Luna 5 μm pfp 2 4.6 250 mm hplc column

Example 15

Oenothein B (>95% purity) was purified from pomegranate juice by Sephadex LH20 chromatography and reverse-phase HPLC (step 1—Apex Prepsil 8 μm C-18 10×250 mm HPLC column—Jones Chromatography; step 2 Luna 5 μm PFP(2) 4.6×250 mm HPLC column—Phenomenex). Ability to modify endothelial function was assessed by incubating oenothein B with bovine aortic endothelial cells and measuring endothelin-1 synthesis following previously described methods (Journal of Agricultural and Food Chemistry, 2010, 58: 4008-13). As shown by FIG. 1(A), inhibition of endothelin-1 synthesis by oenothein B was found to be concentration dependent.

Example 15

Oenothein B (>95% purity) was purified from pomegranate juice by Sephadex LH20 chromatography and reverse-phase HPLC (step 1—Apex Prepsil 8 μm C-18 10×250 mm HPLC column—Jones Chromatography; step 2 Luna 5 μm PFP(2) 4.6×250 mm HPLC column—Phenomenex). Ability to modify endothelial function was assessed by incubating oenothein B with bovine aortic endothelial cells and measuring endothelin-1 synthesis following previously described methods (Journal of Agricultural and Food Chemistry, 2010, 58: 4008-13). As shown by FIG. 1(A), inhibition of endothelin-1 synthesis by oenothein B was found to be concentration dependent.