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Spe cleanup

Manufactured by Thermo Fisher Scientific
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SPE cleanup is a laboratory equipment used for sample preparation. It facilitates the purification and extraction of analytes from complex matrices. The core function of SPE cleanup is to selectively retain and concentrate the target compounds while removing unwanted interferences.

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Lab products found in correlation

2 protocols using spe cleanup

1

Quantitative Liver Proteomics Workflow

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Targeted proteomics was applied in homogenized liver tissue by the isotopically labeled peptide standards (G6PC; GLGVDLLWTLEK, CYP8B1; VFGYQSVDGDHR, ChREBP; LGFDTLHGLVSTLSAQPSLK, CYP7A1; LSSASLNIR, oxysterol 7α-hydroxylase [CYP7B1]; YITFVLNP FQYQYVTK, sterol 27-hydroxylase [CYP27A1]; LYPVVPTNSR, cytochrome P450, family 2, subfamily c, polypeptide 70 [CYP2C70]; TDSSLLSR), containing 13C-labeled lysine/arginine (PolyQuant GmbH, Bad Abbach, Germany), according to the workflow described.(21 (link)) The following alterations were made: Lipids were extracted from liver homogenates with diethyl ether before the proteomics workflow and the concentrations were related to the total peptide content, which was determined by a colorimetric peptide assay after tryptic digestion and SPE cleanup (Thermo Scientific). Concentrations of endogenous peptides were calculated from the known concentration of the standard and expressed in fmol/μg of total peptide and expressed relative to the values in the control group.
Hoogerland J.A., Lei Y., Wolters J.C., de Boer J.F., Bos T., Bleeker A., Mulder N.L., van Dijk T.H., Kuivenhoven J.A., Rajas F., Mithieux G., Haeusler R.A., Verkade H.J., Bloks V.W., Kuipers F, & Oosterveer M.H. (2019). Glucose‐6‐Phosphate Regulates Hepatic Bile Acid Synthesis in Mice. Hepatology (Baltimore, Md.), 70(6), 2171-2184.
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2

Quantitative Liver Proteome Profiling

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Targeted proteomics was applied in homogenized liver tissue by the isotopically labeled peptide standards (G6PC; GLGVDLLWTLEK, CYP8B1; VFGYQSVDGDHR, ChREBP; LGFDTLHGLVSTLSAQPSLK, CYP7A1; LSSASLNIR, oxysterol 7α‐hydroxylase [CYP7B1]; YITFVLNPFQYQYVTK, sterol 27‐hydroxylase [CYP27A1]; LYPVVPTNSR, cytochrome P450, family 2, subfamily c, polypeptide 70 [CYP2C70]; TDSSLLSR), containing 13C‐labeled lysine/arginine (PolyQuant GmbH, Bad Abbach, Germany), according to the workflow described.21 The following alterations were made: Lipids were extracted from liver homogenates with diethyl ether before the proteomics workflow and the concentrations were related to the total peptide content, which was determined by a colorimetric peptide assay after tryptic digestion and SPE cleanup (Thermo Scientific). Concentrations of endogenous peptides were calculated from the known concentration of the standard and expressed in fmol/µg of total peptide and expressed relative to the values in the control group.
Hoogerland J.A., Lei Y., Wolters J.C., de Boer J.F., Bos T., Bleeker A., Mulder N.L., van Dijk T.H., Kuivenhoven J.A., Rajas F., Mithieux G., Haeusler R.A., Verkade H.J., Bloks V.W., Kuipers F, & Oosterveer M.H. (2019). Glucose‐6‐Phosphate Regulates Hepatic Bile Acid Synthesis in Mice. Hepatology (Baltimore, Md.), 70(6), 2171-2184.
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