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Ab55603

Manufactured by Abcam
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Sourced in United States

Ab55603 is a lab equipment product offered by Abcam. It is a highly specific and sensitive antibody that can be used for the detection of a particular target protein. The core function of this product is to facilitate the identification and analysis of the target protein in research applications.

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Lab products found in correlation

Esgro, by Merck Group (1 mentions) F1804, by Merck Group (1 mentions) Sc 888, by Santa Cruz Biotechnology (1 mentions) Sc 585x, by Santa Cruz Biotechnology (1 mentions) Anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions) Las 3000, by Fujifilm (1 mentions)

2 protocols using ab55603

1

Hoxb1 ChIP-seq in ES cell differentiation

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ES cells were cultured in feeder-free conditions using N2B27 + 2i media supplemented with 2000U mL−1 of ESGRO (Millipore) on a gelatinized plate. KH2 ES cells72 (link) with epitope-tagged Hoxb1 (3XFLAG-MYC) were used for Hoxb1 ChIP using anti-flag antibody (F1804-Sigma). Unmodified KH2 cell lines were used for ChIP experiments for Pbx (SC-888; Santa Cruz), Meis (SC-25412; Santa Cruz), Prep1 (ab55603; Abcam), Prep2 (sc-292315X; Santa Cruz) and EP300 (Sc-585X; Santa Cruz). Cells were differentiated to neuroectoderm in differentiation media containing DMEM + 10% (vol/vol) Serum + NEAA + 3 µM RA for a requisite length of time. Cells were harvested at 80–90% confluency.
Parker H.J., De Kumar B., Green S.A., Prummel K.D., Hess C., Kaufman C.K., Mosimann C., Wiedemann L.M., Bronner M.E, & Krumlauf R. (2019). A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates. Nature Communications, 10, 1189.
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2

Western Blot Analysis of Prep1

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Cell lysates in NP40 buffer were separated by SDS-PAGE and transferred to PVDF membranes. The blots were then probed with an antibody raised against the N-terminal portion of Prep1 (ab55603, Abcam, Cambridge, MA, USA) and anti-GAPDH antibodies (Santa Cruz, CA), followed by HRP-conjugated secondary antibodies and then visualized by enhanced chemiluminescence (ECL plus). Blots were scanned and analyzed using a Luminescent image analyzer (LAS-3000, FUJIFILM, Japan).
Yoshioka K., Oda A., Notsu C., Ohtsuka T., Kawai Y., Suzuki S., Nakamura T., Mabuchi Y., Matsuzaki Y, & Goitsuka R. (2015). Loss of the Homeodomain Transcription Factor Prep1 Perturbs Adult Hematopoiesis in the Bone Marrow. PLoS ONE, 10(8), e0136107.
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