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Alexa fluor 488 conjugated anti mouse rat immunoglobulin ig g

Manufactured by Abcam
Sourced in Japan

Alexa Fluor 488-conjugated anti-mouse rat immunoglobulin (Ig)G is a fluorescently-labeled secondary antibody that binds to mouse IgG from rat sources. The Alexa Fluor 488 dye provides green fluorescence for detection and visualization applications.

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2 protocols using alexa fluor 488 conjugated anti mouse rat immunoglobulin ig g

1

Immunohistochemical and Immunofluorescence Analysis of Immune Markers

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The following antibodies (Abs) were used for immunohistochemical staining: mouse monoclonal Abs (LifeSpan BioScience, Seattle, WA, United States) against human CCL20 and human IL-36γ, rabbit polyclonal Abs (LifeSpan BioScience) against human CYP1A1 and human IL-23, and a goat polyclonal Ab (R&D Systems, Minneapolis, MN, United States) against human IL-17. The following antibodies were used for immunofluorescence (IF): mouse anti-human CD163 phycoerythrin-conjugated monoclonal antibody (R&D Systems), rabbit polyclonal anti-CCL22 antibody (R&D Systems), rabbit polyclonal anti-CXCL5 antibody (Lifespan Bioscience, Seattle, WA, United States), mouse anti-CXCL10 antibody (Lifespan Bioscience), Alexa Fluor 488-conjugated anti-mouse rat immunoglobulin (Ig)G (Abcam, Tokyo, Japan), and Alexa Fluor 488-conjugated anti-rabbit goat IgG (Abcam).
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2

Immunofluorescence and Immunohistochemistry Protocols

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The following antibodies were used for immunofluorescence (IF): mouse anti-human CD163 phycoerythrin-conjugated monoclonal antibody (R&D Systems), rabbit polyclonal anti-CCL22 antibody (Biorbyt, Cambridge, UK), rabbit polyclonal anti-CXCL5 antibody (Lifespan Bioscience, Seattle, WA), mouse anti-CXCL10 antibody (Lifespan Bioscience), Alexa Fluor 488-conjugated anti-mouse rat immunoglobulin (Ig)G (Abcam, Tokyo, Japan), and Alexa Fluor 488-conjugated anti-rabbit goat IgG (Abcam). We used the following antibodies for immunohistochemical staining: rabbit polyclonal antibodies for human CCL22 (R&D Systems) and human CXCL5 (LifeSpan Bioscience).
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