The following primary antibodies were used for immunohistochemistry experiments with indicated final concentrations: mouse monoclonal anti-ppERK1/2 (M9692, 1:1000/1.5-2 μg/mL, Sigma), rabbit monoclonal anti-β-Catenin (#9562,1:200, CST), chicken monoclonal IgY anti-GFP (A10262, 5 μg/mL, Invitrogen), rat anti-HA (#3K10, 1:400, Roche), mouse anti-F59 Myosin Heavy Chain (AB528373, 1:10/ 0.2-0.5 μg/mL, DSHB), and rabbit anti-pFAK (44-624G, 1:400, Invitrogen). Primary antibodies were targeted with 1:200 dilutions of Alexa Fluor 488 goat anti-chicken IgG H+L (A11039, Invitrogen), Alexa Fluor 594 goat anti-mouse IgG H+L (A11005, Invitrogen), Alexa Fluor 647 goat anti-rabbit IgG (A21245, Invitrogen) or Alexa Fluor 647 goat anti-rat IgG H+L (A21247, Invitrogen) for corresponding primary species. Alexa Fluor 488 Phalloidin (A12379, 1:200, Thermo Fisher) was used against F-actin of muscle fibers.
Alexa fluor 488 goat anti chicken igg h l
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa Fluor 488 goat anti-chicken IgG H+L is a fluorescently-labeled secondary antibody used for the detection and visualization of chicken immunoglobulins (IgG) in various immunoassays and imaging applications. The Alexa Fluor 488 dye provides a bright green fluorescent signal.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 594 conjugate, by Thermo Fisher Scientific (2 mentions)
Mab377, by Merck Group (2 mentions)
Fluoromount g, by Southern Biotech (2 mentions)
Gfp 1020, by Aves Labs (2 mentions)
Alexa fluor 594 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
M9692, by Merck Group (1 mentions)
Alexa fluor 647 goat anti rat igg h l, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Hmga2, by Cell Signaling Technology (1 mentions)
Alexa fluor 555 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Vimentin, by Abcam (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
A11039, by Thermo Fisher Scientific (1 mentions)
4 protocols using alexa fluor 488 goat anti chicken igg h l
1
Immunohistochemistry Antibody Staining Protocol
2
Dental Pulp Stem Cell Characterization
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Deciduous teeth were collected from 3- to 11-year-old patients (n = 3) and permanent teeth were collected from adult patients (19 to 81 years of age, n = 3). Teeth were cracked open and pulps were collected and snap frozen in optimal cutting temperature (OCT) cryomount (Histolab, Gothenburg, Sweden) for subsequent 5 μm sectioning. The sections were fixed for 20 minutes in 4% paraformaldehyde (Merck, Darmstadt, Germany), rinsed and then incubated overnight at room temperature with primary antibodies against HMGA2 (1:500, Cell Signaling Technology) and vimentin (1:200, Abcam). Additional staining was performed the following day with 4',6-diamidino-2-phenylindole (DAPI) (Invitrogen, Burlington, Canada) at 300 nM applied for three minutes. Secondary antibodies used were Alexa Fluor® 555 Donkey Anti-Rabbit IgG (H + L) and Alexa Fluor® 488 Goat Anti-Chicken IgG (H + L) (Invitrogen, Eugene, OR, USA). Control sections were incubated where the primary antibody was omitted. This resulted in no detectable artifact. Additional negative control stainings were performed with isotype control for rabbit primary antibody (Invitrogen, Eugene, OR, USA), which resulted in no unspecific staining. Confocal microscopy was performed with Zeiss LSM700 CLSM and image processing was carried out with Imaris software.
3
Immunostaining of Geniculate Ganglia
4
Immunohistochemical Staining of Geniculate Ganglia
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Geniculate ganglia were dissected, fixed with 4% paraformaldehyde, washed with PBS, cryoprotected overnight with 30% sucrose, embedded in OCT, and cut at 25 μm. Sections were permeabilied with 1% Triton-X in PBS, treated with 4% normal goat serum in PBS followed by Avidin/Biotin Blocking kit, and immunostained with anti-NeuN (1:1,000, clone A60, biotin-conjugated, MAB377, EMD Millipore) and anti-GFP (1:1,000, GFP-1020, Aves Labs, Inc.). Secondary antibodies were Streptavidin, Alexa Fluor 594 conjugate (1:1,000, S-11227, Life Technologies) and Alexa Fluor 488 goat anti-chicken IgG (H+L) (1:1,000, A-11039, Life Technologies). Sections were mounted with Fluoromount-G (0100-01, Southern Biotech).
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