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Oil red o isopropanol solution

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Oil-Red O-Isopropanol solution is a staining reagent used in histology and cytology laboratories. It is a solution of the lipophilic dye Oil-Red O dissolved in isopropanol. The solution is used to specifically stain neutral lipids and lipid-rich structures within tissue sections or cell samples.

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Lab products found in correlation

Hematoxylin harris hg free, by Avantor (3 mentions)

Close spelling variants of this product

Oil Red O (7 citations) Oil Red O solution (5 citations)

5 protocols using oil red o isopropanol solution

1

Renal Lipid Deposition Staining

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Filtered Oil-Red O-Isopropanol solution (Electron Microscopy Science, PA) was diluted with water (6:4). 4 μm kidney sections were incubated with 100 µL freshly prepared Oil-Red O solution for 15 min and counterstained with Hematoxylin Harris Hg Free (VWR, PA) to detect lipid deposition. Glomerular staining was evaluated using a light microscope (Olympus BX 41, Tokyo, Japan) [64] (link).
Kim J.J., David J.M., Wilbon S.S., Santos J.V., Patel D.M., Ahmad A., Mitrofanova A., Liu X., Mallela S.K., Ducasa G.M., Ge M., Sloan A.J., Al-Ali H., Boulina M., Mendez A.J., Contreras G.N., Prunotto M., Sohail A., Fridman R., Miner J.H., Merscher S, & Fornoni A. (2020). Discoidin domain receptor 1 activation links extracellular matrix to podocyte lipotoxicity in Alport syndrome. EBioMedicine, 63, 103162.
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2

Detecting Lipid Deposition in Kidney

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Filtered Oil-Red O-Isopropanol solution (Electron Microscopy Science, PA) was diluted with water (6:4). 4μm kidney sections were incubated with 100μl freshly prepared Oil-Red solution for 15 minutes and counterstained with Hematoxylin Harris Hg Free (VWR, PA) to detect lipid deposition. Glomeruli staining was evaluated using a light microscope (Olympus BX 41, Tokyo, Japan)40 (link).
Mitrofanova A., Molina J., Santos J.V., Guzman J., Morales X.A., Ducasa G.M., Bryn J., Sloan A., Volosenco I., Kim J.J., Ge M., Malela S.K., Kretzler M., Eddy S., Martini S., Wahl P., Pastori S., Mendez A.J., Burke G.W., Merscher S, & Fornoni A. (2018). Hydroxypropyl-β-cyclodextrin protects from kidney disease in experimental Alport syndrome and focal segmental glomerulosclerosis. Kidney international, 94(6), 1151-1159.
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3

Histological Analysis of Liver Steatosis

Cited 1 time
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Analyses of serum alanine aminotransferase (ALT) and liver steatosis have been described.(17 (link),18 (link)) For hematoxylin and eosin (H&E) staining, liver tissues were fixed in 10% formalin overnight at 4°C and washed with and stored in 80% ethanol. The fixed tissues were embedded in paraffin, sectioned at 5 μm, and then stained with H&E. For Oil Red O staining, liver tissues were embedded in optimum cutting temperature (OCT) compound, snap frozen, sectioned at 5 μm, and mounted on glass slides, which were fixed in 10% formalin and stained with an Oil Red O iso-propanol solution (Electron Microscopy Sciences, Hatfield, PA).
Han H., He Y., Hu J., Lau R., Lee H, & Ji C. (2017). Disrupted ER‐to‐Golgi trafficking underlies anti‐HIV drugs and alcohol‐induced cellular stress and hepatic injury. Hepatology Communications, 1(2), 122-139.
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4

Histological Analysis of Liver Steatosis

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Analyses of serum alanine aminotransferase (ALT) and liver steatosis have been described.17, 18 For hematoxylin and eosin (H&E) staining, liver tissues were fixed in 10% formalin overnight at 4°C and washed with and stored in 80% ethanol. The fixed tissues were embedded in paraffin, sectioned at 5 μm, and then stained with H&E. For Oil Red O staining, liver tissues were embedded in optimum cutting temperature (OCT) compound, snap frozen, sectioned at 5 μm, and mounted on glass slides, which were fixed in 10% formalin and stained with an Oil Red O isopropanol solution (Electron Microscopy Sciences, Hatfield, PA).
Han H., He Y., Hu J., Lau R., Lee H, & Ji C. (2017). Disrupted ER‐to‐Golgi trafficking underlies anti‐HIV drugs and alcohol‐induced cellular stress and hepatic injury. Hepatology Communications, 1(2), 122-139.
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5

Lipid Quantification in Kidney Cortex

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OCT embedded kidney cortex samples were cryosectioned at 4 μm thickness and stained with Oil-Red O-isopropanol solution (Electron Microscopy Science, PA) diluted 6:4 in water, and then counterstained with Hematoxylin Harris Hg Free (VWR, PA). Sections were then evaluated using a light microscope (Olympus BX 41, Tokyo, Japan).
Wright M.B., Varona Santos J., Kemmer C., Maugeais C., Carralot J.P., Roever S., Molina J., Ducasa G.M., Mitrofanova A., Sloan A., Ahmad A., Pedigo C., Ge M., Pressly J., Barisoni L., Mendez A., Sgrignani J., Cavalli A., Merscher S., Prunotto M, & Fornoni A. (2021). Compounds targeting OSBPL7 increase ABCA1-dependent cholesterol efflux preserving kidney function in two models of kidney disease. Nature Communications, 12, 4662.
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