Gapdh pph00150f

Manufactured by Qiagen

GAPDH (PPH00150F) is a qPCR primer pair designed for the detection and quantification of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. GAPDH is a widely used reference gene for gene expression analysis. The primer pair is validated for use with real-time PCR and can be used with a variety of species.

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Lab products found in correlation

Direct zol rna miniprep plus kit, by Zymo Research (1 mentions) Lightcycler 480 sybr green, by Roche (1 mentions) Cfx connect real time system, by Bio-Rad (1 mentions) Rnaqueous 4pcr total rna isolation kit, by Thermo Fisher Scientific (1 mentions) Quantitech reverse transcription kit, by Qiagen (1 mentions) Sybr green qpcr mastermix, by Qiagen (1 mentions)

Spelling variants of this product

GAPDH (170 citations) PPH00150F (3 citations)

2 protocols using gapdh pph00150f

EBV B Cell RNA Quantification

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Total RNA was isolated from EBV B cells carrying the rs10499197-tagged TNFAIP3 non-risk or risk haplotype using Direct-zol RNA MiniPrep Plus kit (Zymo Research) according to the manufacturer’s protocol. cDNA synthesis was performed using QuantiTect reverse transcriptase kit (Qiagen) as per the manufacturer’s recommendations. Gene expression was measured by qRT-PCR analysis using Light Cycler480 SYBR Green (Roche Diagnostics). Gene expression primers for human IL20RA (QT00069272), IFNGR1 (QT00089404) and GAPDH (PPH00150F) were purchased from Qiagen.

Pasula S., Gopalakrishnan J., Fu Y., Tessneer K.L., Wiley M.M., Pelikan R.C., Kelly J.A, & Gaffney P.M. (2022). Systemic lupus erythematosus variants modulate the function of an enhancer upstream of TNFAIP3. Frontiers in Genetics, 13, 1011965.

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Quantitative Real-Time PCR Analysis in DEB-Exposed Cells

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Total RNA was isolated from control and DEB-exposed TK6 and/or NH32 cells using the RNAqueous-4PCR Total RNA Isolation kit (Life Technologies, Inc). The isolated RNA (1 μg) was subsequently reverse transcribed using the QuantiTech Reverse Transcription Kit (Qiagen, Inc.). Quantitative real-time PCR was then performed utilizing a SYBR Green qPCR Master Mix (Qiagen, Inc.) and a CFX Connect real-time system (Bio-Rad). The Human CCL4 (PPH00563B-200) and GAPDH (PPH00150F) PCR primers were purchased from Qiagen, Inc. The nanoluciferase PCR primers (Forward primer: 5’AAGGATTGTGCCTGAG-3’; Reverse primer: 5’-AACACGGCGATGCCTTCA-TA-3’) were obtained from Integrated DNA Technologies, Inc. Data were normalized to endogenous glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA levels in each sample. Relative normalized mRNA levels between samples were determined using real time machine software. All reactions were conducted in triplicates, and experiments were repeated three times.

Ewunkem A.J., Deve M., Harrison S.H, & Muganda P.M. (2023). Diepoxybutane induces the p53-dependent transactivation of the CCL4 gene that mediates apoptosis in exposed human lymphoblasts. Journal of biochemical and molecular toxicology, 37(5), e23316.

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