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Andrographolide andro

Manufactured by Merck Group
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Andrographolide (Andro) is a natural compound isolated from the plant Andrographis paniculata. It is a commonly used laboratory reagent for research purposes. Andro serves as a versatile chemical building block for the synthesis of various compounds and derivatives. Its core function is to provide researchers with a source material for further chemical transformations and investigations.

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Lab products found in correlation

Rabbit anti timp3 ab39184, by Abcam (1 mentions) Rabbit anti gapdh 2118, by Cell Signaling Technology (1 mentions) Matrigel, by BD (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Promega (1 mentions) Trypsin, by HiMedia (1 mentions) Oligomycin, by Merck Group (1 mentions) 1324 wn cf, by R&D Systems (1 mentions) Cyt b, by Merck Group (1 mentions) 2 deoxy d glucose 2 dg, by Merck Group (1 mentions)

Close spelling variants of this product

Andro Andrographolide

3 protocols using andrographolide andro

1

Andrographolide Modulation of miR-21

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Andrographolide (Andro, Sigma-Aldrich) was dissolved in dimethyl sulfoxide (DMSO). miRNA mimics of miR-21-5p and negative control RNA (NC) were obtained from RiboBio Co., Ltd. The following primary antibodies were used for immunoblotting: rabbit anti-TIMP3 (ab39184), obtained from Abcam (Cambridge, UK); and rabbit anti-GAPDH (#2118), obtained from Cell Signaling Technology, Inc. (Danvers, Massachusetts, USA).
Dai J., Lin Y., Duan Y., Li Z., Zhou D., Chen W., Wang L, & Zhang Q.Q. (2017). Andrographolide Inhibits Angiogenesis by Inhibiting the Mir-21-5p/TIMP3 Signaling Pathway. International Journal of Biological Sciences, 13(5), 660-668.
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2

Anticancer Effects of Andrographolide

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Andrographolide (Andro) was obtained from Sigma Aldrich (St. Louis, MO, USA, Product number 365645; Lot # MKCF4812) and prepared as a 100 mM stock solution in DMSO (dimethylsulphoxide), further diluted with PBS. MTT (3-(4, 5-dimethylthiazole-2-yl)-2, 5-biphenyl tetrazolium bromide) (Promega, Madison, Wisconsin, USA). Matrigel (BD Biosciences, San Diego, CA, USA), transwell chambers (8 μm, Gyeonggi-do, Korea, genetix Biotech Asia Pvt. Ltd.). DMEM (Dulbecco's Modified Eagle Medium) with high glucose (#AL007S), foetal bovine serum (#RM9955) and trypsin were obtained from Himedia Laboratories (India). Primary antibodies, anti-Bcl-2 (MA5-11757) and anti-Bax (MA5-14003) obtained from Invitrogen. Anti-NF-kB p-65 (mAb#3036) anti-COX-2 (SC-19999), anti-PI3K p-85α (SC-1637), anti-GAPDH (SC-25778), anti-actin (SC-4778) and all the secondary antibodies obtained from Santa Cruz Biotechnology (Santa Cruz, California, USA.). Anti-AKT (#4685), anti-p-AKT (#4060), procured from Cell Signaling Technology (Cell Signaling Technology, Inc., USA). Human cervical cancer cell lines (HeLa and SiHa), human embryonic kidney (HEK-293) cells were obtained from the National Centre for Cell Science (NCCS) Pune, India and were grown in Dulbecco Modified Eagle's Medium (DMEM) supplemented with 10% FBS and 1% penicillin and streptomycin antibiotics. The cells were incubated at 37° C in a 5% CO2 incubator.
Pasha A., Kumar K., Heena S.K., Arnold Emerson I, & Pawar S.C. (2024). Inhibition of NF-kB and COX-2 by andrographolide regulates the progression of cervical cancer by promoting PTEN expression and suppressing PI3K/AKT signalling pathway. Scientific Reports, 14, 12020.
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3

Wnt3a and Glycolysis Modulation in Neurons

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The neurons and slices were treated with recombinant Wnt3a (rWnt3a, 300 ng/mL, 0–24 h, cat: 1324-WN/CF, R&D Systems, USA), Aβ (5 μM), dickkopf-1 (Dkk1, 300 ng/mL, 0–24 h, antagonist of Wnt3a, as internal control, cat: 5897-DK/CF R&D System, USA), cytochalasin B (Cyt B, 1 μM, 3 h, inhibitor of GLUT transporters, this concentration is the IC50 value, in the uptake we used 20 μM, Sigma-Aldrich USA), AZD5356 (20 nM, 12 h, an inhibitor of all Akt isoforms, cat: 5773, TOCRIS, UK), andrographolide (ANDRO, 50 μM, 12 h, agonist of Wnt3a signaling, Sigma-Aldrich USA), carbonate of lithium (Li, 10 mM, 12 h, agonist of Wnt3a signaling, Sigma-Aldrich USA), oligomycin (2 mM, inhibitor of ATP synthase, cat: 861944, Sigma-Aldrich USA) and 2-deoxy-D-glucose (2-DG, 7 mM, as a competitive inhibitor of hexokinase, Sigma-Aldrich USA). Neurons were carefully examined under the microscope to ensure that only plates showing uniform neuronal growth were used. After different treatments, cells were used in different metabolic assays.
Cisternas P., Zolezzi J.M., Martinez M., Torres V.I., Wong G.W, & Inestrosa N.C. (2018). Wnt-induced activation of glucose metabolism mediates the in vivo neuroprotective roles of Wnt signaling in Alzheimer disease. Journal of neurochemistry, 149(1), 54-72.
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