Igor pro 6.34a

Manufactured by Wavemetrics

Sourced in United States, Japan

Igor Pro 6.34A is a data analysis and graphing software developed by Wavemetrics. It provides tools for data acquisition, analysis, and visualization across various scientific disciplines. The software offers a comprehensive suite of functions to manage, process, and present data in a flexible and customizable manner.

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Lab products found in correlation

Spss statistics 17, by IBM (1 mentions) Originpro 9, by OriginLab (1 mentions) Agilent 8800 triple quadrupole icp ms, by Agilent Technologies (1 mentions) Microm hm550, by Thermo Fisher Scientific (1 mentions) Sigmaplot 12, by Merck Group (1 mentions) Clampex 10, by Molecular Devices (1 mentions) Clampfit 10, by Molecular Devices (1 mentions) Axopatch 200b, by Molecular Devices (1 mentions) Axopatch 200b amplifier, by Molecular Devices (1 mentions) Ac240, by Olympus (1 mentions) Mfp 3d atomic force microscope, by Oxford Instruments (1 mentions)

Close spelling variants of this product

Igor Pro (1089 citations) Igor Pro 6 (343 citations) Igor 6 (19 citations)

7 protocols using igor pro 6.34a

Quantifying Synaptic Responses and Analysis

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Evoked synaptic responses were averaged per cell over >10 alternating repetitions in Igor Pro 6.34A (Wavemetrics, USA). Response area was determined by subtracting a baseline (10 ms prior to event onset) from the response for a maximum of 100 ms or until the response dropped below baseline. For peak amplitude analysis, the maximum deviation from baseline was used. In the case of feedback inhibition, the response to the second pulse stimulus was baseline-corrected and subsequently subtracted from the first pulse, as this subsequent response does not contain nicotine-dependent polysynaptic input (Supplementary Fig. 1d). Statistical analysis was subsequently performed using SPSS Statistics 17.0 (IBM, USA) and Origin Pro 9.0 (OriginLab Corporation, USA).

Dorst M.C., Tokarska A., Zhou M., Lee K., Stagkourakis S., Broberger C., Masmanidis S, & Silberberg G. (2020). Polysynaptic inhibition between striatal cholinergic interneurons shapes their network activity patterns in a dopamine-dependent manner. Nature Communications, 11, 5113.

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Quantitative Bioimaging of Platinum in Tissues

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For LA-ICP-MS measurements, tumor and kidney samples were embedded in Tissue-Tek medium and cryosectioned into slices of 20 μm thickness with a cryotom (Microm HM 550, Thermo Fisher). Quantitative bioimaging by LA-ICP-MS was performed according to a previously described procedure, using matrix-matched calibration standards.^{29 (link)} A Nd:YAG solid state laser (NWR 213, ESI, Fremont, CA, USA) at a wavelength of 213 nm was used to obtain the spatially-resolved distribution of platinum in tumor and kidney sections. Laser ablation was performed as described previously.^{9 (link)} Data were recorded by using a Triple Quadrupole ICP-MS Agilent 8800 instrument (Agilent Technologies, Tokyo, Japan) and processed with the Agilent MassHunter software package (Workstation Software, Version B.01.03, 2013). The software Igor Pro (Wavemetrics, Igor Pro 6.34A) together with its add-on Iolite (Iolite Version 2.5) was used for further data processing and generation of platinum distribution maps.^{30 (link)}

Legin A.A., Theiner S., Schintlmeister A., Reipert S., Heffeter P., Jakupec M.A., Mayr J., Varbanov H.P., Kowol C.R., Galanski M.S., Berger W., Wagner M, & Keppler B.K. (2016). Multi-scale imaging of anticancer platinum(iv) compounds in murine tumor and kidney. Chemical Science, 7(5), 3052-3061.

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Kinetic Analysis of Acetyl-CoA and Tryptamine

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To differentiate between sequential and classic ping-pong kinetic mechanisms, double-reciprocal plots of the initial velocity data were generated for acetyl-CoA and tryptamine in SigmaPlot 12.0. Non-linear regression analysis of initial rates and model discrimination analyses were performed in WaveMetrics IGOR Pro 6.34A. Initial velocities were determined by varying the concentration of one substrate, while holding the other substrate at a fixed concentration. The two plots were generated by holding the tryptamine concentration constant (40 μM, 60 μM, 120 μM, 250 μM, and 500 μM) and varying the concentration of acetyl-CoA. Accordingly, the second plot held the concentration of acetyl-CoA constant (15 μM, 25 μM, 40 μM, 90 μM, and 200 μM) and varied the tryptamine concentration. The resulting initial velocity data was fit to Equation 3 for an ordered bi-bi mechanism and to Equation 4 for ping-pong mechanism, where v_o is the initial velocity, V_max is the maximal velocity, [A] is the concentration of substrate A, [B] is the concentration of substrate B, K_ia is the dissociation constant for substrate A, K_b is the Michaelis constant for substrate B, and K_a is the Michaelis constant for substrate A. K_ia, the dissociation constant for acetyl-CoA, was fixed at 0.7 μM, based on the direct measurement of acetyl-CoA binding to Bm-iAANAT3 by isothermal calorimetry (ITC) [34 (link)].

Battistini M.R., O’Flynn B.G., Shoji C., Suarez G., Galloway L.C, & Merkler D.J. (2018). Bm-iAANAT3: Expression and Characterization of a Novel Arylalkylamine N-acyltransferase from Bombyx mori. Archives of biochemistry and biophysics, 661, 107-116.

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Whole-cell Patch-clamp of HEK293T Cells

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Whole-cell patch clamp recordings from transiently transfected HEK293T cells were performed at room temperature (22–24°C) and with heat (up to ~50°C) followed. Current responses were low-pass filtered at 2 kHz (Axopatch 200B), digitally sampled at 5–10 kHz (Digidata 1440A), converted to digital files in Clampex10.7 (Molecular Devices) and stored on an external hard drive for offline analyses (Clampfit10.7, Molecular Devices; Excel 2010, Microsoft Office; Igor Pro 6.34A, Wavemetrics). Pipettes were pulled from borosilicate glass and heat-polished to final resistances between 3 and 7 MΩ. Electrodes were filled with an intracellular solution containing (in mM) 140 NaCl, 5 MgCl₂, 10 HEPES, 5 EGTA, and adjusted to pH 7.4 (NaOH). MgCl₂ was included to both increase the quality of the seals and to block endogenous HEK293T channels. The extracellular solution consisted of (in mM): 140 NaCl, 10 HEPES, 5 EDTA, pH 7.4 (NaOH). For heat activation, we used the same method as described in oocyte temperature recordings above.

Kwon D.H., Zhang F., Suo Y., Bouvette J., Borgnia M.J, & Lee S.Y. (2021). Heat-dependent opening of TRPV1 in the presence of capsaicin. Nature structural & molecular biology, 28(7), 554-563.

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TRPV4 Whole-Cell Patch-Clamp Analysis

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After 1 day co-expression of GFP and wild type TRPV4 and its mutants, the whole-cell configuration patch clamp recordings were done at room temperature (22 ˚C). Data were acquired with an Axopatch 200B amplifier (Molecular Devices), currents were low-pass filtered at 2 kHz (Axopatch 200B) and digitally sampled at 5–10 kHz (Digidata 1440 A). Pipettes were pulled from borosilicate glass (1.5 mm O.D. x 0.86 mm I.D. x 75 mm L; Harvard Apparatus) using a Sutter P-97 puller and heat-polished to final resistances between 2 and 3 MΩ. 90% series resistance (Rs) compensation was used in all whole-cell recordings. Electrodes were filled with an intracellular solution containing 140 mM NaCl, 1 mM MgCl₂, 10 mM HEPES, and adjusted to pH 7.4 (NaOH). The extracellular solution contained 140 mM NaCl, 10 mM HEPES, 5 mM EGTA, pH 7.4 (NaOH), GSK101 and ruthenium red (RR) were applied using a gravity-fed perfusion system. Currents were recorded using a voltage ramp protocol consisting of 50 ms at a holding potential of −60 mV, 1000 ms ramp to +60 mV, followed by another 50 ms at 60 mV. All electrophysiological data analysis was done using Igor Pro 6.34 A (Wavemetrics).

Kwon D.H., Zhang F., McCray B.A., Feng S., Kumar M., Sullivan J.M., Im W., Sumner C.J, & Lee S.Y. (2023). TRPV4-Rho GTPase complex structures reveal mechanisms of gating and disease. Nature Communications, 14, 3732.

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Acetyl-CoA Kinetics: pH Dependence

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The pH dependence of the kinetic constants for acetyl-CoA (in the presence of saturating 8.0 mM tryptamine) were determined at intervals of 0.5 pH units ranging from pH 6.0 to 9.5. The buffers used were the following: MES (pH 6.0 – 6.5), Tris (pH 7.0 – 9.0) and AmeP (pH 9.0 – 9.5). The resulting kinetic data was fit to Equation 8 in WaveMetrics IGOR Pro 6.34A to delineate the pKa values of measured ionizable groups. In this equation, y represents either k_cat/K_m or K_m, c is the pH-independent plateau, H is the hydrogen ion concentration, and K_a represents the two unresolvable hydrogen ion dissociation constants.

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Corrosion Monitoring via AFM Imaging

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Atomic force microscopy images were recorded for Zn/Chit and Zn/Chit-AcAMT samples before and after 3 days of exposure to a 0.2 g/L Na2SO4 corrosive solution.
High-resolution topographies were recorded with an MFP-3D atomic force microscope, (Asylum Research, Santa Barbara CA; driving software written in IgorPro 6.34A, Wavemetrics).
Rectangular silicon cantilevers with a tetrahedral tip with a radius below 10 nm were used in these measurements (AC240, Olympus, Optical Co. Ltd. Tokyo, Japan). Prior to measurements, the spring constant for each cantilever was calibrated, according to standard built-in procedures [30, (link)31] (link), prior to measurements. The images were recorded with a typical resolution of 512 by 512 pixels, with a scan speed of 10 µm/s in a closed loop. In order to correct potential sample tilt, all height images were first-order flattened and planefitted.

Szőke Á.F., Szabó G.S., Hórvölgyi Z., Albert E., Végh A.G., Zimányi L, & Muresan L.M. (2020). Accumulation of 2-Acetylamino-5-mercapto-1,3,4-thiadiazole in chitosan coatings for improved anticorrosive effect on zinc. International journal of biological macromolecules, 142.

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