We used antibody cocktails for T-lymphoyctes, NK cells. Unless stated otherwise, they were purchased from BioLegend (BioLegend Europe, Uithoorn, the Netherlands):
T-lymphocytes antibody cocktail. PerCP labeled anti-human CD3 (clone UCHT1), APC/Cy7 labeled anti-human CD4 (clone OKT4), PE/Cy7 labeled anti-human CD25 (clone BC96), Alexa Fluor 488 labeled anti-mouse/rat/human FOXP3 (clone 150D), Alexa Fluor 488 labeled mouse IgG1 isotype control (clone MOPC-21), PE labeled anti-mouse/human Tbet (clone eBio4B10, eBioscience, Germany), PE labeled mouse IgG1 isotype control (clone MOPC-21), Alexa Fluor 647 anti-mouse/human GATA-3 (clone TWAJ, eBioscience) and Alexa Fluor 647 mouse IgG1 isotype control (MOPC-21).
Natural Killer cells antibody cocktail. PerCP labeled anti-human CD3 (clone UCHT1), APC labeled anti-human CD56 (clone MEM188), Pacific Blue labeled anti-human CD16 (clone eBioCB16, eBioscience) and PE labeled anti-human CD335 (clone 9E2), PE labeled mouse IgG1 isotype control (clone MOPC-21).
Monocytes antibody cocktail. PerCP/Cy5.5 labeled anti-human CD14 (clone HCD14), Pacific Blue labeled anti-human CD16 (clone eBioCB16, eBioscience), FITC labeled mouse anti-human HLA-DR, DP, DQ (i.e. MHC-II; clone Tu39, BD Pharmingen, Breda, Netherlands), PE labeled anti-human CD62L (clone DREG-56) and PE labeled mouse IgG1 isotype control (clone MOPC-21).
Groen B., van der Wijk A.E., van den Berg P.P., Lefrandt J.D., Gerrit van den B.e.r.g., Sollie K.M., de Vos P., Links T.P, & Faas M.M. (2015). Immunological Adaptations to Pregnancy in Women with Type 1 Diabetes. Scientific Reports, 5, 13618.
+ Open protocol