Cary eclipse fluorescence spectrofluorometer

Manufactured by Agilent Technologies

Sourced in United States

The Cary Eclipse fluorescence spectrofluorometer is a laboratory instrument designed for the analysis of fluorescent samples. It provides accurate and reliable measurements of fluorescence intensity, excitation and emission spectra, and other fluorescence-based parameters.

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Lab products found in correlation

Cary 60 uv vis spectrophotometer, by Agilent Technologies (2 mentions) 710 laser scanning confocal microscope, by Zeiss (1 mentions) Bovine carbonic anhydrase 2, by Merck Group (1 mentions) Alkaline phosphatase, by Merck Group (1 mentions) Avance neo 400 spectrometer, by Bruker (1 mentions) Amplex red, by Thermo Fisher Scientific (1 mentions) Amplex red, by Merck Group (1 mentions) Horseradish peroxidase hrp, by Merck Group (1 mentions) V 670 uv vis nir spectrophotometer, by Jasco (1 mentions) Neosys 2000, by Scinco (1 mentions) Lc ms 2010a, by Shimadzu (1 mentions) Amx 400 spectrometer, by Bruker (1 mentions)

Close spelling variants of this product

Cary Eclipse spectrofluorometer Eclipse Fluorescence Spectrofluorometer Eclipse spectrofluorometer Cary Eclipse Spectrofluorometer

7 protocols using cary eclipse fluorescence spectrofluorometer

Fluorescence Spectra of Lipid Dispersions

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The fluorescence
spectra of dispersions of compound 1, 2,
or 3, containing 0.05 mol % of 6-lauroyl-2-(dimethylamino)naphthalene
(Laurdan) were recorded at four different temperatures (10, 25, 37,
and 50 °C) using a Cary Eclipse fluorescence spectrofluorometer
(Agilent) (λ_ex = 360 nm; λ_em = 400–600
nm). Samples were prepared following the same protocol described above,
but adding 0.05 mol % Laurdan from a 10 mM stock solution in MeOH
for the preparation of the lipid film.

Fracassi A., Podolsky K.A., Pandey S., Xu C., Hutchings J., Seifert S., Baiz C.R., Sinha S.K, & Devaraj N.K. (2023). Characterizing the Self-Assembly Properties of Monoolein Lipid Isosteres. The Journal of Physical Chemistry. B, 127(8), 1771-1779.

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Synthetic Reagents and Biophysical Studies

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All synthetic reagents were purchased from commercial sources and used without further purification unless otherwise noted. All reactions were run under N₂ atmosphere unless otherwise noted. All reverse-phase purification was performed using a Biotage Isolera One Flash Chromatography Instrument. NDM-1, VIM-2, and IMP-1 were expressed and purified as described previously. [20 –22 (link)] Bovine carbonic anhydrase II and alkaline phosphatase were purchased from Sigma Aldrich. Phosphotriesterase was kindly provided by Prof. Frank Raushel from Texas A&M University. For all spectroscopic studies, 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), pH 7.0 containing 10 μM ZnSO₄ was prepared as the buffer. ¹H and ¹³C NMR spectra were recorded on a 400 MHz Agilent MR Spectrometer or a 400 MHz Bruker AVANCE NEO400 Spectrometer, prepared in deuterated acetonitrile, acetone, or methanol. The residual solvent peaks were used as an internal standard. Spectroscopic studies were performed using an Agilent Cary 60 UV–Vis spectrophotometer. Fluorescence spectroscopic measurements were made using an Agilent Cary Eclipse fluorescence spectrofluorometer. Confocal imaging was performed on a Zeiss 710 Laser Scanning Confocal Microscope. Data analysis was performed using GraphPad Prism Version 8.4.3.

Price S., Mehta R., Tan D., Hinojosa A., Thomas P.W., Cummings T., Fast W, & Que E.L. (2022). Structural insights into the design of reversible fluorescent probes for metallo-β-lactamases NDM-1, VIM-2, and IMP-1. Journal of inorganic biochemistry, 233, 111869.

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Turbidity and Rayleigh Light Scattering of BLG Aggregation

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The turbidity and Rayleigh light scattering (RLS) measurements were carried out to detect the SY dye-induced aggregation of BLG at pH 2.0. The turbidity was measured by Cary 60 UV–vis Spectrophotometer (Agilent technologies, Inc. USA, Santa Clara, CA, USA) and Cary Eclipse Fluorescence Spectrofluorometer Agilent technologies, Inc. USA, was used to perform the RLS study. The turbidity of aggregated and non-aggregated samples was measured by taking absorbance at 650 nm. However, the RLS was measured of SY-induced aggregated samples by excitation of samples at 650 nm and the emission of the samples was recorded at 650 nm. BLG (0.2 mg/mL) samples were treated with different SY concentrations (0.0 to 5.0 mM) at pH 2.0. The data of turbidity and RLS was plotted against SY concentrations.
The turbidity was also measured of samples containing BLG (0.2 mg/mL) treated with 0.2 mM SY dye concentrations at different pHs.

Khan J.M., Malik A., Husain F.M., Hakeem M.J, & Alhomida A.S. (2022). Sunset Yellow Dye Induces Amorphous Aggregation in β-Lactoglobulin at Acidic pH: A Multi-Techniques Approach. Polymers, 14(3), 395.

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Amplex Red Assay for Midgut H2O2

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H₂O₂ was measured with Amplex Red (Invitrogen) following the recommendations of the manufacturer with minor modifications. The midgut epithelia of sugar-fed mosquitoes were dissected in 2.5% BSA in PBS, the gut contents were washed out and the epithelia (pools of 5 organs) were incubated in PBS under dim light at room temperature in the presence of Amplex Red (40 μM) and 4 U horseradish peroxidase (HRP, Sigma). After a 30 min incubation, the epithelia were centrifuged, and the supernatants were collected and evaluated for fluorescence emission at 530/590 nm (Ex/Em) in a Varian Cary Eclipse Fluorescence Spectrofluorometer. The resulting values were subtracted from fluorescence readings generated by nonspecific Amplex Red oxidation by the midgut epithelia (pools of 5 organs) in the absence of HRP.

Oliveira J.H., Talyuli O.A., Goncalves R.L., Paiva-Silva G.O., Sorgine M.H., Alvarenga P.H, & Oliveira P.L. (2017). Catalase protects Aedes aegypti from oxidative stress and increases midgut infection prevalence of Dengue but not Zika. PLoS Neglected Tropical Diseases, 11(4), e0005525.

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Upconverted Emission Measurements in Vesicles

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Electronic absorption spectra were acquired on a Jasco V670 UV/vis NIR spectrophotometer using a quartz cuvette with a 1 cm path length. Fluorescence spectra were collected on a Varian Cary Eclipse fluorescence spectrofluorometer. All photophysical measurements were performed at room temperature (293 K).
The TTA-UC measurements were carried out in the same fluorescence spectrophotometer using an additional laser purchased from Edmund Optics with 532 nm excitation of 10 mW power with a 1 mm beam diameter. The TTA-UC measurements were performed in a fluorescence spectrophotometer by blocking the excitation line in the bioluminescence measurement mode. The upconverted emission in vesicles was recorded in the presence of 20 mM sodium sulfite as an oxygen scavenger. The upconverted emission was recorded across the range of 400-500 nm to avoid interference from the excitation source.

Prabhakaran A., Jha K.K., Sia R.C.E., Arellano Reyes R.A., Sarangi N.K., Kogut M., Guthmuller J., Czub J., Dietzek-Ivanšić B, & Keyes T.E. (2024). Triplet-Triplet Annihilation Upconverting Liposomes: Mechanistic Insights into the Role of Membranes in Two-Dimensional TTA-UC. ACS applied materials & interfaces, 16(22).

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Optical Properties of CdSe and CdSxSe1-x QDs

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Optical properties of CdSe and CdS_xSe_1-x quantum dots were checked using an ultraviolet–visible (UV–Vis) spectrophotometer and a fluorescence spectrophotometer. Absorption spectra of quantum dots were collected using a Scinco Neosys-2000 double-beam UV–Vis spectrophotometer. Emission and excitation spectra of nanocrystals were collected using a Varian Cary Eclipse fluorescence spectrofluorometer. Emission spectra of CdSe and CdS_xSe_1-x quantum dots were recorded by using excitation wavelength (λ_exc) 350 nm. Each sample was dissolved in 10 mL of toluene after purification and diluted by 10 factor before characterization in order to prevent errors resulting from self-absorption. The same samples were used to collect the excitation spectrum. Excitation spectra of CdSe quantum dots were collected at two wavelengths; 450 nm and 580 nm. Excitation spectra of CdS_xSe_1-x quantum dots were collected at 525 nm.

GÜLEROĞLU G, & ÜNLÜ C. (2021). Spectroscopic investigation of defect-state emission in CdSe quantum dots. Turkish Journal of Chemistry, 45(3), 520-527.

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Binding Interaction of Compound 1 with HSA

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1 H NMR and 13 C NMR spectra were taken on a Bruker AMX400 spectrometer (Bruker, German). Chemical shifts (δ) were reported in ppm relative to a Me4Si standard in CDCl3. Electrospray ionization (ESI) mass spectra were measured with an LC-MS 2010A (Shimadzu, Japan) instrument.
All fluorescence spectra were carried out on a Cary eclipse fluorescence spectrofluorometer (Varian, USA) with 1.0 cm quartz cell. The fluorescence spectra were recorded in the range of 620 -800 nm with excitation wavelength at 600 nm. The excitation and emission slit is 10 nm. The final concentration of 1 was 10 μM and the concentration of HSA was between 0 and 13.3 μg/mL in a mixture of Tris (10 mM, pH 7.4) and ethanol (95:5, v/v).

Zeng X., Ma M., Zhu B, & Zhu L. (2016). A Near Infrared Fluorescent Probe for Sensitive Determination of Human Serum Albumin. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 32(12).

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