Ebastine, hydroxyebastine, carboxyebastine, desalkylebastine, hydroxyebastine-d5, carebastine-d5, desalkylebastine-d5, bufuralol, hydroxybufuralol, hydroxybufuralol-d9, repaglinide, 2-despiperidyl-2-amino repaglinide (M1), 3′-hydroxyrepaglinide (M4), bupropion, hydroxubupropion, hydroxybupropion-d5, hydroxytolbutamide, carboxytolbutamide, 1′-hydroxytolbutaminde-d9, 4′-carboxytolbutamide-d9, 4-hydroxyMidazolam, 4-hydroxyMidazolam-d5, 6-hydroxyChlorzoxazone and 12-hydroxydodecanoic-d20 acid were purchased from Toronto Research Chemicals (Toronto, ON, Canada). HydroxyChlorzoxazone-d2 was obtained from TLC PharmaChem (Mississauga, ON, Canada). Midazolam and 1′-hydroxyMidazolam-d4 were purchased from Cerilliant (Round Road, TX, USA). Chlorzoxazone, tolbutamide, dodecanoic acid, β-Nicotinamide-Adenine Dinucleotide Phosphate (NADP), glucose-6-phosphate (G6P), glucose-6-phosphate dehydrogenase (G6PD), dimethyl sulfoxide (DMSO), trishydroxymethylaminomethame (TRIS), and phenylmethanesulfonyl (PMSF) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethynediaminetetraacetic acid (EDTA) and dithiothreitol were obtained from Bishop (Burlington, ON, Canada) and from Gibco®, Life Technologies Ltd. (Eugene, OR, USA), respectively. All other chemicals used were commercially available and were of analytical grade.
Phenylmethanesulfonyl pmsf
Manufactured by Merck Group
Sourced in United States, Spain
Phenylmethanesulfonyl (PMSF) is a serine protease inhibitor commonly used in biochemical and molecular biology applications. It functions by irreversibly inhibiting serine proteases, which are enzymes that catalyze the hydrolysis of peptide bonds. PMSF is often utilized in the preparation of protein samples to prevent proteolytic degradation during extraction and purification.
Automatically generated - may contain errors
Lab products found in correlation
Chlorzoxazone, by Merck Group (1 mentions)
Tolbutamide, by Merck Group (1 mentions)
Hydroxytolbutamide, by LGC (1 mentions)
Dodecanoic acid, by Merck Group (1 mentions)
Bupropion, by LGC (1 mentions)
6 hydroxychlorzoxazone, by LGC (1 mentions)
1 hydroxymidazolam d4, by Cerilliant (1 mentions)
Midazolam, by Cerilliant (1 mentions)
Hydroxylamine, by Merck Group (1 mentions)
Bis tris propane, by Merck Group (1 mentions)
9 cis retinal, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
1 2 dimyristoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
2 protocols using phenylmethanesulfonyl pmsf
1
Analytical reference standards for pharmacokinetics
2
Purification and Characterization of Rhodopsin Variants
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Rho was purified from bovine retinas, which were obtained from J.A. Lawson (Lincoln, NE). WT, G90V and N55K opsin genes were cloned into the pMT4 vector. The chromophore 11-cis-retinal was provided by Dr. R. Crouch (National Eye Institute, National Institutes of Health, USA). 9-cis-retinal was purchased from Sigma-Aldrich.
1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMPC(14:0) and 1,2-dihexanoyl-snglycero-3-phosphocholine, DHPC(6:0) were purchased from Avanti Polar Lipids Inc (Alabaster, AL, USA) and n-dodecyl-D-maltoside (DM) was from Anatrace (Maumee, OH, USA).
Purified mAb rho-1D4 was provided by Cell Essentials (Boston, MA, USA) and was coupled to CNBr-activated Sepharose 4B Fast Flow following the manufacturer's instructions (Amersham Biosciences, USA). Secondary antibody goat anti-mouse IgG-HRP conjugated was purchased from Santa Cruz Biotechnology (Heidelberg, Germany).
The 1D4 9-mer peptide corresponding to the last 9 amino acids of Rho (TETSQVAPA) was synthesized by Serveis Cientificotècnics (Universitat de Barcelona, Spain).
Hydroxylamine, protease inhibitor cocktail, phenylmethanesulfonyl (PMSF), and bistris-propane (BTP) were from Sigma-Aldrich (St. Louis, MO, USA). Polyethyleneimine 25 kDa (PEI) was purchased from Polysciences (Warrington, PA, USA).
1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMPC(14:0) and 1,2-dihexanoyl-snglycero-3-phosphocholine, DHPC(6:0) were purchased from Avanti Polar Lipids Inc (Alabaster, AL, USA) and n-dodecyl-D-maltoside (DM) was from Anatrace (Maumee, OH, USA).
Purified mAb rho-1D4 was provided by Cell Essentials (Boston, MA, USA) and was coupled to CNBr-activated Sepharose 4B Fast Flow following the manufacturer's instructions (Amersham Biosciences, USA). Secondary antibody goat anti-mouse IgG-HRP conjugated was purchased from Santa Cruz Biotechnology (Heidelberg, Germany).
The 1D4 9-mer peptide corresponding to the last 9 amino acids of Rho (TETSQVAPA) was synthesized by Serveis Cientificotècnics (Universitat de Barcelona, Spain).
Hydroxylamine, protease inhibitor cocktail, phenylmethanesulfonyl (PMSF), and bistris-propane (BTP) were from Sigma-Aldrich (St. Louis, MO, USA). Polyethyleneimine 25 kDa (PEI) was purchased from Polysciences (Warrington, PA, USA).
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