Anti-mouse CD3 (clone PC-61) (QuantoBio), anti-mouse TCRβ chain (clone H75-597) (eBioscience, San Diego, CA, USA), anti-mouse CD4 (clone RM4-5) (eBioscience), anti-mouse CD8a (clone 53-6.7) (eBioscience), rat anti-mouse CD44 (clone IM7) (BD Biosciences, Franklin Lakes, NJ, USA), and rat anti-mouse CD62L (MEL-14) (BD Biosciences) fluorochrome-labeled antibodies were used for flow cytometry. Single cell suspension of spleen and peripheral blood from the mice was prepared as previously described [21 (link)] and stained with the fluorochrome-labeled antibodies, and the fluorescence intensity was analyzed using a FACS Calibur Instrument (BD Biosciences, Franklin Lakes, NJ, USA). FlowJo software version 7.2.5 (TreeStarInt, USA) was used to analyse flow cytometry data.
Anti mouse cd4 clone rm4 5
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-mouse CD4 (clone RM4-5) is a lab equipment product that is used to detect and quantify CD4+ T cells in mouse samples. It is a monoclonal antibody that specifically binds to the CD4 surface marker on mouse T cells.
Automatically generated - may contain errors
2 protocols using anti mouse cd4 clone rm4 5
1
Multiparametric Flow Cytometry Analysis
2
Antibody Depletion Modulation of IFNγ
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On days 5 and 9 PI SeV, 0.1, 1, 10, or 100 µg of anti-mouse IFNγ antibody (clone XMG1.2) or control rat IgG1 antibody (BioLegend) were injected SQ. For NK1.1 depletion, anti-mouse NK1.1 antibody (clone PK136; BioXCell) or control mouse IgG2a (clone C1.18.4; BioXCell) was used, and for CD4 depletion anti-mouse CD4 (clone RM4-5; eBioscience) or control rat IgG2b (eBR2a; eBioscience) were used.
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