Image lab

Cell and virus lysates in lysis buffer with 6x SDS-PAGE sample loading buffer (600 mM Tris-HCL pH6.8, 30% glycerol, 12% SDS, 20mM DTT, 0.03% bromophenol blue) were heated at 95°C for 5 min. Samples were analyzed on 10% 1.5mm Tris-glycine gels using a BioRad Trans-Blot Turbo Transfer system according to manufacturer’s instructions. Proteins were detected with primary (see Table S2) and secondary antibodies. Protein bands were visualized using chemiluminescence with either a Gel Doc XR+ system (BioRad) or Sapphire Biomolecular Imager (Azure Biosystems) and analyzed with either Image Lab version 6.0.1 or AzureSpot (Azure Biosystems).

Cell and virus lysates were treated with 6x sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample loading buffer [600 mM Tris-HCl (pH 6.8), 30% glycerol, 12% SDS, 20 mM dithiothreitol, 0.03% bromophenol blue] and heated at 95 °C for 5 min. Samples were analyzed on 4 to 15% Tris-glycine gels using a Bio-Rad Trans-Blot Turbo Transfer system according to the manufacturer’s instructions. Proteins were detected with primary and secondary antibodies. Protein bands were visualized using chemiluminescence with Gel Doc XR+ system (Bio-Rad) and analyzed with Image Lab version 6.0.1 or AzureSpot (Azure Biosystems).