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Irdye 800cw 2 dg

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The IRDye 800CW 2-DG is a near-infrared fluorescent dye that can be used to label glucose-derived compounds. It is designed for in vitro and in vivo imaging applications.

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Lab products found in correlation

Platelet derived growth factor bb pdgf bb, by R&D Systems (1 mentions) Tumor necrosis factor (tnf), by R&D Systems (1 mentions) Xf plasma membrane permeabilizer, by Agilent Technologies (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Intracellular ph calibration buffer kit, by Thermo Fisher Scientific (1 mentions) Ff 01 800 12 25, by IDEX Corporation (1 mentions) Zoletil 50, by Virbac (1 mentions) Ketaset, by Zoetis (1 mentions)

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IRDye 800CW (808 citations) IRDye 800CW 2-DG Optical Probe (6 citations)

5 protocols using irdye 800cw 2 dg

1

Metabolic Profiling of Cell Lines

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Lipopolysaccharide (LPS), 2-deoxy-D-glucose (2-DG), 3-bromopyruvate (BrPa), and all reagents for respirometry were obtained from Sigma-Aldrich, except for the XF plasma membrane permeabilizer (used in permeabilized cell experiments), which was obtained from Seahorse Bioscience. Platelet-derived growth factor BB (PDGF-BB) and tumor necrosis factor (TNF) were obtained from R&D Systems. IRDye 800CW 2-DG and IRDye 800CW carboxyl were purchased from Li-Cor Biosciences. MK-2206 (an Akt inhibitor) was obtained from Selleckchem.
Garcia-Carbonell R., Divakaruni A.S., Lodi A., Vicente-Suarez I., Saha A., Cheroutre H., Boss G.R., Tiziani S., Murphy A.N, & Guma M. (2016). Critical Role of Glucose Metabolism in Rheumatoid Arthritis Fibroblast-like Synoviocytes. Arthritis & rheumatology (Hoboken, N.J.), 68(7), 1614-1626.
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2

Fluorescent Dye Buffering Optimization

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A well-plate sample of IRDye 800CW-2DG (LI-COR Biotechnology, NA, USA) was prepared at a constant concentration of 15 μM using seven different buffers: Intracellular pH Calibration Buffer Kit (pH 4.5, pH 5.5, pH 6.5, pH 7.5–ThermoFisher, USA) distilled H2O, PBS (pH 7), and DMSO. Laser excitation was set to 760 nm, and the emission filter used was 800 ± 10 nm (Semrock, FF01-800/12-25).
Smith J.T., Rudkouskaya A., Gao S., Gupta J.M., Ulku A., Bruschini C., Charbon E., Weiss S., Barroso M., Intes X, & Michalet X. (2022). In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera. Optica, 9(5), 532-544.
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3

Real-Time Tumor Localization for Surgical Guidance

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For pre- and intraoperative tumor localization in real-time resection, we conducted in vivo tumor localization assay using IRDye® 800CW 2-DG (2-deoxy-D-glucose) optical probe which was purchased from LI-COR, Biosciences, USA. To evaluate and establish metastatic potential of A549 vs LCN2-A549 TICSCs, characterized cells were inoculated subcutaneously to nude mice. After 7 days experimental metastasis was observed followed by observation of distinct ‘spontaneous metastasis’, where the tumor cells were first allowed to form a primary tumor at the site of injection and then to escape into lymphatic or blood circulation. Probe was dissolved in phosphate buffer saline (1X PBS) and was injected into the tail vein of the tumor-bearing nude mice then mice were observed after they were anesthetized with Zoletil 50 (Virbac, Carros, France) 1 ml/kg intraperitoneally and all surgical procedures were performed under general anesthesia at different time intervals. Metastasis was detected using optical molecular imaging, in particular near-infrared fluorescence (NIRF) range.
MONGRE R.K., SODHI S.S., SHARMA N., GHOSH M., KIM J.H., KIM N., PARK Y.H., SHIN Y.G., KIM S.J., JIAO Z.J., HUYNH D.L, & JEONG D.K. (2015). Epigenetic induction of epithelial to mesenchymal transition by LCN2 mediates metastasis and tumorigenesis, which is abrogated by NF-κB inhibitor BRM270 in a xenograft model of lung adenocarcinoma. International Journal of Oncology, 48(1), 84-98.
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4

Targeted Imaging of Pancreatic Cancer

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IRDye 800CW 2-DG (NIR 2-DG, Li-Cor Biosciences) was dissolved in 1 ml sterile saline at a final concentration of 100 nM. Glyceryl trilinoleate (GTL; TCI America) for in vitro studies was dissolved and sonicated in HEPES containing buffer; for in vivo studies was directly injected into the pancreatic duct of the rats. Ketamine Hydrochloride (Ketaset, Fort Dodge Animal health), Xylazine (Anased, Lloyd Laboratories) and Isoflurane USP (Piramal Healthcare).
de Oliveira C., Patel K., Mishra V., Trivedi R.N., Noel P., Singh A., Yaron J.R, & Singh V.P. (2016). Characterization and Predictive Value of Near Infrared 2-Deoxyglucose Optical Imaging in Severe Acute Pancreatitis. PLoS ONE, 11(2), e0149073.
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5

Fluorescent Labeling of CTM and TZM

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IRDye 800CW 2-DG was purchased at Li-Cor (Lincoln, Nebraska, USA). CTM was purchased at MedChemExpress (Monmouth Junction, NJ, USA), TZM was obtained through Albany Medical Center pharmacy. Fluorescent labeling of TZM is described in depth elsewhere [43 ]. Fluorescent labeling of CTM was performed following protocol described elsewhere [54 ]. Mouse IgG AF700 and rabbit IgG AF750 were purchased at Thermo Fisher Scientific (Waltham, MA, USA).
Smith J.T., Rudkouskaya A., Gao S., Gupta J.M., Ulku A., Bruschini C., Charbon E., Weiss S., Barroso M., Intes X, & Michalet X. (2022). In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera. Optica, 9(5), 532-544.
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