Methacrylic acid maac

Methoxy poly(ethylene glycol) (mPEG) (M_W 5000), poly(ethylene glycol) (PEG) (M_W 400), dipalmitoylphosphatidylcholine (DPPC), cholesterol, 4,4′-azobis(4-cyanovaleric acid) (ABCPA), l-lysine (Lys), and phosphotungstic acid (PTA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methacrylic acid (MAAc) and ammonium sulfate ((NH₄)₂SO₄) were obtained from Acros Organics (Geel, Belgium). N-hydroxysuccinimide (NHS ester) was purchased from Alfa Aesar (Heysham, Lancashire, UK). Doxorubicin (Dox) was purchased from LC Laboratories (Woburn, MA, USA). Methanol and dichloromethane (DCM) were purchased from ECHO (Miaoli, Taiwan). The fluorescent dye cyanine 5.5-NHS ester (Cy5.5-NHS ester) was obtained from Lumiprobe (Hunt Valley, MD, USA). Fetal bovine serum (FBS) and LysoTracker Red DND-99 fluorescent dye were received from Thermo Fisher Scientific (Waltham, MA, USA). The PD-10 desalting column and Sephadex G 50 column were purchased from GE Healthcare Life Sciences (Little Chalfont, Buckinghamshire, UK).

(3-Carboxypropyl) triphenyl phosphonium bromide (CTPP) (No. 349720), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine semisynthetic (DPPC) (No. P0763), 2-amino-ethanethiol hydrochloride, 4,4′-azobis(4-cyanovaleric acid) (ABCPA) (No. 11590), cholesterol (No. C3045), N,N′-dicyclohexylcarbodiimide (DCC) (No. D80002), hydrated p-toluenesulfonic acid (PTSA) (No. 402885), 2-aminoethanethiol hydrochloride (AET-HCl) (No.122920), N-hydroxysuccinimide (NHS) (No.130672), poly(ethylene glycol) methyl ether (mPEG; MW = 5000) (No. 81323), L-histidine (No. H6034), and thiazolyl blue tetrazolium bromide (MTT) (No. M5655) were purchased from Sigma-Aldrich. 1-Ethyl-3-(3-dimethylamino-propyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) (No. D1601) was purchased from TLC. 4-Dimethylaminopyride (DMAP) (No. A13016) was purchased from Alfa Aesar. Methacrylic acid (MAAc) (No. AC168310250) was purchased from ACROS. All organic solvents were purchased from Merck.