Biotin rat anti mouse igg1

Manufactured by BD

Sourced in United States

Biotin-rat-anti-mouse IgG1 is a secondary antibody reagent that binds to the IgG1 subclass of mouse immunoglobulins. It is labeled with biotin, which can be used for detection or purification purposes in various immunoassays and research applications.

Automatically generated - may contain errors

Lab products found in correlation

Biotin rat anti mouse ige, by BD (2 mentions) Streptavidin hrp, by R&D Systems (2 mentions) Anti mouse cd16 cd32, by BD (1 mentions) Lps from e coli, by Merck Group (1 mentions) Facscalibur, by FlowJo (1 mentions) Fitc rat anti mouse cd45r b220, by BD (1 mentions) Interleukin 4 (il 4), by Merck Group (1 mentions) Anti cd43 macs micro beads, by Miltenyi Biotec (1 mentions) 96 well plate, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg fc hrp conjugated antibody, by Merck Group (1 mentions) Biotin rat anti mouse igg2a, by BD (1 mentions) Emax microplate reader, by Molecular Devices (1 mentions) Tmb substrate, by BD (1 mentions) Biotin rat anti mouse igg2b, by BD (1 mentions) Casein, by Thermo Fisher Scientific (1 mentions) Streptavidin hrp, by Jackson ImmunoResearch (1 mentions) Separate 96 well plates, by Greiner (1 mentions) Sodium phosphate dibasic, by Mallinckrodt (1 mentions) 1 n hcl, by Thermo Fisher Scientific (1 mentions) Peroxidase conjugated goat antimouse igm, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Mouse IgG1 (48 citations) Rat anti-mouse IgG1 (9 citations) Rat IgG1 (6 citations) Anti-IgG1 biotin (5 citations) IgG1-biotin (2 citations) Mouse anti (2 citations)

5 protocols using biotin rat anti mouse igg1

CFSE-Labeled Mouse B Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

B cells were isolated by lysing red blood cells with ACK lysing buffer, followed by reverse selection using anti-CD43 MACS micro-beads (Miltenyi Biotech 130-049-801) and a Mini-MACS column. Collected B cells were stained with 5 μM CFSE for 10 minutes at 37°C and then cultured in RPMI-1640 containing 10% FBS, 1x penicillin/streptomycin, 1% L-glutamine, 1x MEM non-essential amino acids, 1% sodium pyruvate, 1% HEPES, and 53 mM 2-mercaptoethanol. Media was supplemented with 25 μg/ml LPS from E.coli (Sigma L2630), 50 U/ml Interleukin-4 (Sigma I1020), and 1:1000 rat anti-mouse anti-CD180 (BD Pharmingen 552128). Cells were cultured for 72 hours with VX-984 (0.2, 0.4, and 0.8 μM) at 37°C with 5% CO₂.
B cells were blocked with 5 μl of anti-mouse CD16/CD32 (BD pharmingen 553141). Primary antibodies Biotin-rat anti-mouse IgG1 (BD Pharmingen 550331) and FITC-rat anti-mouse CD45R/B220 (BD Pharmingen 553088) were added at 10 μl of a 1:100 dilution, followed by 10 μl of a 1:100 dilution of Streptavidin-A647 (Life Technologies S32357). Cell proliferation and IgG+ cells were measured using a Becton Dickinson FACSCalibur and data were analyzed by FlowJo software.

Khan A.J., Misenko S.M., Thandoni A., Schiff D., Jhawar S.R., Bunting S.F, & Haffty B.G. (2018). VX-984 is a selective inhibitor of non-homologous end joining, with possible preferential activity in transformed cells. Oncotarget, 9(40), 25833-25841.

+ Open protocol

+ Expand

SARS-CoV-2 Spike Protein Antibody Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

96-well plate (Thermo Fisher Scientific, MA, USA) were coated with 5 µg/ml of WT, Beta, Delta, or Omicron RBD or spike protein at 4 °C overnight. Plates were then blocked with 3% skim-milk/PBS at room temperature for 2 h. Serum samples were serially diluted and added to the blocked plates before incubation at room temperature for an hour. Following incubation, bound antibodies were either detected with goat anti-mouse IgG Fc HRP-conjugated antibody (Chemicon, CA, USA) for total IgG assessment or biotin-rat-anti-mouse IgG1 (BD Biosciences, NJ, USA) and biotin-rat-anti-mouse IgG2a (BD Biosciences, NJ, USA), and then followed by HRP- streptavidin (R&D Systems, MN, USA) for IgG subclass assessment. Plates were developed by TMB substrate (BD Biosciences, NJ, USA) and the reactions were stopped by adding 2N H₂SO₄. The absorbance at 450 nm were measured with EMax Microplate reader (Molecular Devices, CA, USA). The endpoint dilution titer was determined when titer value of the last serum dilution was twofold above the blank value.

Lee I.J., Sun C.P., Wu P.Y., Lan Y.H., Wang I.H., Liu W.C., Yuan J.P., Chang Y.W., Tseng S.C., Tsung S.I., Chou Y.C., Kumari M., Lin Y.S., Chen H.F., Chen T.Y., Lin C.C., Chiu C.W., Hsieh C.H., Chuang C.Y., Cheng C.M., Lin H.T., Chen W.Y., Hsu F.F., Hong M.H., Liao C.C., Chang C.S., Liang J.J., Ma H.H., Chiang M.T., Liao H.N., Ko H.Y., Chen L.Y., Ko Y.A., Yu P.Y., Yang T.J., Chiang P.C., Hsu S.T., Lin Y.L., Lee C.C., Wu H.C, & Tao M.H. (2022). A booster dose of Delta × Omicron hybrid mRNA vaccine produced broadly neutralizing antibody against Omicron and other SARS-CoV-2 variants. Journal of Biomedical Science, 29, 49.

+ Open protocol

+ Expand

Quantification of Antigen-Specific Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole blood was collected on day 21. Clotting was allowed overnight at 4°C and serum was recovered after centrifugation for 10 min at 8000 xg. Samples were serially diluted 1:1 in assay diluent and incubated on antigen-medium-binding pre-coated plates (10 μg/mL OVA or 50 μg/mL ClfA in sodium carbonate buffer, pH 9.5). Ag-specific serum Ab titers were measured using a standard ELISA protocol and the following antibodies: goat anti-mouse total IgG-HRP from Southern Biotech (1/5000 dilution), biotin rat anti-mouse IgG1 from BD (1/4000 dilution), biotin rat anti-mouse IgG2b from BD (1/4000 dilution), and goat anti-mouse IgG2c-HRP from AbD Serotec (1/2000 dilution) and developed using streptavidin-HRP or OPD substrate directly as required. OD values were obtained using a VersaMax microplate reader at 492nm. Antibody concentrations were expressed as Log10 endpoint titers calculated by regression of a curve of OD values versus reciprocal serum levels to a cutoff point of 4 SD above the blank.

Muñoz-Wolf N., Ward R.W., Hearnden C.H., Sharp F.A., Geoghegan J., O’Grady K., McEntee C.P., Shanahan K.A., Guy C., Bowie A.G., Campbell M., Roces C.B., Anderluzzi G., Webb C., Perrie Y., Creagh E, & Lavelle E.C. (2023). Non-canonical inflammasome activation mediates the adjuvanticity of nanoparticles. Cell Reports Medicine, 4(1), 100899.

+ Open protocol

+ Expand

ELISA for Mouse Antibody Isotypes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Based on the antigen used for immunizations, NP conjugated to BSA at a >20 ratio (Biosearch Technologies), PE or OVA all diluted in PBS were coated onto separate 96 well plates (Greiner Bio-one) at 2 μg/mL and incubated overnight at 4 ^°C. Plates were washed then blocked with casein (Thermo Scientific) for one hour at room temperature (RT). Serum samples were serially diluted in casein then added to the plates and incubated for two hours at RT. After washing, biotin rat anti-mouse IgG1 (BD), peroxidase conjugated goat anti-mouse IgM (Jackson Immunoresearch) or biotin rat anti-mouse IgE (BD) was added to the plates and incubated for one hour at RT. Plates were washed and streptavidin HRP (Jackson Immunoresearch) was added to the IgG1 and IgE plates and incubated for thirty minutes at RT. Plates were washed then developed for twenty minutes with an o-Phenylenediamine dihydrochloride tablet (Sigma) dissolved in 100 mM Sodium phosphate dibasic (Mallinckrodt), 50 mM citric acid (Mallinckrodt) and 0.04% hydrogen peroxide (Mallinckrodt) at pH 5.0. Reactions were stopped using 1N HCL (Fisher Scientific) and plates were read at 490λ using a Molecular Devices Spectra Max 340 PC running Soft Max Pro 3.1.2. All antibody data are presented at IgM (1:1000), IgG1 (1:80,000), and IgE (1:100) serum dilutions which represent non-saturating concentrations.

Rose II W.A., Okragly A.J., Patel C.N, & Benschop R.J. (2015). IL-33 released by alum is responsible for early cytokine production and has adjuvant properties. Scientific Reports, 5, 13146.

+ Open protocol

+ Expand

Quantifying HDM-Specific Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total serum IgE was assessed using OptEIA ELISA kits (BD Biosciences, San Diego, CA) according to the manufacturer’s instructions. An indirect ELISA method was used to assess the HDM-specific IgE and IgG1 levels in serum samples. Briefly, 96-well microtiter plates were coated overnight with 100㎍/mL HDM in PBS. The next day, 200㎕ of blocking solution (1% BSA in PBS) was added to the plate before the addition of serum samples that had been diluted 1:200 (for HDM-specific IgG1) in blocking buffer, or undiluted (for HDM-specific IgE) for 1h. Subsequently, 100㎕ of sample was added to the plate for 2 h, followed by biotin-rat-anti-mouse IgE or biotin-rat-anti-mouse IgG1 (2㎍/ml; BD Biosciences, San Diego, CA) for 1 h. To detect biotin-labeled IgE or IgG1, streptavidin-HRP (1:100, R&D Systems, Minneapolis, MN) was added to the plate for 30 min. Next, a tetramethylbenzidine substrate reagent set (1:1, BD Bioseicences) was added to detect levels of IgE or IgG1.

Choi J., Choi B.K., Kim J.S., Lee J.W., Park H.A., Ryu H.W., Lee S.U., Hwang K.W., Yun W.K., Kim H.C., Ahn K.S., Oh S.R, & Lee H.J. (2016). Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma. PLoS ONE, 11(11), e0167098.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!