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Anti flag m2 rabbit mab

Manufactured by Cell Signaling Technology
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The Anti-Flag M2 rabbit monoclonal antibody (MAb) is a laboratory reagent used for the detection and purification of proteins containing the FLAG epitope tag. The antibody specifically recognizes the FLAG peptide sequence (DYKDDDDK) and can be used in various applications such as immunoprecipitation, Western blotting, and affinity chromatography.

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Lab products found in correlation

Horseradish peroxidase hrp conjugated goat anti mouse or anti rabbit igg, by Santa Cruz Biotechnology (4 mentions) Anti gapdh mouse mab, by Santa Cruz Biotechnology (4 mentions) Anti α tubulin mouse mab, by Santa Cruz Biotechnology (4 mentions) Anti his mouse mab, by Beyotime (3 mentions) Anti mouse immunoglobulin g igg, by Beyotime (3 mentions) Anti phospho c met y1356 rabbit polyclonal antibody pab, by Abcam (2 mentions) Anti c met rabbit mab, by Cell Signaling Technology (2 mentions) Anti cd82 rabbit pab, by Santa Cruz Biotechnology (2 mentions) Anti b fgf goat pab, by Santa Cruz Biotechnology (2 mentions) Anti gfp rabbit antibody, by Beyotime (2 mentions) Pf 2341066, by Selleck Chemicals (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Effectence transfection reagent, by Qiagen (2 mentions) Ag490, by Selleck Chemicals (1 mentions) Anti phospho akt ser473 rabbit mab, by Cell Signaling Technology (1 mentions) Anti his rabbit mab, by Cell Signaling Technology (1 mentions) Anti cxcr2 rabbit pab, by Santa Cruz Biotechnology (1 mentions) Anti ha rabbit pab, by Cell Signaling Technology (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Anti phospho akt ser473 mouse mab, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-FLAG Rabbit mAb Anti-FLAG M2 Flag M2 Rabbit anti-Flag Rabbit mABs Anti-Flag

5 protocols using anti flag m2 rabbit mab

1

Inhibition of KSHV-mediated STAT3 Signaling

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Anti-KSHV LANA rat monoclonal antibody (MAb) was purchased from Advanced Biotechnologies Inc.(Columbia, MD, USA) [81 (link)]. Anti-phospho-STAT3 (Y705) rabbit MAb, anti-STAT3 mouse polyclonal antibody (PAb), and anti-Flag M2 rabbit MAb were obtained from Cell Signaling Technologies (Beverly, MA, USA). Anti-SH3BGR mouse MAb, anti-GAPDH mouse MAb, anti-α-Tubulin mouse MAb, and horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit IgG were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-His mouse MAb, anti-mouse immunoglobulin G (IgG) and anti-GST mouse MAb were from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China). Western blotting analysis was performed as described previously [84 (link), 85 (link)]. AG490, a JAK2 inhibitor, was purchased from Selleck Chemicals (Shanghai, China).
Li W., Yan Q., Ding X., Shen C., Hu M., Zhu Y., Qin D., Lu H., Krueger B.J., Renne R., Gao S.J, & Lu C. (2016). The SH3BGR/STAT3 Pathway Regulates Cell Migration and Angiogenesis Induced by a Gammaherpesvirus MicroRNA. PLoS Pathogens, 12(4), e1005605.
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2

Western Blot Analysis of KSHV LANA

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Anti-KSHV LANA ORF73 rat monoclonal antibody (MAb) was purchased from Advanced Biotechnologies Inc. (Columbia, MD, USA) [78 (link)]. Anti-phospho-AKT (Ser473) rabbit MAb, anti-AKT rabbit polyclonal antibody (PAb), anti-Flag M2 rabbit MAb, anti-His rabbit MAb and anti-HA rabbit PAb were obtained from Cell Signaling Technologies (Beverly, MA, USA). Anti-GRK2 mouse MAb, anti-CXCR2 rabbit PAb, anti-GAPDH mouse MAb, anti-α-Tubulin mouse MAb, and horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit IgG were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-GFP mouse MAb was from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China). Western blotting analysis was performed as described previously [83 (link)]. MK-2206, an AKT inhibitor, was purchased from Selleck Chemicals (Shanghai, China).
Hu M., Wang C., Li W., Lu W., Bai Z., Qin D., Yan Q., Zhu J., Krueger B.J., Renne R., Gao S.J, & Lu C. (2015). A KSHV microRNA Directly Targets G Protein-Coupled Receptor Kinase 2 to Promote the Migration and Invasion of Endothelial Cells by Inducing CXCR2 and Activating AKT Signaling. PLoS Pathogens, 11(9), e1005171.
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3

Protein Expression Analysis by Western Blot

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Western blot was performed as previously described (17 (link)). Anti-vascular endothelial growth factor (VEGF) rabbit polyclonal antibody (pAb), anti-glyceraldehyde-3-phosphate dehydrogenase mouse monoclonal antibody (mAb), anti-α-Tubulin mouse mAb, and horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit IgG were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA, USA). Anti-Flag M2 rabbit mAb, anti-phospho-PTEN (Ser380) rabbit pAb, anti-PTEN rabbit pAb, anti-phospho-phosphatidylinositol 3-kinase (PI3K) [p85(Tyr458)/p55(Tyr199)] rabbit pAb, anti-PI3K rabbit pAb, anti-phospho-AKT (Ser473) mouse mAb, anti-AKT mouse mAb, anti-phospho-mTOR (Ser2448) rabbit pAb and anti-His mouse mAb were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-smooth muscle actin (SMA) rabbit pAb was purchased from Abbiotec™ (San Diego, CA, USA). Anti-green fluorescent protein (GFP) mouse mAb was purchased from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China).
Xue M., Yao S., Hu M., Li W., Hao T., Zhou F., Zhu X., Lu H., Qin D., Yan Q., Zhu J., Gao S.J, & Lu C. (2014). HIV-1 Nef and KSHV oncogene K1 synergistically promote angiogenesis by inducing cellular miR-718 to regulate the PTEN/AKT/mTOR signaling pathway. Nucleic Acids Research, 42(15), 9862-9879.
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4

Western Blot Analysis of KSHV LANA and c-Met

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An anti-KSHV LANA rat monoclonal antibody (MAb) was purchased from Advanced Biotechnologies Inc. (Columbia, MD, USA)89 (link). The anti-phospho-c-Met (Y1356) rabbit polyclonal antibody (PAb) was obtained from Abcam (Cambridge, MA, USA). The anti-c-Met rabbit MAb and anti-Flag M2 rabbit MAb were obtained from Cell Signaling Technologies (Beverly, MA, USA). The anti-CD82 rabbit PAb, anti-b-FGF goat PAb, anti-GAPDH mouse MAb, anti-α-Tubulin mouse MAb, and horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit IgG were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-His mouse MAb, anti-GFP rabbit antibody, and anti-mouse immunoglobulin G (IgG) were from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China). Western-blotting analysis was performed as previously described90 (link), 91 (link). Transfection of HUVECs was performed with the Effectence transfection reagent (Qiagen, Valencia, CA, USA) while transfections in other cells were performed with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. PF-2341066, a c-Met inhibitor, was purchased from Selleck Chemicals (Shanghai, China).
Li W., Hu M., Wang C., Lu H., Chen F., Xu J., Shang Y., Wang F., Qin J., Yan Q., Krueger B.J., Renne R., Gao S.J, & Lu C. (2017). A Viral MicroRNA Down-regulates Metastasis Suppressor CD82 and Induces Cell Invasion and Angiogenesis by Activating the c-Met Signaling. Oncogene, 36(38), 5407-5420.
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5

Western Blot Analysis of KSHV LANA and c-Met

Check if the same lab product or an alternative is used in the 5 most similar protocols
An anti-KSHV LANA rat monoclonal antibody (MAb) was purchased from Advanced Biotechnologies Inc. (Columbia, MD, USA)89 (link). The anti-phospho-c-Met (Y1356) rabbit polyclonal antibody (PAb) was obtained from Abcam (Cambridge, MA, USA). The anti-c-Met rabbit MAb and anti-Flag M2 rabbit MAb were obtained from Cell Signaling Technologies (Beverly, MA, USA). The anti-CD82 rabbit PAb, anti-b-FGF goat PAb, anti-GAPDH mouse MAb, anti-α-Tubulin mouse MAb, and horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit IgG were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-His mouse MAb, anti-GFP rabbit antibody, and anti-mouse immunoglobulin G (IgG) were from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China). Western-blotting analysis was performed as previously described90 (link), 91 (link). Transfection of HUVECs was performed with the Effectence transfection reagent (Qiagen, Valencia, CA, USA) while transfections in other cells were performed with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. PF-2341066, a c-Met inhibitor, was purchased from Selleck Chemicals (Shanghai, China).
Li W., Hu M., Wang C., Lu H., Chen F., Xu J., Shang Y., Wang F., Qin J., Yan Q., Krueger B.J., Renne R., Gao S.J, & Lu C. (2017). A Viral MicroRNA Down-regulates Metastasis Suppressor CD82 and Induces Cell Invasion and Angiogenesis by Activating the c-Met Signaling. Oncogene, 36(38), 5407-5420.
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