Log In Sign up
The largest database of trusted experimental protocols

Anti ddx21

Manufactured by Novus Biologicals
Visit Supplier

Anti-DDX21 is a polyclonal antibody that recognizes the DDX21 protein. DDX21 is an RNA helicase involved in the regulation of ribosomal RNA transcription and processing. This antibody can be used for the detection of DDX21 in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Anti flag, by Merck Group (3 mentions) Anti sting, by Cell Signaling Technology (1 mentions) Anti ha hrp, by Merck Group (1 mentions) Anti p65, by Cell Signaling Technology (1 mentions) Poly 1 c, by InvivoGen (1 mentions) Anti phospho p65, by Cell Signaling Technology (1 mentions) Anti phospho irf3, by Cell Signaling Technology (1 mentions) Biotin labeled poly 1 c, by InvivoGen (1 mentions) Anti irf3, by Novus Biologicals (1 mentions) Anti gapdh hrp, by Merck Group (1 mentions) Anti myc hrp, by Merck Group (1 mentions) Anti myc beads, by Merck Group (1 mentions) Poly dg dc, by InvivoGen (1 mentions) Anti mavs, by Cell Signaling Technology (1 mentions) Anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Anti erk1 2, by Cell Signaling Technology (1 mentions) Anti flag hrp, by Merck Group (1 mentions) Anti p38, by Cell Signaling Technology (1 mentions) Anti phospho p38, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

4 protocols using anti ddx21

1

Investigating Viral RNA Sensing Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sendai virus was purchased from The American Type Culture Collection (ATCC, Manassas, VA). Reovirus was purchased from Advanced Biotechnologies, Inc. (Columbia, MD). Poly I:C, poly dG:dC and biotin-labeled Poly I:C were purchased from InvivoGen (San Diego, CA). Lipofectamine™ 2000 was purchased from Invitrogen (Carlsbad, CA). DHX15 antibody was purchased from Abcam (Cambridge, MA) and used for immunoprecipitation and immunoblotting. The following antibodies were used for immunoblotting: anti-IRF3 (Santa Cruz, Dallas, TX); anti-DDX21 (Novus Biologicals, Littleton, CO); anti-MAVS, anti-STING, anti-Erk1/2, anti-p38, anti-p65, anti-phospho-Erk1/2, anti-phospho-p38, anti-phospho-p65 and anti-phospho-IRF3 (Cell Signaling, Danvers, MA); anti-DHX41, anti-GAPDH-HRP, anti-Flag-HRP, Anti-HA-HRP and anti-Myc-HRP (Sigma, St. Louis, MO). Anti-HA and anti-Myc beads were purchased from Sigma. Protein A/G beads and NeutAvidin beads were purchased from Thermo Scientific (Rockford, IL).
Lu H., Lu N., Weng L., Yuan B., Liu Y.J, & Zhang Z. (2014). DEAH (Asp-Glu-Ala-His) box helicase 15 senses double-stranded RNA in myeloid dendritic cells. Journal of immunology (Baltimore, Md. : 1950), 193(3), 1364-1372.
+ Open protocol
+ Expand
2

Preparation and Analysis of HEK293 Nuclear Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293 nuclear extracts were prepared as described previously35 (link). For immunoprecipitations, extracts were incubated overnight with 3 μg of the desired antibody pre-bound to protein G-sepharose (Pierce). In some case protein extracts were treated with RNaseA (20 μg ml−1). Immunocomplexes were eluted in 2× Laemmli buffer and resolved in an 8% acrylamide gel. For western blots the following antibodies were used according to manufacturer instructions: anti-NOP58 (Bethyl A302-718A); anti-fibrillarin (Cell Signaling C13C3); anti-DKC1 (Gene Tex GTX109000); anti-Flag (Sigma); anti-DDX21 (Novus Biologicals NB100-1781); anti-LARP7 (a gift from D. H. Price); anti-CDK9 (Santa Cruz Biotechnology sc-484); anti-cyclinT1 (Santa Cruz Biotechnology sc-10750); and anti-HEXIM1 (Bethyl A303-113A). All antibodies have been previously validated unless otherwise specified.
Calo E., Flynn R.A., Martin L., Spitale R.C., Chang H.Y, & Wysocka J. (2014). RNA helicase DDX21 coordinates transcription and ribosomal RNA processing. Nature, 518(7538), 249-253.
+ Open protocol
+ Expand
3

Plasmid and Antibody Characterization

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasmids encoding Flag-hSIRT7, Flag-PCAF, CBP-HA, and p300-HA have been described (Muth et al. 2001 (link); Ford et al. 2006 (link)). cDNAs of DDX21and mutants DDX21SAT, DDX21DEV, and DDX213KQ were generated by PCR and cloned into pCMV-Tag2. Antibodies against SIRT7 (Chen et al. 2016 (link)), RPA194 (Percipalle et al. 2006 (link)), and RPA116 (Seither et al. 1997 (link)) have been reported. The S9.6 antibody was purified from the hybridoma HB-8739 cell line (Boguslawski et al. 1986 (link)). Commercial antibodies used were anti-acetyl-lysine (Cell Signaling Technology, 9441), anti-DDX21 (Novus Biologicals, NB100-1718), anti-Flag (Sigma, F3165), anti-GFP (Abcam, ab290), anti-pSer5-Pol II (Abcam, ab5408), anti-pSer2-Pol II (Millipore, MABE953), anti-tubulin (Sigma, clone B-5-1-2, T6074), anti-γH2AX (Millipore, 05-636), and anti-H2A (Millipore, 07-146). anti-Flag (M2) beads (Sigma, A220) were used for precipitation of Flag-tagged proteins, and the GFP-Trap (Chromotek, gta) was used for purification of GFP-tagged proteins. Anti-rabbit or anti-mouse secondary antibodies conjugated to HRP were from Dianova (111-035-144 and 115-035-062).
Song C., Hotz-Wagenblatt A., Voit R, & Grummt I. (2017). SIRT7 and the DEAD-box helicase DDX21 cooperate to resolve genomic R loops and safeguard genome stability. Genes & Development, 31(13), 1370-1381.
+ Open protocol
+ Expand
4

Quantitative Nuclear Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nuclear extracts were prepared according to the Dignam and Roeder protocol32 . For immunoprecipitations, extracts were incubated overnight with 3–5 μg of the desired antibody pre-bound to Protein G Sepharose (Pierce). In some cases, protein extracts were treated with RNaseA (20 μg ml−1). Immunocomplexes were eluted in 2× Laemmli buffer and resolved in a 4–20% pre-casted tris-glycine gel (Life Technologies). For western blots, the following antibodies were used according to the manufacturer’s instructions: anti-GFP (Thermo Fisher Scientific A-6455), anti-TCOF1 (Novus Biologicals NBP1-86909 and Abnova H00006949-B01P), anti-Flag (Sigma-Aldrich), anti-DDX21 (Novus Biologicals NB100-1781), anti-p53 (Vector Laboratories VP-P953), anti-ACTIN (Abcam ab49900). All antibodies had been previously validated unless otherwise specified.
Calo E., Gu B., Bowen M.E., Aryan F., Zalc A., Liang J., Flynn R.A., Swigut T., Chang H.Y., Attardi L.D, & Wysocka J. (2018). Tissue–selective effects of nucleolar stress and rDNA damage in developmental disorders. Nature, 554(7690), 112-117.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!