For osteogenic differentiation, 0.8 × 105 cells suspended in lg-DMEM (ThermoFisher-Gibco) supplemented with 10% FBS were seeded in 35 mm Petri dishes (Corning). When 60% confluence was reached, induction was initiated with StemPro osteogenic medium (Gibco, Carlsbad, California, CA, USA), and the cells were incubated for 21 days, changing the medium twice per week. Finally, alkaline phosphatase activity was detected using SIGMA FAST™ BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) (Sigma-Aldrich).
Stempro osteogenic medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
StemPro osteogenic medium is a cell culture medium designed for the differentiation of human mesenchymal stem cells into osteoblasts. It provides the necessary factors and supplements to support the osteogenic lineage commitment and maturation of stem cells.
Automatically generated - may contain errors
Lab products found in correlation
35 mm petri dishes, by Corning (1 mentions)
Dmem lg, by Thermo Fisher Scientific (1 mentions)
Sigmafast bcip nbt, by Merck Group (1 mentions)
Alizarin red staining, by Merck Group (1 mentions)
Dmem low glucose medium, by Thermo Fisher Scientific (1 mentions)
P35 petri dishes, by Corning (1 mentions)
2 protocols using stempro osteogenic medium
1
Osteogenic Differentiation Protocol
2
Osteogenic Differentiation of hBM-MSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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0.8 × 105 hBM-MSC were seeded in P35 Petri dishes (Corning, New York, NY, USA) with low-glucose DMEM medium (Thermo Fisher, Waltham, MA, USA) supplemented with 10% FBS at 60% confluence. The cells were incubated with StemPro osteogenic medium (Gibco, Carlsbad, CA, USA) for 21 days. The differentiation medium was replaced twice per week. Osteocyte analysis was performed with Alizarin Red staining, which detects calcium deposits (Sigma-Aldrich, St. Louis, MI, USA).
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