Aapba

N-Hydroxyethyl acrylamide (HEAA), dopamine hydrochloride, methacryloyl chloride, AAPBA, N,N’-methylenebisacrylamide (MBAA), azobisisobutyronitrile (AIBN), N-chlorosuccinimide (NCS), trifluoroacetic acid (TFA), triethylamine (TEA), methanol, trishydroxymethyl aminomethane (Tris), 3-[4, 5-dimethylthiazoyl-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT), hydrogen chloride (HCl) solution (37 wt%), sodium chloride (NaCl), 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) and dimethyl sulfoxide (DMSO) were all purchased from Sigma-Aldrich. Sodium sulfate (Na₂SO₄), ethyl acetate (EA) and hexane were purchased from Fisher Scientific Co. All the bacteria were purchased from American Type Culture Collection (ATCC). HSF cells were purchased from Honsunbio. Ltd. (Shanghai, China). Lysogeny Broth (LB) agar medium, Mueller Hinton Broth medium (MHB), phosphate buffer saline (PBS), Calcein-AM/PI, LIVE/DEAD Bac Light Bacterial Viability Kit were purchased from Thermo Fisher Scientific. The acidic solution was prepared by titrating a 0.1 M NaCl solution to pH 3 using 1 M HCl, while the basic buffer medium was prepared by titrating 10 mM Tris with 1 M HCl to pH 8.5. Other reagents were used as received without further purification. DMA was prepared using previously published protocol[18 (link)].