One rat spinal cord was extracted via transcardial perfusion with 4% Paraformaldehyde (PFA). After extraction, the spinal cord was immersed in a 4% PFA solution for 24 h, and then washed in a Phosphate-Buffered Saline (PBS) solution for 24 h. Two sections of cervical spinal cord were cut and placed separately in 5 mm NMR tubes filled with Fluorinert (Sigma Aldrich, Lisbon, PT). The samples were imaged on a 16.4 T Bruker Aeon Ascend scanner (Bruker, Karlsruhe, Germany) equipped with a 5 mm birdcage coil and gradients capable of producing up to 3 T/m in all directions.
Aeon ascend scanner
Manufactured by Bruker
Sourced in Germany
The Aeon Ascend scanner is a laboratory equipment product offered by Bruker. It is designed to perform high-resolution scanning of samples. The core function of the Aeon Ascend scanner is to capture detailed images and data from the scanned objects.
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Lab products found in correlation
3 protocols using aeon ascend scanner
1
Spinal Cord Extraction and NMR Imaging
2
Spinal Cord Extraction and NMR Imaging
3
High-field ex-vivo MRI diffusion imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The ex-vivo MRI images were acquired on a 16.4T Bruker Aeon Ascend scanner interfaced with an AVANCE IIIHD console and operating a Micro5 probe with a gradient system capable of producing up to 3000 mT/m in all directions. A 10 mm birdcage coil was used for signal reception and RF transmission and samples were scanned at 37°C. The diffusion weighted images were acquired in stimulated echo acquisition mode (STEAM) using standard line-by-line readout with imaging parameters detailed in Table 1a. Single diffusion encoding (SDE) sequences with 6 gradient directions and duration δ = 1 ms were acquired at four b-values up to 2000 s/mm 2 and multiple diffusion times Δ from 5 to 150ms, as detailed in Table 1a, leading to a total scan time of ~6h per node.
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