This was performed as described [27] (link), [31] (link) using a 1:100 dilution of rabbit anti-MCM2 or -MCM3 (#4007 and 4102; Cell Signaling Technology, Beverly, MA), anti-Ki67 (ab15580; Abcam, Cambridge, MA), and anti–chromogranin A (A0430; DAKO, Glostrup, Denmark) antibodies in set 2. The TMA was examined by AQUA after immunofluorescent staining [32] (link) and by a pathologist (B. K.) after DAB staining (blinded to tissue labels). For automated analysis, neuroendocrine tumor cells or normal mucosal epithelia were identified using a fluorescently tagged mouse anti-cytokeratin antibody cocktail (AE1/AE3; DAKO), nuclei were visualized by 4′,6-diamidino-2-phenylindole, and targets were visualized with a fluorescent chromogen (Cy-5-tyramide; NEN Life Science Products, Boston, MA). AQUA expression values were quantified as low (below median) or high expression (above median).
Anti chromogranin a
Manufactured by Agilent Technologies
Sourced in Denmark
Anti-chromogranin A is a laboratory instrument used to detect and measure the levels of chromogranin A, a protein found in neuroendocrine cells. The core function of this product is to provide a quantitative analysis of chromogranin A levels, which can be useful in the diagnosis and monitoring of certain neuroendocrine conditions.
Automatically generated - may contain errors
3 protocols using anti chromogranin a
1
Quantitative Immunofluorescence Analysis of Neuroendocrine Tumor Markers
2
Lineage Tracing of CgA-Expressing Cells
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CgA-CreERT2 mice were crossed with Rosa-CAG-LSL-ZsGreen1 Cre-reporter mice. The offspring were intraperitoneally injected with tamoxifen (1 mg/body) dissolved in corn oil once daily for 3 days. The duodenum, pancreas, and brain were resected and fixed with 10% formalin. Paraffin-embedded tissue sections were immunostained with anti-chromogranin A, anti-insulin, and anti-NSE (DAKO, Glostrup, Denmark) as described [17 (link)]. The sections were visualized with fluorescently (Alexa 555) labeled secondary antibodies (Life Technologies) on a BZ9000 microscope (KEYENCE, Tokyo, Japan).
3
Immunohistochemical Analysis of Neuroendocrine Markers
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The following antibodies were used in this study: anti-PEG10 (GeneTex: 4C10A7, 1:400); anti-chromogranin A (Dako: N1535, 1:10), and anti-synaptophysin (Nichirei Bioscience: 413831, 1:2).
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