Motorized stereotactic apparatus

The mice were placed on a motorized stereotactic apparatus (Stoelting Co., Wood Dale, IL) under anesthesia induced by isoflurane inhalation (induction: 3%; maintenance: 1.5%). The scalp was sterilized with 10% povidone-iodine after the hair was shaved. A 0.5-cm-long middle incision was made to expose the skull and visualize the bregma, which was further set as coordinate zero (x = 0, y = 0, z = 0). Then, a 0.5-cm diameter craniotomy was performed to insert the 2.5-cm-long catheter at the predetermined coordinates (x = −0.34 mm, y = −1.0 mm, z = 0). The incision was closed after the catheter was fixed with acrylic dental cement. The mice were allowed to rest and recover for at least 7 days before undergoing additional procedures.

Mice were placed on the motorized stereotactic apparatus (Stoelting Co., Wood Dale, IL) under anesthetized by inhaling isoflurane (3% induction, 1.5% maintenance). The skin of mice head was cleaned with 10% povidone-iodine after hair shaved. A 0.5-cm-long middle incision was made to expose skull and bregma that was set as coordinate zero (x=0, y=0, z=0). A 5 mm diameter craniotomy was made in predetermined coordinates (x=-0.34 mm, y=-1.0 mm, z=0) to place catheter that inserted into a depth of 2.5 mm. Then, the catheter was fixed by acrylic dental cement, and the incision was closed. Mice were rested for at least 7 days before another operation.