UV–vis absorption spectra were acquired on a Cary 60 UV–visible spectrometer (Agilent). HPLC-coupled HR-MS and HR-MS/MS were carried out on an Agilent 6546 accuratemass quadrupole time-of-flight (Qtof) MS instrument, equipped with an automated liquid sampler, a 1290 Infinity II Series LC system, a diode array detector, a JetStream ESI source, and the 6546 Series Qtof. Compounds were separated on an Aeris 3.6 μm PEPTIDE XB-C18 100 Å LC column (100 mm × 4.6 mm), operating at 0.5 mL/min with a gradient of 10% MeCN in H2O to 50% MeCN over 20 min; all solvents contained 0.1% formic acid (FA). Fast protein liquid chromatography (FPLC) was performed on a Bio-Rad NGC Chromatography System using a HiPrep 16/60 Sephacryl S-200 HR column (60 cm × 16 mm, 50 μm avg. part size) with a flow rate of 0.3 mL/min.
1290 infinity 2 series lc system
Manufactured by Agilent Technologies
Sourced in United States
The 1290 Infinity II Series LC system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. It features a modular design and advanced technologies to deliver precise and reliable separation and detection of a wide range of analytes. The system is capable of operating at high pressures and flow rates, making it suitable for a variety of chromatographic techniques.
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Lab products found in correlation
2 protocols using 1290 infinity 2 series lc system
1
Analysis of Organic Compounds via HPLC-MS
2
Analytical Characterization of Small Molecules and Proteins
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Reactions containing only small molecule components were monitored using an Agilent (US) 1100 series LC/MS (single quadrupole) system equipped with an electrospray interface, a UV diode array detector and an ACE3 C8 (3.0 × 50 mm) column (ACE, UK) with a gradient of acetonitrile in 0.1% aqueous trifluoroacetic acid over 3 min and a flow of 1 mL min -1 .
Upon derivatization, proteins and conjugates were analyzed using an Agilent (US) 1290 Infinity II series LC system equipped with a UV diode array detector and an Agilent (US) 6550 iFunnel q-ToF mass spectrometer. An Acquity UPLC Protein BEH C4 (2.1 × 50 mm) column (Waters, US) and unless otherwise noted, a gradient of 0.1% formic acid in acetonitrile in 0.1% aqueous formic acid over 10 min and a flow of 1 mL min -1 were used.
Preparative HPLC was performed using a Gilson HPLC System (US) equipped with a UV diode array detector and an ACE3 C18-HL (250 × 21.2 mm) column (ACE, UK) with a gradient of acetonitrile in 0.1% aqueous trifluoroacetic acid over 10 min and a flow of 25 mL min -1 .
Please refer to the ESI † for more in-depth general information as well as additional experimental procedures, analytical and biological data.
Upon derivatization, proteins and conjugates were analyzed using an Agilent (US) 1290 Infinity II series LC system equipped with a UV diode array detector and an Agilent (US) 6550 iFunnel q-ToF mass spectrometer. An Acquity UPLC Protein BEH C4 (2.1 × 50 mm) column (Waters, US) and unless otherwise noted, a gradient of 0.1% formic acid in acetonitrile in 0.1% aqueous formic acid over 10 min and a flow of 1 mL min -1 were used.
Preparative HPLC was performed using a Gilson HPLC System (US) equipped with a UV diode array detector and an ACE3 C18-HL (250 × 21.2 mm) column (ACE, UK) with a gradient of acetonitrile in 0.1% aqueous trifluoroacetic acid over 10 min and a flow of 25 mL min -1 .
Please refer to the ESI † for more in-depth general information as well as additional experimental procedures, analytical and biological data.
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