HCT116 and SW480 cells were obtained from American Type Culture Collection (Manassas, USA). HCT116 cells were cultured in McCoy's 5A medium (Invitrogen, Carlsbad, CA) and incubated at 5% CO2, 37°C and 95% humidity; SW480 cells were cultured in Leibovit's L-15 medium (Invitrogen) and incubated at 5% CO2, 37°C and 95% humidity. Both of these media were supplemented with 10% fetal bovine serum (HyClone, Ogden, UT) and 100 U/ml antibiotics (penicillin–streptomycin, Invitrogen). Hypoxic conditions were achieved by culturing cells in a sealed hypoxia chamber (Billups-Rothenberg, Del Mar, CA) with mixed gas containing 1% O2.
Leibovit s l 15 medium
Manufactured by Thermo Fisher Scientific
Leibovit's L-15 medium is a cell culture medium developed by John H. Leibovit. It is a formulation designed to support the growth and maintenance of various cell types in vitro. The medium provides the necessary nutrients, vitamins, and other essential components to sustain cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Hct116, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Sw480, by American Type Culture Collection (1 mentions)
Sw480, by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Sealed hypoxia chamber, by Embrient Inc (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Bt474, by American Type Culture Collection (1 mentions)
Bt474, by Thermo Fisher Scientific (1 mentions)
Bovine insulin, by Merck Group (1 mentions)
Bovine insulin, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids solution, by Thermo Fisher Scientific (1 mentions)
Mda mb 361, by Thermo Fisher Scientific (1 mentions)
Mda mb 361, by American Type Culture Collection (1 mentions)
Oxaloacetic acid, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem high glucose, by Thermo Fisher Scientific (1 mentions)
Nctc 135 medium, by Merck Group (1 mentions)
Non essential amino acids solution, by Nacalai Tesque (1 mentions)
2 protocols using leibovit s l 15 medium
1
Cell Culture Protocols for Colorectal Cancer
2
Culture Conditions for Breast Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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BT-474, MDA-MB-361, and MCF-7 human breast cancer cell lines were obtained from
the American Type Culture Collection (Manassas, VA, USA). MCF-7-Ac1, a human
breast cancer cell line that stably expresses aromatase, was kindly provided
from Professor A. Brodie (University of Maryland, Baltimore, MD, USA). BT-474
cells were maintained in culture medium [Dulbecco’s modified Eagle medium (DMEM;
high glucose, Invitrogen, Grand Island, NY, USA), 10% NCTC-135 medium
(Sigma-Aldrich, St. Louis, MO, USA), 0.01 mg/ml bovine insulin (Sigma-Aldrich),
1.2 mmol/l oxaloacetic acid (Sigma-Aldrich), and 10% heat-inactivated fetal
bovine serum (FBS; SAFC Biosciences, Lenexa, KS, USA)]. MDA-MB-361 cells were
maintained in Leibovit’s L-15 medium (Invitrogen) supplemented with 20%
heat-inactivated FBS. MCF-7 cells were maintained in RPMI 1640 medium
(Invitrogen) supplemented with 10% heat-inactivated FBS (Invitrogen), 1%
nonessential amino acids solution (Nacalai Tesque, Kyoto, Japan), 1 mmol/l
sodium pyruvate, and 0.01 mg/ml bovine insulin. MCF-7-Ac1 cells were maintained
in RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% nonessential
amino acids solution, 1 mmol/l sodium pyruvate, 0.01 mg/ml bovine insulin, and
800 μg/ml Geneticin® (Invitrogen).
the American Type Culture Collection (Manassas, VA, USA). MCF-7-Ac1, a human
breast cancer cell line that stably expresses aromatase, was kindly provided
from Professor A. Brodie (University of Maryland, Baltimore, MD, USA). BT-474
cells were maintained in culture medium [Dulbecco’s modified Eagle medium (DMEM;
high glucose, Invitrogen, Grand Island, NY, USA), 10% NCTC-135 medium
(Sigma-Aldrich, St. Louis, MO, USA), 0.01 mg/ml bovine insulin (Sigma-Aldrich),
1.2 mmol/l oxaloacetic acid (Sigma-Aldrich), and 10% heat-inactivated fetal
bovine serum (FBS; SAFC Biosciences, Lenexa, KS, USA)]. MDA-MB-361 cells were
maintained in Leibovit’s L-15 medium (Invitrogen) supplemented with 20%
heat-inactivated FBS. MCF-7 cells were maintained in RPMI 1640 medium
(Invitrogen) supplemented with 10% heat-inactivated FBS (Invitrogen), 1%
nonessential amino acids solution (Nacalai Tesque, Kyoto, Japan), 1 mmol/l
sodium pyruvate, and 0.01 mg/ml bovine insulin. MCF-7-Ac1 cells were maintained
in RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% nonessential
amino acids solution, 1 mmol/l sodium pyruvate, 0.01 mg/ml bovine insulin, and
800 μg/ml Geneticin® (Invitrogen).
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