrand F., Landolina N., Zaffran I., Emeh R.O., Chen E., Klion A.D, & Levi-Schaffer F. (2019). Siglec-7 on peripheral blood eosinophils: surface expression and function. Allergy, 74(7), 1257-1265.

+ Open protocol

+ Expand

Immunophenotypic Characterization of Bone Marrow Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow derived MSCs were studied for the
expression of CD45 as the hematopoietic marker,
along with CD73 and CD90 as the MSC surface
markers. CD73, a PE conjugated antibody, (BD
Biosciences, CA, USA) and FITC-conjugated goat
anti-mouse IgG antibodies for CD45 and CD90
(FITC conjugated) were used. Mouse IgG1 K
isotype control (eBiosciences, CA, USA), mouse
IgG2a K isotype control FITC (eBiosciences, CA,
USA), and donkey anti-mouse IgG (H+L) PE
(eBiosciences, CA, USA) were used as secondary
antibodies for detection of the selected markers.
Unstained cells were used for gating in the flow
cytometric analysis. We counted 15000 events
for each antibody. Data were analyzed by FlowJo
software version 7.6.4 (17 (link)).

Jazayeri M., Shokrgozar M.A., Haghighipour N., Bolouri B., Mirahmadi F, & Farokhi M. (2016). Effects of Electromagnetic Stimulation on Gene Expression of Mesenchymal Stem Cells and Repair of Bone Lesions. Cell Journal (Yakhteh), 19(1), 34-44.

+ Open protocol

+ Expand

Multiparametric Immunofluorescence Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

After fixation with 2% formaldehyde for 10 min, permeabilization with 70% cold ethanol, and a blocking step, cells were incubated simultaneously for 4 h with the following antibodies diluted 1/100: mouse IgG1k isotype control (eBioscience, 14-4714-82), XP rabbit mAb isotype control IgG (Cell Signaling, 3900S), anti-CD47 B6H12 IgG1k mouse (eBioscience, 14-0479), thrombospondin-1 (Cell Signaling, 37879), and anti-Ki67 IgG rabbit (Cell Signaling, 9129S). After washing three times with PBS–Tween 0.02%, cells were incubated with secondary antibodies diluted 1/200: goat anti-mouse IgG secondary antibody Alexa 488 (Invitrogen, A11001) and goat anti-rabbit IgG secondary antibody Alexa 568 (Invitrogen, A11011). Cells were washed three times with PBS–Tween 0.02% and covered with Prolong Diamond with DAPI.

Guillon J., Petit C., Moreau M., Toutain B., Henry C., Roché H., Bonichon-Lamichhane N., Salmon J.P., Lemonnier J., Campone M., Verrièle V., Lelièvre E., Guette C, & Coqueret O. (2019). Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment. Cell Death & Disease, 10(3), 199.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!