Human glioblastoma-astrocytoma U-87-MG (NCI-PBCF-HTB14; ATCC HTB-14) and human brain malignant glioma GBM 8401 cells were obtained from Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan) [21 (link)]. All cell lines were incubated in an atmosphere containing 5% CO2 at 37°C. GBM8401 cells were cultured in a RPMI1640 medium with supplemental 10% fetal bovine serum (FBS) and U87 MG cells in modified Eagle's Medium (MEM) with supplemental 10% FBS.
Gbm8401
Manufactured by RIKEN BioResource Center
Sourced in United States
The GBM8401 is a laboratory equipment designed for cell culture applications. It is a multi-functional incubator that provides a controlled environment for the growth and maintenance of cell lines. The GBM8401 is capable of maintaining precise temperature, humidity, and atmospheric conditions to support optimal cell culture conditions.
Automatically generated - may contain errors
Lab products found in correlation
Gbm8901, by RIKEN BioResource Center (2 mentions)
Dbtrg 05mg, by RIKEN BioResource Center (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Pgl4.51 luc2 cmv neo vector, by Promega (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Lipofetamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
U87mg, by American Type Culture Collection (1 mentions)
U87mg, by RIKEN BioResource Center (1 mentions)
Htb 14, by American Type Culture Collection (1 mentions)
11 protocols using gbm8401
1
Culturing Glioma Cell Lines
2
GBM Cell Line Establishment and Culture
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GBM8401 and A172 were the GBM cell lines. GBM8401 was obtained from the Bioresource Collection and Research Center (BCRC, Taipei, Taiwan; 60163), and A172 was obtained from the American Type Culture Collection (ATCC, Manassas, VG, USA; CRL-1620). GBM8401 cells were cultured in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% fetal bovine serum (FBS). A172 cells were cultured in DMEM supplemented with 10% FBS. All cells were incubated at 37 °C with 5% CO2.
3
Glioblastoma Cell Lines and Primary Cultures
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Glioblastoma U87, C6, and Hs683 cells were obtained from American Type Culture Collection and GBM8401 was obtained from Bioresource Collection and Research Center of Taiwan. Those cell lines were grown in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Biological Industries). Primary cortical neuron and astrocyte cultures were prepared as previously described [44 (link)]. The human glioma cell lines were authenticated through cell morphology monitoring, growth curve analysis and short tandem repeat profiling analysis in 2014. The characterization of primary cortical neuron, primary astrocyte and C6 glioma cell line were confirmed by NeuN, GFAP or S100 expression, respectively. For the intracranial xenograft experiment, luciferase-expressing U87 and GBM8401 cells were established by transfection of a pGL4.51[luc2/CMV/Neo] vector (Promega) into the cell lines.
4
Glioma Cell Line Cultivation
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Human glioma cell lines, GBM8401, GBM8901, and DBTRG-05MG were purchased from Bioresource Collection and Research Center in the year 2014 (Hsinchu, Taiwan). Human glioma cell lines, A172, LN18, U118, and U251-MG, and mouse glioma cells, GL261, were kindly provided by Dr. Wei KC (Chang Gung Memorial Hospital). Cells were cultured in DMEM containing 10% FBS in 5% CO2 at 37°C.
5
Knockdown of SRSF1 and SEDT2 in Human Cells
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A human fibroblast cell line (established from normal human skin, 27 years old, male, Asian) was kindly provided by Dr. Chung-Hsing Chang (School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan), and the A549, GBM8401, and Huh-7 cell lines were obtained from the Bioresource Collection and Research Center. Cells were maintained in Dulbecco’s Modifed Eagle’s medium that was supplemented with 10% fetal bovine serum and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) at 37 °C in a humidified atmosphere of 5% CO2. To perform SRSF1 or SEDT2 knockdown, antisense oligonucleotide sequences of SRSF1, SEDT2, or a scrambled control (Supplementary Table S1 ) were transfected into cells using Lipofetamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol.
6
Cultured Cell Lines for Glioblastoma Research
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The human glioblastoma cell line, GBM8401, was acquired from the Bioresource Collection and Research Center (BCRC), while U251 was procured from Sigma-Aldrich. The U251 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, and 1% penicillin-streptomycin (P/S). The GBM8401 cells were maintained in RPMI 1640 (Invitrogen) supplemented with 10% FBS, 10 mM HEPES, and 1% P/S. Primary glioblastoma cells, GBM04T and GBM09T, derived from our previous study [22 (link)], were propagated as tumor spheres in serum-free DMEM-F12 supplemented with 2% B27, 20 ng/mL bFGF, and 20 ng/mL EGF. The human umbilical vein endothelial cells (HUVECs) were procured from Sciencell Company and cultured with endothelial cell medium (ECM) (Sigma-Aldrich) containing 5% FBS, 1% endothelial cell growth supplement (ECGS), and 1% P/S.
7
Glioblastoma Cell Lines and THD Analogs
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The human glioblastoma U87MG (Cat# HTB-14, p53-WT, MGMT negative) cell line was obtained from the American Type Culture Collection (Manassas, VA, USA). GBM 8401 (Cat# 60163, p53-mutated, MGMT negative) was obtained from the Bioresource Collection and Research Center (Hsinchu, Taiwan). Cells were cultured in minimum essential medium (Gibco; Thermo Fischer Scientific, Grand Island, NY, USA) and RPMI-1640 (Gibco) supplemented with 10% fetal bovine serum (Gibco), 2 mM L-glutamine (Gibco), and antibiotics (penicillin/streptomycin, 100 IU/L; Gibco). THD (Sigma, Aldrich, St. Louis, MO, USA) and its analogs were prepared in our lab.
8
Cultivation of Glioblastoma Cell Lines
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Human glioblastoma‐astrocytoma U‐87‐MG (NCI‐PBCF‐HTB14; ATCC HTB‐14) and human brain malignant glioma GBM 8401 cells were obtained from Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan). GBM 8401 cells were maintained in 90% (v/v) RPMI 1640 and 10% (v/v) FBS with 2 mmol/L l ‐glutamine and 1.5 g/L sodium bicarbonate, and U‐87‐MG cells were maintained in DMEM with 10% (v/v) FBS with 2 mmol/L l ‐glutamine, 1.5 g/L sodium bicarbonate and 0.1 mmol/L non‐essential amino acid (NEAA). These cells were incubated in a humidified atmosphere of 5% CO2 and 95% air at 37°C.
9
Glioblastoma Cell Line Characterization
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All the cells were incubated in 5% CO2 at 37 °C. SVGp12, U87MG, GBM8401, GBM8901, DBTRG-05MG, G5T/VGH, and M059K were obtained from the Bioresource Collection and Research Center (BCRC), whereas A172 was obtained from the American Type Culture Collection. GBM8401, GBM8901, and DBTRG-05MG cells were cultured in 90% Roswell Park Memorial Institute medium supplemented with 10% foetal bovine serum (FBS). SVGp12 and U87MG cell lines were cultured in minimum essential medium containing 10% FBS. The G5T and A172 cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS. M059K cells were grown in DMEM-F12 supplemented with 10% FBS. The SVGp12 cell line was isolated from normal glial cells and was used as a normal control.
10
Comparative Study of Glioblastoma Cell Lines
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Two different glioblastoma cancer cell lines, human astrocytoma U87MG (NCI-PBCF-HTB14; ATCC HTB-14) and human glioblastoma GBM8401 cells differ in the degree of malignancy. And both cell lines were purchased from Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan). GBM8401 cells were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C in a 5% CO2 atmosphere. U87MG cells were cultured in modified Eagle’s medium (MEM) at 37 °C in a 5% CO2 atmosphere supplemented with 10% FBS and 1% penicillin/streptomycin.
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