Agilent two-color human gene expression microarrays were processed using limma [30 (link)]. Fold changes were obtained by calculating the log-ratio between the intensities of the red channel (corresponding to experimental samples) and the green channel (corresponding to Human Universal Reference RNA [31 (link)]). These were later background-corrected and normalized using the LOESS algorithm. Agilent one-color mouse gene expression microarrays were processed in a similar manner as the two-color arrays, but using the reported expression value for each probe instead of the log-ratio between the two channels. Benjamini-Hochberg was used to correct the false discovery rate of multiple testing.
Two color human gene expression microarrays
Manufactured by Agilent Technologies
Two-color human gene expression microarrays are a type of lab equipment used to measure the expression levels of thousands of human genes simultaneously. They provide a comprehensive view of gene activity within a sample.
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Lab products found in correlation
2 protocols using two color human gene expression microarrays
1
Gene Expression Microarray Analysis
2
Human Gene Expression Microarray Analysis
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Agilent two-color human gene expression microarrays were processed using limma [53 (link)]. Fold changes were obtained by calculating the log-ratio between the intensities of the red channel (which corresponds to experimental samples) and the green channel (which corresponds to Human Universal Reference RNA [54 (link)]). These were later background-corrected and normalized using LOESS [55 (link)].
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