Phenylmethanesulfonyl pmsf
Phenylmethanesulfonyl (PMSF) is a serine protease inhibitor commonly used in biochemical and molecular biology applications. It functions by irreversibly inhibiting serine proteases, which are enzymes that catalyze the hydrolysis of peptide bonds. PMSF is often utilized in the preparation of protein samples to prevent proteolytic degradation during extraction and purification.
Lab products found in correlation
2 protocols using phenylmethanesulfonyl pmsf
Analytical reference standards for pharmacokinetics
Purification and Characterization of Rhodopsin Variants
1,2-dimyristoyl-sn-glycero-3-phosphocholine, DMPC(14:0) and 1,2-dihexanoyl-snglycero-3-phosphocholine, DHPC(6:0) were purchased from Avanti Polar Lipids Inc (Alabaster, AL, USA) and n-dodecyl-D-maltoside (DM) was from Anatrace (Maumee, OH, USA).
Purified mAb rho-1D4 was provided by Cell Essentials (Boston, MA, USA) and was coupled to CNBr-activated Sepharose 4B Fast Flow following the manufacturer's instructions (Amersham Biosciences, USA). Secondary antibody goat anti-mouse IgG-HRP conjugated was purchased from Santa Cruz Biotechnology (Heidelberg, Germany).
The 1D4 9-mer peptide corresponding to the last 9 amino acids of Rho (TETSQVAPA) was synthesized by Serveis Cientificotècnics (Universitat de Barcelona, Spain).
Hydroxylamine, protease inhibitor cocktail, phenylmethanesulfonyl (PMSF), and bistris-propane (BTP) were from Sigma-Aldrich (St. Louis, MO, USA). Polyethyleneimine 25 kDa (PEI) was purchased from Polysciences (Warrington, PA, USA).
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