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Fluorescein isothiocyanate conjugated rabbit anti dog igg

Manufactured by Merck Group
Sourced in Italy

Fluorescein isothiocyanate-conjugated rabbit anti-Dog IgG is a laboratory reagent used for the detection and quantification of dog immunoglobulin G (IgG) in various biological samples. The product consists of rabbit-derived antibodies specific to dog IgG that are chemically conjugated with the fluorescent dye fluorescein isothiocyanate (FITC). This conjugation allows for the visualization and tracking of dog IgG in experiments using fluorescence-based methods.

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2 protocols using fluorescein isothiocyanate conjugated rabbit anti dog igg

1

Indirect Fluorescent Antibody Test for Babesia canis

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Dog serum samples were tested with a commercial indirect fluorescent antibody test (IFAT) (MegaScreen®FLUO Babesia canis, MegaCor Diagnostic, Horbranz, Austria) to detect the presence of IgG antibodies against B. canis, using canine erythrocytes infected with B. canis as antigens. Positive and negative controls were always included and were provided by the company. Starting, sera were initially diluted 1:64 in phosphate-buffered saline solution (PBS) (pH = 7.2), applied to slide wells and incubated in a moist chamber for 30 min a 37 °C. The slides were washed and reacted with fluorescein isothiocyanate-conjugated rabbit anti-Dog IgG (Sigma-Aldrich, Milan, Italy), incubated at 37 °C in a humid chamber for 30 min. The slides were mounted with buffered glycerin and covered with a coverslip. Finally, the slides were examined with a microscope (Axioscop 2, Zeiss) under a fluorescent light (HBO50). Those samples that had fluorescence at a diluition ≥ 1:64 were considered as seropositive. Samples resulting seropositive were then diluted to determine the end-point titre (i.e. the last dilution in which is observed positive reaction).
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2

Serological Screening for Borrelia burgdorferi

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Sera from dogs, horses, and humans were screened for anti-B. burgdorferi s.s. antibodies by indirect immunofluorescence antibody test (IFAT) using B. burgdorferi s.s. strain B31 for the antigen. The reaction was performed as previously described (COLLARES-PEREIRA et al., 2000) , with fluorescein isothiocyanate-conjugated rabbit anti-dog IgG (Sigma-Aldrich, St. Louis, MO), rabbit anti-horse IgG (Sigma-Aldrich), and rabbit anti-human IgG (Sigma-Aldrich) used for testing of dog, horse, and human sera, respectively. Samples were considered positive when fluorescence was observed at dilutions of 1:64 or higher in dogs and horses or 1:128 or higher in humans. Antibody titers (endpoint titers) were defined as the reciprocal of the highest dilution of serum in which fluorescence was visualized. Positive and negative controls were provided by the Leptospirosis and Lyme Disease Group at the Universidade Nova de Lisboa, Portugal.
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