Free floating sections were quenched in 0.3% H2O2 for 25 min, then blocked in 5% goat serum 90 min. They were then incubated with primary antibody mouse anti-parvalbumin (Sigma Aldrich) for 2 days followed by a biotinylated goat anti-mouse antibody (Sigma Aldrich) for 90 min. Following this, solutions A and B from the standard Vectastain ABC kit (Vector Laboratories) 1:500 for 1 h. Staining was developed using a DAB (3,3′-Diaminobenzidine tetrahydrochloride hydrate; Sigma Aldrich) solution. The sections were then mounted onto slides. After air drying, slides were dehydrated in increasing concentration of alcohol, cleared with Hemo-De and coverslipped with Fisher Chemical Permount™ Mounting Medium.
Primary antibody mouse anti parvalbumin
Manufactured by Merck Group
Primary antibody mouse anti-parvalbumin is a laboratory reagent used to detect and identify the presence of the parvalbumin protein in various biological samples. Parvalbumin is a calcium-binding protein found in certain types of neurons and muscle cells. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of parvalbumin in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Hemo de, by Thermo Fisher Scientific (2 mentions)
Biotinylated goat anti mouse antibody, by Merck Group (2 mentions)
3 3 diaminobenzidine tetrahydrochloride hydrate dab, by Merck Group (2 mentions)
Permount mounting medium, by Thermo Fisher Scientific (2 mentions)
Vectastain abc kit, by Vector Laboratories (2 mentions)
2 protocols using primary antibody mouse anti parvalbumin
1
Parvalbumin Immunohistochemistry Protocol
2
Immunohistochemical Staining of Parvalbumin
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Free floating sections were quenched in 0.3% H2O2 for 25 min, then blocked in 5% goat serum 90 min. They were then incubated with primary antibody mouse anti-parvalbumin (Sigma Aldrich) for 2 days followed by a biotinylated goat anti-mouse antibody (Sigma Aldrich) for 90 min. Following this, solutions A and B from the standard Vectastain ABC kit (Vector Laboratories) 1:500 for 1 h. Staining was developed using a DAB (3,3′-Diaminobenzidine tetrahydrochloride hydrate; Sigma Aldrich) solution. The sections were then mounted onto slides. After air drying, slides were dehydrated in increasing concentration of alcohol, cleared with Hemo-De and coverslipped with Fisher Chemical Permount™ Mounting Medium.
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