M205 light microscope

A TDM modeling technique, yielding the parameters for time and dose effects of the paraquat was performed as previously described.¹⁴ Briefly, 30 synchronized L4-larval nematodes per well were picked out and raised in 96-well plates with different paraquat-treatment doses. The final concentrations of the tested paraquat were diluted from the original stock solution (0.1 M) as follows: 250 mM, 150 mM, 75 mM, 25 mM, and M9 buffer (same as described above) only, which was considered to be 0 mM. The total volume was 300 μL. All assay trials were performed at 20 °C and 12:12 (light: dark). The number of nematode survivors were recorded per hour and observation was continued for 8 hours. Morphology of nematodes treated with different doses of paraquat-poisoning was observed after exposing in paraquat for 1 hour using a Leica M205 light microscope. The condition of C. elegans in death or living was judged as described in Keith et al.^{8 (link)} All experiments were repeated 3 times.