For Western blotting, AKT (Cell Signaling Technology, 4691, 1:2000), pAKT S437 (Cell Signaling Technology 4060, 1:2000), S6 (Cell Signaling Technology, 2217S, 1:5000), pS6 S235/236 (Cell Signaling Technology 2211S, 1:3000), pS6 S240/244 (Cell Signaling Technology 5364, 1:3000), and PARP (Cell Signaling Technology 9542, 1:1000); for IF, pS6 S235/236 (Cell Signaling Technology 2211S, 1:200), Reelin (Sigma-Aldrich, mab5364, 1:200), CUX1 (Santa Cruz, sc-13024, 1:250, AR), CTIP2 (Abcam, ab18465, 1:500, AR), TBR1 (Abcam, ab31940, 1:500, AR), NeuN (abcam, ab177487,1:300, AR), and GFAP (Dako, Z033429-2, 1:500); finally, for IHC (paraffin sections), somatostatin (Abcam, ab111912, 1:50, AR), parvalbumin (Millepore, MAB1572, 1:200, AR), calretinin (swant, 7697, 1:200, AR), and calbindin (swant, CB38, 1:10000, AR) antibodies were used.
Ab177487
Manufactured by Agilent Technologies
Ab177487 is a laboratory instrument designed for the detection and analysis of specific biomolecules. The core function of this product is to provide accurate and reliable measurements of target analytes in a variety of sample types.
Automatically generated - may contain errors
Lab products found in correlation
Mab5364, by Santa Cruz Biotechnology (1 mentions)
Ab31940, by Abcam (1 mentions)
Ab18465, by Abcam (1 mentions)
Sc 13024, by Abcam (1 mentions)
Ps6 s240 244, by Cell Signaling Technology (1 mentions)
Ps6 s235 236, by Cell Signaling Technology (1 mentions)
Z033429 2, by Agilent Technologies (1 mentions)
Ab11306, by Abcam (1 mentions)
A21432, by Thermo Fisher Scientific (1 mentions)
Z0334, by Merck Group (1 mentions)
A21206, by Thermo Fisher Scientific (1 mentions)
Ab9610, by Thermo Fisher Scientific (1 mentions)
Af2018, by Abcam (1 mentions)
A31571, by Thermo Fisher Scientific (1 mentions)
2 protocols using ab177487
1
Comprehensive Neurological Protein Analysis
2
Immunofluorescence analysis of neural markers
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The following primary antibodies were used for immunofluorescence staining: rabbit anti-Ki67 (Abcam, ab15580, 1:1000), rabbit anti-CIC (Lu et al. 2017; 1:500), mouse anti-DCX (Santa Cruz Biotech, sc-217190; 1:25), goat anti-SOX2 (R&D Systems, AF2018; 1:500), mouse anti-NES (Abcam, ab11306; 1:200), rabbit anti-TBR2 (Abcam, 183,991; 1:500), rabbit anti CALB1 (Swant, CB38; 1:500), rabbit anti-NeuN (Abcam, ab177487; 1:500), rabbit anti-GFAP (DAKO, Z0334; 1:2000), rabbit anti-OLIG2 (Millipore, AB9610; 1:1000), rabbit anti-NFIB (Invitrogen, PIPA552032; 1:200), and rabbit anti-NFIA (Sigma, HPA006111-100UL; 1:200). The secondary antibodies used were donkey anti-rabbit Alexa Fluor 488 (Invitrogen, A21206; 1:1000), donkey anti-mouse Alexa Fluor 647 (Invitrogen, A31571; 1:1000), and donkey anti-goat Alexa Fluor 555 (Invitrogen, A21432; 1:1000).
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