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Tween 80

Manufactured by J&K Scientific
Sourced in China

Tween-80 is a non-ionic surfactant commonly used in laboratory applications. It is a polyoxyethylene sorbitan monooleate with the chemical formula C₆₄H₁₂₄O₂₆. Tween-80 functions as a wetting agent, emulsifier, and detergent, and is widely used in various scientific and industrial processes.

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4 protocols using tween 80

1

Synthesis and Purification of PLGA Copolymer

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1-(2-aminoethyl)piperazine (AEPZ, Aldrich), N,N'-bis(acryloyl)cystamine (BAC, Fluka), poly(ethylene glycol) methyl ether (Mn: 750, Aldrich), span80 (J&K) and tween80 (J&K) were purchased and used without further purification. α-Amino-ω-methoxy-poly(ethylene glycol) was prepared from poly(ethylene glycol) methyl ether according to literature [17] . L-lactide and glycolide were purchased from PURAC (the Netherlands) and purified by recrystallization in ethyl acetate (Beijing Tonghua fine chemicals company) twice. Poly(lactic-co-glycolic acid) (PLGA) was prepared by ring-opening polymerization of L-lactide and glycolide under 65 Pa in sealed glass ampoules at 180 °C for 20 h in the presence of stannous octoate as catalyst (0.05 wt%). The raw PLGA (50/50) (85,000) (Molecular weights were determined by GPCmax VE-2001 gel-permeation chromatography) was purified by dissolving in chloroform and re-precipitation from ethanol, followed by drying in vacuum at room temperature for 48 h. The 1,4-dioxane and other reagents were obtained from Beijing Chemical Reagents Company, China and directly used without further treatment. The 1,4-dioxane used in here was not chromatographically pure. All other chemicals were purchased from Sigma-Aldrich.
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2

Synthesis and Characterization of ZL-004

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ZL-004 was synthesized by Guoping Wang team from National Pharmaceutical Engineering and Research Center (Shanghai, China). Tween-80 was purchased from J&K Scientific.Ltd (Beijing, China). PEG 4000 and CMC-Na were purchased from Shanghai Chineway Pharmaceutical Tech. Co. Ltd. (Shanghai, China). Tetrahydrofuran (THF) was purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).
Male Sprague-Dawley (SD) rats (280–300 g) were purchased from the Shanghai Super-B&K Laboratory Animal Corporation Ltd. (Shanghai, China). All the studies were performed in accordance with the Ethical Guidelines for Investigations in Laboratory Animals and were approved by the National Pharmaceutical Engineering and Research Center (2017-0325-001).
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3

Synthesis of BaSO4 Microspheres

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BaSO4 microspheres (1.3~1.8 µm) were prepared in our own laboratory [14 (link)]. CA weighted 30,000 g∙mol−1 of average molecular was procured from Sino Pharm Group (Beijing, China). Tween-80 was procured from J&K Scientific (Beijing, China). Acetone and dehydrated ethanol procured from the Beijing reagent factory (Beijing, China) were employed as solvents. Distilled water was used as a non-solvent. Serum was employed by Ortho-clinical Diagnostics, Inc. (Rochester, NY, USA)
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4

Propiconazole Exposure Protocol

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95% propiconazole was purchased from Yunxin (Beijing) Agricultural Technology Co., Ltd. Stock solution of propiconazole exposure was prepared in dimethyl sulfoxide. The 25% propiconazole emulsifiable concentrate (EC) and propiconazole stock standard solutions of 100 mg/L in acetone were supplied by the College of Science, China Agricultural University. dimethyl sulfoxide (purity 99.7%) and Tween 80 were purchased from J&K Scientific Ltd.
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