Sodium chromate, Triton X-100, demecolcine and potassium chloride (KCl) were purchased from Sigma Chemical (St. Louis, MO). Giemsa stain was purchased from Ricca Chemical Company. (Arlington, TX). Crystal violet, sodium dodecyl sulfate (SDS), and acetic acid were purchased from J.T. Baker (Phillipsburg, NJ). Nitric acid and methanol were purchased from BDH Chemicals (Radnor, PA). Gurr’s buffer, Keratinocyte-SFM, and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen Corporation (Grand Island, NY). Tissue culture dishes, flasks and plasticware were purchased from BD (Franklin Lakes, NJ). Urothelial cell medium, trypsin/EDTA and trypsin neutralizing solution were purchased from ScienCell Research Laboratories (Carlsbad, CA). Penicillin/streptomycin was purchased from Mediatech (Manassas, VA). Paraformaldehyde 4% was purchased from Alfa Aesar (Ward Hill, MA). Phospho-histone H2A.X (Ser139) antibody was purchased from Cell Signaling (Beverly, MA). AlexaFluor 488-conjugated goat anti-rabbit IgG secondary antibody was purchased from ThermoScientific (Rockford, IL).
Trypsin neutralizing solution
Manufactured by ScienCell
Trypsin neutralizing solution is a laboratory reagent used to inactivate the enzymatic activity of trypsin. Trypsin is commonly used to dissociate and detach cells from cell culture surfaces. The trypsin neutralizing solution contains components that inhibit the proteolytic action of trypsin, allowing for the effective termination of the cell dissociation process.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by ScienCell (2 mentions)
Methanol, by Avantor (1 mentions)
Paraformaldehyde 4, by Thermo Fisher Scientific (1 mentions)
Keratinocyte sfm, by Thermo Fisher Scientific (1 mentions)
Phospho histone h2a x ser139 antibody, by Cell Signaling Technology (1 mentions)
Sodium chromate, by Merck Group (1 mentions)
Gurr s buffer, by Thermo Fisher Scientific (1 mentions)
Demecolcine, by Merck Group (1 mentions)
Nitric acid, by Avantor (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit igg secondary antibody, by Thermo Fisher Scientific (1 mentions)
Urothelial cell medium, by ScienCell (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Acetic acid, by Avantor (1 mentions)
Crystal violet, by Avantor (1 mentions)
Sodium dodecyl sulfate (sds), by Avantor (1 mentions)
Tissue culture dishes, by BD (1 mentions)
Epithelial cell growth supplement, by ScienCell (1 mentions)
Hibec, by ScienCell (1 mentions)
2 protocols using trypsin neutralizing solution
1
Immunofluorescent Analysis of DNA Damage
2
Primary HSCs and HiBECs Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary human HSCs and primary human intrahepatic biliary epithelial cells (HiBECs, cholangiocytes) were purchased from ScienCell Research Laboratories (5300 & 4100, respectively). HSCs were cultured in stellate cell medium (ScienCell, 5301) supplemented with 2% fetal bovine serum (FBS), stellate cell growth supplements, and 1% penicillin/streptomycin (P/S). HiBECs were cultured in epithelial cell medium (ScienCell, 4101) supplemented with 2% FBS, epithelial cell growth supplement (ScienCell), and 1% P/S. Cells were grown in flasks precoated with poly-l -lysine and subcultured with trypsin/EDTA and trypsin neutralizing solution (ScienCell, 0103 & 0113, respectively) according to supplier protocol. LX2 cells (Merck, catalog SCC064) were cultured in DMEM, with 10% FCS. In cell culture experiments, supernatants were assayed for CCL24 using ELISA (R&D Systems, catalog DY343).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!