Ti s er motorized stage with encoders

Manufactured by Nikon

Sourced in Japan

The Ti-S-ER motorized stage with encoders is a precision laboratory equipment component designed for microscope applications. It provides accurate and reliable positioning and control of the sample stage.

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Lab products found in correlation

Eclipse ti inverted fluorescence microscope, by Nikon (2 mentions) Intensilight, by Nikon (2 mentions) Cfi plan fluor 10 0.30 n a objective lens, by Nikon (2 mentions) Orca r2, by Hamamatsu Photonics (2 mentions) Gfp hyq, by Nikon (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Nikon (1 mentions)

2 protocols using ti s er motorized stage with encoders

Inverted Fluorescence Microscopy Protocol

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The sample containing cells was placed on the stage of a Nikon ECLIPSE Ti inverted fluorescence microscope equipped with a CFI plan fluor 10 × /0.30 N.A. objective lens (Nikon, Tokyo, Japan), CCD camera (ORCA-R2; Hamamatsu Photonics, Hamamatsu City, Japan), mercury lamp (Intensilight; Nikon), XYZ automated stage (Ti-S-ER motorized stage with encoders; Nikon), and filter cubes for fluorescence channels (DAPI, GFP HYQ, TRITC; Nikon). For image acquisition, the exposure times were set to 200 ms for DAPI, 200 ms for GFP HYQ (for ALB), 50 ms for GFP HYQ (for CK19), and 200 ms for TRITC (for CYP3A7).

Yoshimoto K., Minier N., Yang J., Imamura S., Stocking K., Patel J., Terada S., Hirai Y, & Kamei K.I. (2020). Recapitulation of Human Embryonic Heartbeat to Promote Differentiation of Hepatic Endoderm to Hepatoblasts. Frontiers in Bioengineering and Biotechnology, 8, 568092.

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Microscopic imaging of fluorescent cells

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The sample containing cells was placed on the stage of a Nikon ECLIPSE Ti inverted fluorescence microscope equipped with a CFI plan fluor 10×/0.30 N.A. objective lens (Nikon, Tokyo, Japan), CCD camera (ORCA-R2; Hamamatsu Photonics, Hamamatsu City, Japan), mercury lamp (Intensilight; Nikon), XYZ automated stage (Ti-S-ER motorized stage with encoders; Nikon), and filter cubes for fluorescence channels (DAPI and GFP HYQ; Nikon). For image acquisition, the exposure times were set at 500 ms for DAPI and 500 ms for GFP HYQ (for A1AT).

Kamei K.I., Yoshioka M., Terada S., Tokunaga Y, & Chen Y. (2019). Three-dimensional cultured liver-on-a-Chip with mature hepatocyte-like cells derived from human pluripotent stem cells. Biomedical microdevices, 21(3).

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