Sporophylls of the brown algae U. pinnatifida were obtained from the seaweed-processing factory in Wando, South of Korea. Impurities were removed using tap water, and the samples were subjected to freeze-drying and stored at −40 °C. Trifluoroacetic acid (TFA) of analytical grade (Dae-Jung Chemicals & Metals Co., Seoul, Korea) was used for the monosaccharide compositional analysis and 97% ethanol (Dae-Jung Chemicals & Metals Co., Seoul, Korea) was used for extraction. Celluclast was purchased from Novo Nordisk (Bagsvaerd, Denmark). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), and AAPH were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and reagents used in these experiments were of analytical grade.
Celluclast
Manufactured by Novo Nordisk
Sourced in Denmark
Celluclast is a laboratory enzyme product manufactured by Novo Nordisk. It is a cellulase enzyme preparation derived from the fungus Trichoderma reesei. Celluclast is used for the hydrolysis of cellulosic materials.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt dimethyl sulfoxide dmso, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Daejung Chemicals (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Alcalase, by Novo Nordisk (1 mentions)
Viscozyme, by Novo Nordisk (1 mentions)
Raw 264, by Korean Cell Line Bank (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Sodium azide, by Merck Group (1 mentions)
3 protocols using celluclast
1
Monosaccharide Analysis of Sporophylls from Undaria pinnatifida
2
Macrophage Cell Line Characterization
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The murine macrophage cell line RAW 264.7 was purchased from the Korean Cell Line Bank (KCLB; Seoul, Korea). Dulbecco's modified Eagle's medium (DMEM), penicillin-streptomycin, and fetal bovine serum (FBS) were purchased from Gibco BRL (Burlington, ON, Canada). 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), fucoidan (Product No. F5631-1G), and FT-IR-grade KBR powder were purchased from Sigma-Aldrich (St. Louis, MO, USA). Three carbohydrate-degrading enzymes (AMG, Celluclast, and Viscozyme) and one protease (Alcalase) were donated by Novo Nordisk (Bagsvaerd, Denmark). Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α, IL-1β and PGE2 were purchased from R&D Systems Inc. (Minneapolis, MN, USA). All other chemicals and reagents used in these experiments were of analytical grade.
3
Enzymatic Isolation of Fruit Cuticles
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Cuticular membranes of fruits were enzymatically isolated with 1% (v/v) pectinase (Trenolin SuperPlus, Erbslöh), and 1% (v/v) cellulase (Celluclast, Novo Nordisk) in 20 mM citric acid (Roth), pH 3.0, containing 1 mM sodium azide (Sigma-Aldrich) at room temperature. The enzyme solution was exchanged weekly. Isolated cuticular membranes were extensively washed in deionized water and air-dried.
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