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2 protocols using eugenol 4 allyl 2 methoxyphenol

1

Biofunctionalized Silica Nanoparticle Synthesis

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Cetyltrimethylammonium bromide (CTAB), tetraethyl orthosilicate (TEOS, 99.98%), (3-aminopropyl) triethoxysilane (APTES), and eugenol (4-allyl-2-methoxyphenol) were purchased from Sigma-Aldrich. Casein, trypsin solution, absolute ethanol (EtOH), succinic anhydride, 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (sulfo-NHS), N,N-dimethylformamide (DMF), 2-(N-morpholino)ethanesulfonic acid (MES) buffer, and glacial acetic acid were purchased from ThermoFisher Scientific, USA. Chemical reagents were of analytical grade and were used as received.
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2

Functionalized Silica Nanoparticle Synthesis

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Cetyltrimethylammonium bromide (CTAB), tetraethyl orthosilicate (TEOS, 99.98%), 3-aminopropyltriethoxysilane (APTES), fluorescein isothiocyanate (FITC), and eugenol (4-allyl-2-methoxyphenol) were purchased from Sigma-Aldrich. BSA, casein, trypsin solution, absolute ethanol (EtOH), succinic anhydride, 1-ethyl-3-(3-(dimethylamino)-propyl)carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (sulfo-NHS), N,N-dimethylformamide (DMF), 2-(N-morpholino)ethanesulfonic acid (MES buffer), and glacial acetic acid were purchased from Fisher Scientific. The reagents used in this study are of analytical grade and used as received.
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