Il 18 sc 7954

Following antibodies were used to specifically detect proteins in immunoblotting: AR (sc-816), IFI16 (sc-8023), ASC (sc-22514), IL-1β (sc-7884), and IL-18 (sc-7954) from Santa Cruz Biotech (Santa Cruz, CA, USA); Caspase-1 (AHZ0082) from Invitrogen (Grand Island, NY, USA); Anti-STAT1 (cat # 9172), p-STAT1 (cat # 9171), and β-actin (cat # 4967) from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal antibodies that we raised against the C-terminal AIM2 peptide that specifically detected two human hAIM2 isoforms have been described [31 (link)]. Specific custom anti-peptide rabbit polyclonal antibodies were raised against a peptide (REEQETGICGSPSSARSV) in the POP3 protein, which detected an IFN-inducible POP3 protein of an expected size (~18 kDa) in total cell extracts from IFN-treated THP-1 cells as described [15 (link)]. Horseradish peroxidase (HRP) conjugated secondary anti-mouse (NXA-931) and anti-rabbit (NA-934) antibodies were from GE Healthcare Biosciences (Piscataway, NJ, USA).

Following specific antibodies were purchased to detect proteins in immunoblotting: ASC (sc-22514), IL-1β (sc-7884), and IL-18 (sc-7954) from Santa Cruz Biotech (Santa Cruz, CA); NLRP3 (HPA012878) from Sigma-Aldrich; Caspase-1 (AHZ0082) from Invitrogen (Grand Island, NY); β-actin (cat # 4967), IκBα (# 9247); and histone 3 (# 9715) from Cell Signaling Technology (Danvers, MA). Rabbit polyclonal antibodies to specifically detect two human hAIM2 isoforms that were raised by us have been described [29 (link)]. Horseradish peroxidase (HRP) conjugated secondary anti-mouse (NXA-931) and anti-rabbit (NA-934) antibodies were from GE Healthcare Biosciences (Piscataway, NJ).