Dual-color immunofluorescence was performed to characterize Mo-DC, using the following panel of mAbs: PE- or FITC-conjugated anti-human HLA-DR (clone L242, BD Pharmingen); PE- or FITC-conjugated anti-human CD1a (clone HI149, Biolegend); PE- or FITC-conjugated anti-human CD86 (clone IT2.2, Biolegend); FITC-conjugated anti-human CD40 (clone HB14, Biolegend); PE-conjugated anti-human CD80 (clone 2D10, Biolegend); FITC-conjugated anti-human CD83 (clone HB15e, Biolegend); PE-conjugated anti-human CCR7 (clone 150503, BD Pharmingen). Negative controls were isotype-matched irrelevant mAbs (BD Pharmingen, Biolegend). Cells were analyzed by using FACScan equipment (Becton Dickinson). A minimum of 10000 events was collected in list mode on FACScan software.
Facscan equipment
Manufactured by BD
The FACScan equipment is a flow cytometer designed for cell analysis and sorting. It uses laser technology to detect and measure various physical and fluorescent characteristics of cells or particles in a fluid sample. The FACScan is capable of analyzing multiple parameters simultaneously, providing detailed information about the properties of the analyzed cells or particles.
Automatically generated - may contain errors
Lab products found in correlation
Pe or fitc conjugated anti human hla dr clone l242, by BD (2 mentions)
Pe or fitc conjugated anti human cd1a clone hi149, by BioLegend (2 mentions)
Pe or fitc conjugated anti human cd86 clone it2, by BioLegend (2 mentions)
Fitc conjugated anti human cd40 clone hb14, by BioLegend (2 mentions)
Pe conjugated anti human cd80 clone 2d10, by BioLegend (2 mentions)
Fitc conjugated anti human cd83 clone hb15e, by BioLegend (2 mentions)
Pe conjugated anti human ccr7 clone 150503, by BD (2 mentions)
2 protocols using facscan equipment
1
Phenotypic Characterization of Mo-DC
2
Multicolor Flow Cytometry for Immune Cell Phenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dual-color immunofluorescence was performed using the following panel of mAbs: PE- or FITC-conjugated anti-human HLA-DR (clone L242, BD Pharmingen); PE- or FITC-conjugated anti-human CD1a (clone HI149, Biolegend); PE- or FITC-conjugated anti-human CD86 (clone IT2.2, Biolegend); PE-conjugated anti-human CD80 (clone 2D10, Biolegend); PE- or FITC-conjugated anti-human CD14 (clone HCD14, Biolegend); FITC-conjugated anti-human CD83 (clone HB15e, Biolegend); FITC-conjugated anti-human CD40 (clone HB14, Biolegend); PE-conjugated anti-human CCR7 (clone 150503, BD Pharmingen). Negative controls were isotype-matched irrelevant mAbs (BD Pharmingen, Biolegend). Cells were analyzed by using FACScan equipment or C6 Accuri equipment (Becton Dickinson). A minimum of 10,000 events was collected in list mode on FACScan software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!