Log In Sign up
The largest database of trusted experimental protocols

Ez run prestained protein ladder bp3603

Manufactured by Thermo Fisher Scientific
Visit Supplier

The EZ-Run prestained protein ladder (BP3603) is a ready-to-use molecular weight marker for protein electrophoresis. It contains a mixture of pre-stained proteins with molecular weights ranging from 10 to 250 kDa, which can be used to determine the approximate molecular weights of unknown protein samples.

Automatically generated - may contain errors

Lab products found in correlation

M0630, by Merck Group (2 mentions) Horseradish peroxidase streptavidin sa 5004, by Vector Laboratories (2 mentions) Natural unstained protein ladder 161 0317, by Bio-Rad (2 mentions) B2787, by Bio-Rad (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Thermomixer, by Eppendorf (2 mentions) Biotinyl asp glu val asp aldehyde, by Bachem (2 mentions)

Close spelling variants of this product

EZ-Run protein ladder EZ-Run Protein ladder

2 protocols using ez run prestained protein ladder bp3603

1

Preparation and Analysis of Homocysteinylated Myoglobin

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Horseradish peroxidase streptavidin (SA-5004) was from Vector Laboratories (Burlingame, CA, USA). SuperSignal West Pico chemiluminescent substrate was from Thermo Scientific (Rockford, IL, USA). Myoglobin from equine skeletal muscle (M0630) was from Sigma-Aldrich. N-Homocysteinylated myoglobin was prepared as we previously reported [64 (link)]. Biotinyl-Asp-Glu-Val-Asp-aldehyde (CAS registry number: 178603-73-1) was from Bachem Americas (N-1470, Torrance, CA, USA; see Figure S1 for its structure in Support Information). Rhodamine-aldehyde (10 mM) in 50% 200-proof ethanol was synthesized as previously described [64 (link)]. EZ-Run prestained protein ladder (BP3603) was from Fisher Scientific. Natural unstained protein ladder (161-0317) was from Bio-Rad. Biotinylated protein markers were from Sigma-Aldrich (B2787) and Bio-Rad (161-0319), respectively. All reagents were ACS grade and used as received without further purification. All incubations were carried out in an Eppendorf Thermomixer® (Eppendorf North America, Hauppauge, NY, USA) at 25 °C unless specified otherwise.
Zang T., Pottenplackel L.P., Handy D.E., Loscalzo J., Dai S., Deth R.C., Zhou Z.S, & Ma J. (2016). Comparison of Protein N-Homocysteinylation in Rat Plasma under Elevated Homocysteine Using a Specific Chemical Labeling Method. Molecules, 21(9), 1195.
+ Open protocol
+ Expand
2

Homocysteinylated Myoglobin Labeling Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Horseradish peroxidase streptavidin (SA-5004) was from Vector Laboratories (Burlingame, CA, USA). SuperSignal West Pico chemiluminescent substrate was from Thermo Scientific (Rockford, IL, USA). Myoglobin from equine skeletal muscle (M0630) was from Sigma-Aldrich. N-Homocysteinylated myoglobin was prepared as we previously reported [64 (link)]. Biotinyl-Asp-Glu-Val-Asp-aldehyde (CAS registry number: 178603-73-1) was from Bachem Americas (N-1470, Torrance, CA, USA; see Figure S1 for its structure in Support Information). Rhodamine-aldehyde (10 mM) in 50% 200-proof ethanol was synthesized as previously described [64 (link)]. EZ-Run prestained protein ladder (BP3603) was from Fisher Scientific. Natural unstained protein ladder (161-0317) was from Bio-Rad. Biotinylated protein markers were from Sigma-Aldrich (B2787) and Bio-Rad (161-0319), respectively. All reagents were ACS grade and used as received without further purification. All incubations were carried out in an Eppendorf Thermomixer® (Eppendorf North America, Hauppauge, NY, USA) at 25 °C unless specified otherwise.
Zang T., Pottenplackel L.P., Handy D.E., Loscalzo J., Dai S., Deth R.C., Zhou Z.S, & Ma J. (2016). Comparison of Protein N-Homocysteinylation in Rat Plasma under Elevated Homocysteine Using a Specific Chemical Labeling Method. Molecules, 21(9), 1195.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!