Antibodies specific for tubulin

Manufactured by Merck Group

Sourced in Sao Tome and Principe

Antibodies specific for tubulin are laboratory reagents used to detect and visualize the tubulin protein, a major component of the cytoskeleton in eukaryotic cells. These antibodies can be utilized in various experimental techniques, such as Western blotting, immunofluorescence, and immunohistochemistry, to study the distribution and dynamics of tubulin within cells.

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Lab products found in correlation

Cenp b, by Abcam (2 mentions) Tcs sp5 2 confocal microscope, by Leica (2 mentions) Hoescht 33342, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Specific antibodies (8 citations) Tubulin antibodies (3 citations) Antibodies specific for Beta-Tubulin (2 citations)

2 protocols using antibodies specific for tubulin

Immunofluorescence Imaging of Tubulin and Centromeres

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HeLa cells were incubated in the absence or presence of a test compound for 4 h prior to fixation with 3.7% formaldehyde in phosphate-buffered saline (PBS) and permeabilization in 0.1% Triton X-100 in PBS. Cells were then briefly blocked with 3% Bovine Serum Albumin in PBS, and then probed with antibodies specific for tubulin (Sigma, St. Louis, MO) and the centromere marker CENP-B (Abcam, Cambridge, MA). Hoescht 33342 (Life Technologies) was included to highlight DNA. Samples were imaged using a Leica TCS-SP5 II confocal microscope at the Core University Research Resources Laboratory at New Mexico State University, and figures were prepared using Adobe Photoshop CS5.

Dasari R., Błauż A., Medellin D.C., Kassim R.M., Viera C., Santarosa M., Turner D., van der Westhuyzen A.E., van Otterlo W.A., Olivas T., Yildiz T., Betancourt T., Shuster C.B., Rogelj S., Rychlik B., Hudnall T., Frolova L.V, & Kornienko A. (2019). Microtubule-Targeting 7-Deazahypoxanthines Derived from Marine Alkaloid Rigidins: Exploration of the N3 and N9 Positions and Interaction with Multi-Drug Resistance Proteins. ChemMedChem, 14(3), 322-333.

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Immunofluorescence Analysis of Mitotic HeLa Cells

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HeLa cells were incubated in the absence or presence of 7 for 4 h prior to fixation with 3.7% formaldehyde in phosphate buffered saline (PBS) and permeabilization in 0.1% Triton X-100 in PBS. Cells were then briefly blocked with 3% bovine serum albumin in PBS and then probed with antibodies specific for tubulin (Sigma, St. Louis, MO) and CENP-B (Abcam, Cambridge, MA). Hoescht 33342 (Life Technologies) was included to highlight DNA. Samples were imaged using a Leica TCS-SP5 II confocal microscope at the Core University Research Resources Laboratory at New Mexico State University.

Medellin D.C., Zhou Q., Scott R., Matthew Hill R., Frail S.K., Dasari R., Ontiveros S.J., Pelly S.C., van Otterlo W.A., Betancourt T., Shuster C.B., Hamel E., Bai R., LaBarbera D.V., Rogelj S., Frolova L.V, & Kornienko A. (2015). Novel Microtubule-Targeting 7-Deazahypoxanthines Derived from Marine Alkaloid Rigidins with Potent in Vitro and in Vivo Anticancer Activities. Journal of medicinal chemistry, 59(1), 480-485.

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